Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia
Pulse-Field Gel Electrophoresis (PFGE) and BOX-PCR patterns were used to determine the phylogenetic relationships among 32 strains of Ralstonia solanacearum collected from banana farms in West Malaysia. Eighty two percent (25 stains) were biovar 3 and the remainders were biovar 4. Cluster analysis b...
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| Format: | Article |
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Asian Network for Scientific Information
2008
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| author | Khakvar, R. Sijam, K. Wong, M.Y. Radu, S. Jones, J. Thong, Kwai Lin |
| author_facet | Khakvar, R. Sijam, K. Wong, M.Y. Radu, S. Jones, J. Thong, Kwai Lin |
| author_sort | Khakvar, R. |
| collection | UM |
| description | Pulse-Field Gel Electrophoresis (PFGE) and BOX-PCR patterns were used to determine the phylogenetic relationships among 32 strains of Ralstonia solanacearum collected from banana farms in West Malaysia. Eighty two percent (25 stains) were biovar 3 and the remainders were biovar 4. Cluster analysis based on PFGE and BOX-PCR fingerprinting showed that there was significant genetic variation among all strains within this species in West Malaysia. Patterns of DNA fingerprinting in both methods were significantly correlated with sampling sites and revealed the regional similarity among the strains. The PFGE patterns varied from 78 to 99 while BOX-PCR patterns showed a high degree of variation among R. solanacearum strains. Genomic fingerprinting by PFGE also revealed one that was significantly correlated to biovar type while BOX-PCR pattern analysis was unable to differentiate various biovars. This study clearly showed that R. solanacearum strains were phylogenetically similar within a region but diverse between regions despite biovar designation. |
| first_indexed | 2024-03-06T05:14:38Z |
| format | Article |
| id | um.eprints-5535 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:14:38Z |
| publishDate | 2008 |
| publisher | Asian Network for Scientific Information |
| record_format | dspace |
| spelling | um.eprints-55352018-10-15T02:49:49Z http://eprints.um.edu.my/5535/ Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia Khakvar, R. Sijam, K. Wong, M.Y. Radu, S. Jones, J. Thong, Kwai Lin Q Science (General) QH Natural history QR Microbiology Pulse-Field Gel Electrophoresis (PFGE) and BOX-PCR patterns were used to determine the phylogenetic relationships among 32 strains of Ralstonia solanacearum collected from banana farms in West Malaysia. Eighty two percent (25 stains) were biovar 3 and the remainders were biovar 4. Cluster analysis based on PFGE and BOX-PCR fingerprinting showed that there was significant genetic variation among all strains within this species in West Malaysia. Patterns of DNA fingerprinting in both methods were significantly correlated with sampling sites and revealed the regional similarity among the strains. The PFGE patterns varied from 78 to 99 while BOX-PCR patterns showed a high degree of variation among R. solanacearum strains. Genomic fingerprinting by PFGE also revealed one that was significantly correlated to biovar type while BOX-PCR pattern analysis was unable to differentiate various biovars. This study clearly showed that R. solanacearum strains were phylogenetically similar within a region but diverse between regions despite biovar designation. Asian Network for Scientific Information 2008 Article PeerReviewed Khakvar, R. and Sijam, K. and Wong, M.Y. and Radu, S. and Jones, J. and Thong, Kwai Lin (2008) Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia. Plant Pathology Journal, 7 (2). pp. 162-167. ISSN 1812-5387, DOI https://doi.org/10.3923/ppj.2008.162.167 <https://doi.org/10.3923/ppj.2008.162.167>. http://dx.doi.org/10.3923/ppj.2008.162.167 doi:10.3923/ppj.2008.162.167 |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Khakvar, R. Sijam, K. Wong, M.Y. Radu, S. Jones, J. Thong, Kwai Lin Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia |
| title | Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia |
| title_full | Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia |
| title_fullStr | Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia |
| title_full_unstemmed | Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia |
| title_short | Genomic Diversity of Ralstonia Solanacearum Strains Isolated from Banana Farms in West Malaysia |
| title_sort | genomic diversity of ralstonia solanacearum strains isolated from banana farms in west malaysia |
| topic | Q Science (General) QH Natural history QR Microbiology |
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