Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication
A microfluidics-based device that has the capability to trap cells has been designed and constructed and its capabilities have been demonstrated using dielectrophoresis-a promising tool for the selective manipulation and separation of cells, bacteria and other bioparticles. The device consists of a...
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| Format: | Article |
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American Scientific Publishers
2012
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| _version_ | 1796945220678451200 |
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| author | Wee, Wei Hong Razak, Mohd Azhar Abdul Kadri, Nahrizul Adib |
| author_facet | Wee, Wei Hong Razak, Mohd Azhar Abdul Kadri, Nahrizul Adib |
| author_sort | Wee, Wei Hong |
| collection | UM |
| description | A microfluidics-based device that has the capability to trap cells has been designed and constructed and its capabilities have been demonstrated using dielectrophoresis-a promising tool for the selective manipulation and separation of cells, bacteria and other bioparticles. The device consists of a dielectrophoretic chamber and an interdigitated electrode geometry that is fabricated from gold-coated glass slides. It was designed with the intention of providing a device that was compatible with standard laboratory equipment and had the capability to prevent leakage and discriminate cells. Experimental results reveal that the device is capable of trapping leukemic K562 cells at an average efficiency of 94.2. In addition to this, the design offers a number of advantages, which include the ability to exchange microscope slides and change separation chamber height. It is also reusable, easy to assembly and cleaning and provides a simple method of connecting electrodes and a power source. The device has the potential to become very beneficial in the preliminary stages of many diagnostic and immunosensing applications that require separation of different types of cells. © 2012 American Scientific Publishers All rights reserved. |
| first_indexed | 2024-03-06T05:13:25Z |
| format | Article |
| id | um.eprints-5039 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:13:25Z |
| publishDate | 2012 |
| publisher | American Scientific Publishers |
| record_format | dspace |
| spelling | um.eprints-50392021-01-20T08:21:40Z http://eprints.um.edu.my/5039/ Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication Wee, Wei Hong Razak, Mohd Azhar Abdul Kadri, Nahrizul Adib T Technology (General) A microfluidics-based device that has the capability to trap cells has been designed and constructed and its capabilities have been demonstrated using dielectrophoresis-a promising tool for the selective manipulation and separation of cells, bacteria and other bioparticles. The device consists of a dielectrophoretic chamber and an interdigitated electrode geometry that is fabricated from gold-coated glass slides. It was designed with the intention of providing a device that was compatible with standard laboratory equipment and had the capability to prevent leakage and discriminate cells. Experimental results reveal that the device is capable of trapping leukemic K562 cells at an average efficiency of 94.2. In addition to this, the design offers a number of advantages, which include the ability to exchange microscope slides and change separation chamber height. It is also reusable, easy to assembly and cleaning and provides a simple method of connecting electrodes and a power source. The device has the potential to become very beneficial in the preliminary stages of many diagnostic and immunosensing applications that require separation of different types of cells. © 2012 American Scientific Publishers All rights reserved. American Scientific Publishers 2012 Article PeerReviewed Wee, Wei Hong and Razak, Mohd Azhar Abdul and Kadri, Nahrizul Adib (2012) Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication. Advanced Science Letters, 15 (1). pp. 1-4. ISSN 1936-6612, |
| spellingShingle | T Technology (General) Wee, Wei Hong Razak, Mohd Azhar Abdul Kadri, Nahrizul Adib Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication |
| title | Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication |
| title_full | Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication |
| title_fullStr | Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication |
| title_full_unstemmed | Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication |
| title_short | Dielectrophoretic K562 cell entrapment device using benchtop microfluidics fabrication |
| title_sort | dielectrophoretic k562 cell entrapment device using benchtop microfluidics fabrication |
| topic | T Technology (General) |
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