Ammonium uptake and growth kinetics of pseudomonas putida PGA1
Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Molecular Biology and Biotechnology
2006
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/5373/1/Ammonium_uptake_and_growth_kinetics_of_Pseudomonas_putida_PGA1.pdf |
| _version_ | 1825719084703023104 |
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| author | Annuar, Mohamad Suffian Mohamad Tan, Irene Kit Ping Ibrahim, Shaliza Ramachandran, K.B. |
| author_facet | Annuar, Mohamad Suffian Mohamad Tan, Irene Kit Ping Ibrahim, Shaliza Ramachandran, K.B. |
| author_sort | Annuar, Mohamad Suffian Mohamad |
| collection | UM |
| description | Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the micro-organism’s specific uptake rate of ammonium and its growth would increase as the ammonium ion concentrations become higher. Optimal ammonium uptake and growth rates were observed at 0.1 gL-1 ammonium ion in aqueous medium. It also indicated that the ammonium transport mechanism is probably inducible by ammonium concentration. The limit of ammonium ion level increase before cells stop growing completely is given by the substrate inhibition constant, Ki which equals to 1.2 gL-1 ammonium ion concentration. |
| first_indexed | 2024-03-06T05:14:15Z |
| format | Article |
| id | um.eprints-5373 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:14:15Z |
| publishDate | 2006 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | um.eprints-53732020-05-29T02:45:31Z http://eprints.um.edu.my/5373/ Ammonium uptake and growth kinetics of pseudomonas putida PGA1 Annuar, Mohamad Suffian Mohamad Tan, Irene Kit Ping Ibrahim, Shaliza Ramachandran, K.B. Q Science (General) QR Microbiology Ammonium uptake kinetics of low specific growth rate cultures of Pseudomonas putida PGA1, a gram negative fluorescent pseudomonad, which is an important producer of poly(3-hydroxyalkanoates) (PHA) molecules, were studied. The ammonium uptake follows a first-order kinetics model, indicating that the micro-organism’s specific uptake rate of ammonium and its growth would increase as the ammonium ion concentrations become higher. Optimal ammonium uptake and growth rates were observed at 0.1 gL-1 ammonium ion in aqueous medium. It also indicated that the ammonium transport mechanism is probably inducible by ammonium concentration. The limit of ammonium ion level increase before cells stop growing completely is given by the substrate inhibition constant, Ki which equals to 1.2 gL-1 ammonium ion concentration. Malaysian Society for Molecular Biology and Biotechnology 2006 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5373/1/Ammonium_uptake_and_growth_kinetics_of_Pseudomonas_putida_PGA1.pdf Annuar, Mohamad Suffian Mohamad and Tan, Irene Kit Ping and Ibrahim, Shaliza and Ramachandran, K.B. (2006) Ammonium uptake and growth kinetics of pseudomonas putida PGA1. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 1-10. ISSN 0128-7451, http://www.msmbb.my/index.php/memberships/subscription/2-uncategorised/302-abstract-ammonium-uptake-and-growth-kinetics-of-pseudomonas-putida-pga1 |
| spellingShingle | Q Science (General) QR Microbiology Annuar, Mohamad Suffian Mohamad Tan, Irene Kit Ping Ibrahim, Shaliza Ramachandran, K.B. Ammonium uptake and growth kinetics of pseudomonas putida PGA1 |
| title | Ammonium uptake and growth kinetics of pseudomonas putida PGA1 |
| title_full | Ammonium uptake and growth kinetics of pseudomonas putida PGA1 |
| title_fullStr | Ammonium uptake and growth kinetics of pseudomonas putida PGA1 |
| title_full_unstemmed | Ammonium uptake and growth kinetics of pseudomonas putida PGA1 |
| title_short | Ammonium uptake and growth kinetics of pseudomonas putida PGA1 |
| title_sort | ammonium uptake and growth kinetics of pseudomonas putida pga1 |
| topic | Q Science (General) QR Microbiology |
| url | http://eprints.um.edu.my/5373/1/Ammonium_uptake_and_growth_kinetics_of_Pseudomonas_putida_PGA1.pdf |
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