Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia
Background and aims: Salmonella infections remain a major public health problem in developing countries. The occurrence of infections caused by antimicrobial-resistant Salmonella has been on the rise complicating the available therapeutic options. The study aimed to determine the antibiograms and ge...
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| Format: | Article |
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Elsevier
2011
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| _version_ | 1825719095203463168 |
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| author | Thong, Kwai Lin Lai, Wai Ling Dhanoa, Amreeta |
| author_facet | Thong, Kwai Lin Lai, Wai Ling Dhanoa, Amreeta |
| author_sort | Thong, Kwai Lin |
| collection | UM |
| description | Background and aims: Salmonella infections remain a major public health problem in developing countries. The occurrence of infections caused by antimicrobial-resistant Salmonella has been on the rise complicating the available therapeutic options. The study aimed to determine the antibiograms and genotypes of prevalent Salmonella serotypes. Methods: A retrospective study involving 80 stool and extra-intestinal Salmonella strains collected over a 18-month period (January 2005-June 2006) from a tertiary hospital in Penang, Malaysia was conducted. Isolates were examined for resistance to 14 antimicrobial drugs and the clonality of the strains was determined by PFGE. Results: Twenty-one serotypes were identified, the most common being S. enteritidis (42.5%) followed by S. corvallis (11.25%) and S. braenderup (11.25%). S. enteritidis was significantly more common amongst the extra-intestinal isolates compared to stool isolates (74.2% versus 22.4%, p< 0.0001). Overall, the highest resistance was observed for tetracycline (66.3%), sulphonamides (56.3%), streptomycin (32.5%), trimethoprim (28.8%) and nalidixic acid (27.5%). Amongst the 31 invasive extra-intestinal isolates, resistance towards therapeutically relevant antibiotics was as follows: co-trimoxazole (38.7%), ampicillin (29%) and ceftriaxone (3.2%). Although there was no detectable resistance towards chloramphenicol and ciprofloxacin, 29% strains showed nalidixic acid resistance. About 41% of the 80 isolates were multidrug-resistant. PFGE subtyped the 78 Salmonella isolates to 33 distinct XbaI-pulsotypes. Isolates within the serotypes S. enteritidis, S. corvallis, S. branderup and S. fasta were more homogeneous while S. typhi and S. weltervden were genetically more diverse. Conclusions: The high percentage of multidrug-resistant Salmonella strains is worrying and is of public health concern. PFGE was a useful and discriminative method for assessing the genetic diversity of Salmonellae. © 2011 King Saud Bin Abdulaziz University for Health Sciences. |
| first_indexed | 2024-03-06T05:14:25Z |
| format | Article |
| id | um.eprints-5437 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:14:25Z |
| publishDate | 2011 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-54372019-11-14T03:40:56Z http://eprints.um.edu.my/5437/ Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia Thong, Kwai Lin Lai, Wai Ling Dhanoa, Amreeta Q Science (General) QH Natural history QR Microbiology Background and aims: Salmonella infections remain a major public health problem in developing countries. The occurrence of infections caused by antimicrobial-resistant Salmonella has been on the rise complicating the available therapeutic options. The study aimed to determine the antibiograms and genotypes of prevalent Salmonella serotypes. Methods: A retrospective study involving 80 stool and extra-intestinal Salmonella strains collected over a 18-month period (January 2005-June 2006) from a tertiary hospital in Penang, Malaysia was conducted. Isolates were examined for resistance to 14 antimicrobial drugs and the clonality of the strains was determined by PFGE. Results: Twenty-one serotypes were identified, the most common being S. enteritidis (42.5%) followed by S. corvallis (11.25%) and S. braenderup (11.25%). S. enteritidis was significantly more common amongst the extra-intestinal isolates compared to stool isolates (74.2% versus 22.4%, p< 0.0001). Overall, the highest resistance was observed for tetracycline (66.3%), sulphonamides (56.3%), streptomycin (32.5%), trimethoprim (28.8%) and nalidixic acid (27.5%). Amongst the 31 invasive extra-intestinal isolates, resistance towards therapeutically relevant antibiotics was as follows: co-trimoxazole (38.7%), ampicillin (29%) and ceftriaxone (3.2%). Although there was no detectable resistance towards chloramphenicol and ciprofloxacin, 29% strains showed nalidixic acid resistance. About 41% of the 80 isolates were multidrug-resistant. PFGE subtyped the 78 Salmonella isolates to 33 distinct XbaI-pulsotypes. Isolates within the serotypes S. enteritidis, S. corvallis, S. branderup and S. fasta were more homogeneous while S. typhi and S. weltervden were genetically more diverse. Conclusions: The high percentage of multidrug-resistant Salmonella strains is worrying and is of public health concern. PFGE was a useful and discriminative method for assessing the genetic diversity of Salmonellae. © 2011 King Saud Bin Abdulaziz University for Health Sciences. Elsevier 2011 Article PeerReviewed Thong, Kwai Lin and Lai, Wai Ling and Dhanoa, Amreeta (2011) Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia. Journal of Infection and Public Health, 4 (2). pp. 65-72. ISSN 1876-0341, DOI https://doi.org/10.1016/j.jiph.2011.03.003 <https://doi.org/10.1016/j.jiph.2011.03.003>. https://doi.org/10.1016/j.jiph.2011.03.003 doi:10.1016/j.jiph.2011.03.003 |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Thong, Kwai Lin Lai, Wai Ling Dhanoa, Amreeta Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia |
| title | Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia |
| title_full | Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia |
| title_fullStr | Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia |
| title_full_unstemmed | Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia |
| title_short | Antimicrobial susceptibility and pulsed - Field Gel Electrophoretic analysis of Salmonella in a tertiary hospital in northern Malaysia |
| title_sort | antimicrobial susceptibility and pulsed field gel electrophoretic analysis of salmonella in a tertiary hospital in northern malaysia |
| topic | Q Science (General) QH Natural history QR Microbiology |
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