Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis
Pulsed-field gel electrophoresis (PFGE) was applied to analyse sporadic and outbreak cases of cholera caused by V. cholerae O1 biotype El Tor from Vietnam (n=16) and V. cholerae O139 from Malaysia (n=12) and from five vaccine strains. NotI-Digestion of chromosomal DNA from these isolates, followed b...
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Malaysian Society for Molecular Biology and Biotechnology
2002
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| author | Thong, Kwai Lin Goh, Yee Ling Phung, Dac Cam |
| author_facet | Thong, Kwai Lin Goh, Yee Ling Phung, Dac Cam |
| author_sort | Thong, Kwai Lin |
| collection | UM |
| description | Pulsed-field gel electrophoresis (PFGE) was applied to analyse sporadic and outbreak cases of cholera caused by V. cholerae O1 biotype El Tor from Vietnam (n=16) and V. cholerae O139 from Malaysia (n=12) and from five vaccine strains. NotI-Digestion of chromosomal DNA from these isolates, followed by PFGE produced eleven different profiles among the 16 V. cholerae O1 isolates (Dice coefficient: 0.67-0.97). The V. cholerae O1 Ogawa and Inaba strains were clearly differentiated into distinct clusters. NotI-PFGE analysis among V. cholerae O139 isolates showed a predominant PFGE profile (N12) indicating that the outbreak was probably derived a single clone. Among the five vaccine strains, the PFGE profiles of V. cholerae Inaba Cairo 48 and classical Inaba Cairo 48 were similar to some of the field isolates of 01 Inaba strains in Vietnam. The PFGE analysis showed that the V. cholerae O139 isolates from Malaysia were indistinguishable as these strains were probably derived from a cholera outbreak and the V. cholerae O1 isolates from Vietnam exhibited limited genetic diversity. |
| first_indexed | 2024-03-06T05:14:44Z |
| format | Article |
| id | um.eprints-5586 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:14:44Z |
| publishDate | 2002 |
| publisher | Malaysian Society for Molecular Biology and Biotechnology |
| record_format | dspace |
| spelling | um.eprints-55862019-02-11T07:47:13Z http://eprints.um.edu.my/5586/ Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis Thong, Kwai Lin Goh, Yee Ling Phung, Dac Cam Q Science (General) QR Microbiology Pulsed-field gel electrophoresis (PFGE) was applied to analyse sporadic and outbreak cases of cholera caused by V. cholerae O1 biotype El Tor from Vietnam (n=16) and V. cholerae O139 from Malaysia (n=12) and from five vaccine strains. NotI-Digestion of chromosomal DNA from these isolates, followed by PFGE produced eleven different profiles among the 16 V. cholerae O1 isolates (Dice coefficient: 0.67-0.97). The V. cholerae O1 Ogawa and Inaba strains were clearly differentiated into distinct clusters. NotI-PFGE analysis among V. cholerae O139 isolates showed a predominant PFGE profile (N12) indicating that the outbreak was probably derived a single clone. Among the five vaccine strains, the PFGE profiles of V. cholerae Inaba Cairo 48 and classical Inaba Cairo 48 were similar to some of the field isolates of 01 Inaba strains in Vietnam. The PFGE analysis showed that the V. cholerae O139 isolates from Malaysia were indistinguishable as these strains were probably derived from a cholera outbreak and the V. cholerae O1 isolates from Vietnam exhibited limited genetic diversity. Malaysian Society for Molecular Biology and Biotechnology 2002 Article PeerReviewed Thong, Kwai Lin and Goh, Yee Ling and Phung, Dac Cam (2002) Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis. Asia Pacific Journal of Molecular Biology and Biotechnology, 10 (2). pp. 103-108. ISSN 0128-7451, http://www.msmbb.my/index.php/publication |
| spellingShingle | Q Science (General) QR Microbiology Thong, Kwai Lin Goh, Yee Ling Phung, Dac Cam Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis |
| title | Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis |
| title_full | Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis |
| title_fullStr | Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis |
| title_full_unstemmed | Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis |
| title_short | Molecular subtyping of clinical isolates vibrio cholerae from Malaysia and Vietnam by pulsed-field gel electrophoresis |
| title_sort | molecular subtyping of clinical isolates vibrio cholerae from malaysia and vietnam by pulsed field gel electrophoresis |
| topic | Q Science (General) QR Microbiology |
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