Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia
Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it's considered as a plant pathogen, R. solanacea...
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| Format: | Article |
| Language: | English |
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Science Publications
2008
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| Online Access: | http://eprints.um.edu.my/5542/1/Improving_a_PCR%E2%80%93Based_Method_for_Identification_of_Ralstonia_Solanacearum_in_Natural_Sources_of_West_Malaysia.pdf |
| _version_ | 1796945304188092416 |
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| author | Khakvar, Reza Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Thong, Kwai Lin |
| author_facet | Khakvar, Reza Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Thong, Kwai Lin |
| author_sort | Khakvar, Reza |
| collection | UM |
| description | Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it's considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various natural habitats such as surface waters and different types of soils. Epidemiological and ecological studies on pathogen distribution in the environment are seriously hindered by the lack of efficient detection method especially when the concentration of the pathogen is either very low or is present in a latent, dormant or non-culturable state. With due attention to importance of R. solanacearum in Malaysia and several irreparable losses that every year caused by this bacterium, this is prompted current study to develop a sensitive PCR-Based method to improve the detection of R. solanacearum in natural sources. We selected the previously reported primers (OLI1;OLI2; Y2; JE2) for their sensitivity and specificity detection of the bacterium in water and soil by a modification of PCR assay. |
| first_indexed | 2024-03-06T05:14:39Z |
| format | Article |
| id | um.eprints-5542 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:14:39Z |
| publishDate | 2008 |
| publisher | Science Publications |
| record_format | dspace |
| spelling | um.eprints-55422018-10-15T02:53:09Z http://eprints.um.edu.my/5542/ Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia Khakvar, Reza Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Thong, Kwai Lin Q Science (General) QH Natural history QR Microbiology Ralstonia solanacearum, a soil-borne plant pathogen, causes lethal wilting disease of more than 200 plants worldwide. This very wide host range covers both monocots and dicots, extending from annual plants to trees and shrubs. Although generally it's considered as a plant pathogen, R. solanacearum behaves primarily as a saprophytic bacterium able to survive for long periods of time in various natural habitats such as surface waters and different types of soils. Epidemiological and ecological studies on pathogen distribution in the environment are seriously hindered by the lack of efficient detection method especially when the concentration of the pathogen is either very low or is present in a latent, dormant or non-culturable state. With due attention to importance of R. solanacearum in Malaysia and several irreparable losses that every year caused by this bacterium, this is prompted current study to develop a sensitive PCR-Based method to improve the detection of R. solanacearum in natural sources. We selected the previously reported primers (OLI1;OLI2; Y2; JE2) for their sensitivity and specificity detection of the bacterium in water and soil by a modification of PCR assay. Science Publications 2008 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5542/1/Improving_a_PCR%E2%80%93Based_Method_for_Identification_of_Ralstonia_Solanacearum_in_Natural_Sources_of_West_Malaysia.pdf Khakvar, Reza and Sijam, Kamaruzaman and Wong, Mui Yun and Radu, Son and Thong, Kwai Lin (2008) Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia. American Journal of Agricultural and Biological Sciences, 3 (2). pp. 490-493. ISSN 1557-4997, DOI https://doi.org/10.3844/ajabssp.2008.490.493 <https://doi.org/10.3844/ajabssp.2008.490.493>. https://doi.org/10.3844/ajabssp.2008.490.493 doi:10.3844/ajabssp.2008.490.493 |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Khakvar, Reza Sijam, Kamaruzaman Wong, Mui Yun Radu, Son Thong, Kwai Lin Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia |
| title | Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia |
| title_full | Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia |
| title_fullStr | Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia |
| title_full_unstemmed | Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia |
| title_short | Improving a PCR-Based Method for Identification of Ralstonia Solanacearum in Natural Sources of West Malaysia |
| title_sort | improving a pcr based method for identification of ralstonia solanacearum in natural sources of west malaysia |
| topic | Q Science (General) QH Natural history QR Microbiology |
| url | http://eprints.um.edu.my/5542/1/Improving_a_PCR%E2%80%93Based_Method_for_Identification_of_Ralstonia_Solanacearum_in_Natural_Sources_of_West_Malaysia.pdf |
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