Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping
Amplified fragment length polymorphism (AFLP) is a recently developed, PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In the present study, AFLP was evaluated for its...
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| Format: | Article |
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2000
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| author | Nair, S. Schreiber, E. Thong, Kwai Lin Pang, T. Altwegg, M. |
| author_facet | Nair, S. Schreiber, E. Thong, Kwai Lin Pang, T. Altwegg, M. |
| author_sort | Nair, S. |
| collection | UM |
| description | Amplified fragment length polymorphism (AFLP) is a recently developed, PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In the present study, AFLP was evaluated for its usefulness in the molecular typing of Salmonella typhi in comparison to ribotyping and pulsed-field gel electrophoresis (PFGE). Six S. typhi isolates from diverse geographic areas (Malaysia, Indonesia, India, Chile, Papua New Guinea and Switzerland) gave unique, heterogeneous profiles when typed by AFLP, a result which was consistent with ribotyping and PFGE analysis. In a further study of selected S. typhi isolates from Papua New Guinea which caused fatal and non-fatal disease previously shown to be clonally related by PFGE, AFLP discriminated between these isolates but did not indicate a linkage between genotype with virulence. We conclude that AFLP (discriminatory index = 0.88) has a higher discriminatory power for strain differentiation among S. typhi than ribotyping (DI = 0.63) and PFGE (DI = 0.74). (C) 2000 Elsevier Science B.V. All rights reserved. |
| first_indexed | 2024-03-06T05:14:38Z |
| format | Article |
| id | um.eprints-5538 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:14:38Z |
| publishDate | 2000 |
| record_format | dspace |
| spelling | um.eprints-55382018-10-12T06:29:47Z http://eprints.um.edu.my/5538/ Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping Nair, S. Schreiber, E. Thong, Kwai Lin Pang, T. Altwegg, M. Q Science (General) QR Microbiology Amplified fragment length polymorphism (AFLP) is a recently developed, PCR-based high resolution fingerprinting method that is able to generate complex banding patterns which can be used to delineate intraspecific genetic relationships among bacteria. In the present study, AFLP was evaluated for its usefulness in the molecular typing of Salmonella typhi in comparison to ribotyping and pulsed-field gel electrophoresis (PFGE). Six S. typhi isolates from diverse geographic areas (Malaysia, Indonesia, India, Chile, Papua New Guinea and Switzerland) gave unique, heterogeneous profiles when typed by AFLP, a result which was consistent with ribotyping and PFGE analysis. In a further study of selected S. typhi isolates from Papua New Guinea which caused fatal and non-fatal disease previously shown to be clonally related by PFGE, AFLP discriminated between these isolates but did not indicate a linkage between genotype with virulence. We conclude that AFLP (discriminatory index = 0.88) has a higher discriminatory power for strain differentiation among S. typhi than ribotyping (DI = 0.63) and PFGE (DI = 0.74). (C) 2000 Elsevier Science B.V. All rights reserved. 2000 Article PeerReviewed Nair, S. and Schreiber, E. and Thong, Kwai Lin and Pang, T. and Altwegg, M. (2000) Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping. Journal of Microbiological Methods, 41 (1). pp. 35-43. ISSN 0167-7012, DOI https://doi.org/10.1016/s0167-7012(00)00148-2 <https://doi.org/10.1016/s0167-7012(00)00148-2>. 10.1016/s0167-7012(00)00148-2 |
| spellingShingle | Q Science (General) QR Microbiology Nair, S. Schreiber, E. Thong, Kwai Lin Pang, T. Altwegg, M. Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping |
| title | Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping |
| title_full | Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping |
| title_fullStr | Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping |
| title_full_unstemmed | Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping |
| title_short | Genotypic Characterization of Salmonella typhi by Amplified Fragment Length Polymorphism Fingerprinting Provides Increased Discrimination as Compared to Pulsed-Field Gel Electrophoresis and Ribotyping |
| title_sort | genotypic characterization of salmonella typhi by amplified fragment length polymorphism fingerprinting provides increased discrimination as compared to pulsed field gel electrophoresis and ribotyping |
| topic | Q Science (General) QR Microbiology |
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