Role of Molecular Typing in an Outbreak of Salmonella paratyphi A
During the post monsoon season of 1996 an outbreak of human Salmonellosis caused by Salmonella serovar-paratyphi A occurred in New Delhi and had continued for over 2 months. A total of 36 clinically diagnosed enteric-fever cases were reported during this outbreak. The isolates were compared followin...
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Digestive Diseases Foundation
2000
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| author | Chandel, D.S. Nisar, N. Thong, Kwai Lin Pang, T. Chaudhry, R. |
| author_facet | Chandel, D.S. Nisar, N. Thong, Kwai Lin Pang, T. Chaudhry, R. |
| author_sort | Chandel, D.S. |
| collection | UM |
| description | During the post monsoon season of 1996 an outbreak of human Salmonellosis caused by Salmonella serovar-paratyphi A occurred in New Delhi and had continued for over 2 months. A total of 36 clinically diagnosed enteric-fever cases were reported during this outbreak. The isolates were compared following their characterisation by biotyping, antibiogram-analysis, plasmid-profiling and IS200 probing, to study the relatedness in order to delineate a common source. The study included representative strains from both outbreak (15) and sporadic (7) cases for comparative analysis. Biotyping, antibiogram, whole cell protein-analysis and plasmid-profiling could not discriminate sporadic cases from outbreak strains, suggesting that a single clone/type (PT-1) may be prevalent in our region. In contrast, molecular-typing using IS200-probing revealed 2 clonally related strains circulating during the outbreak, as compared to the unrelated sporadic strains which exhibited considerable genetic diversity. Molecular analysis by IS200-probing, helped to assign an index case which provided a history of later outbreaks, since paratyphi A was repeatedly cultured in later outbreaks also. The study also suggests that genetic rearrangements can occur during the emergence of outbreaks. It reaffirmed the usefulness of IS200-probing in epidemiological investigations of Salmonella enterica serovars. |
| first_indexed | 2024-03-06T05:14:50Z |
| format | Article |
| id | um.eprints-5623 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:14:50Z |
| publishDate | 2000 |
| publisher | Digestive Diseases Foundation |
| record_format | dspace |
| spelling | um.eprints-56232018-10-12T06:30:36Z http://eprints.um.edu.my/5623/ Role of Molecular Typing in an Outbreak of Salmonella paratyphi A Chandel, D.S. Nisar, N. Thong, Kwai Lin Pang, T. Chaudhry, R. Q Science (General) QR Microbiology During the post monsoon season of 1996 an outbreak of human Salmonellosis caused by Salmonella serovar-paratyphi A occurred in New Delhi and had continued for over 2 months. A total of 36 clinically diagnosed enteric-fever cases were reported during this outbreak. The isolates were compared following their characterisation by biotyping, antibiogram-analysis, plasmid-profiling and IS200 probing, to study the relatedness in order to delineate a common source. The study included representative strains from both outbreak (15) and sporadic (7) cases for comparative analysis. Biotyping, antibiogram, whole cell protein-analysis and plasmid-profiling could not discriminate sporadic cases from outbreak strains, suggesting that a single clone/type (PT-1) may be prevalent in our region. In contrast, molecular-typing using IS200-probing revealed 2 clonally related strains circulating during the outbreak, as compared to the unrelated sporadic strains which exhibited considerable genetic diversity. Molecular analysis by IS200-probing, helped to assign an index case which provided a history of later outbreaks, since paratyphi A was repeatedly cultured in later outbreaks also. The study also suggests that genetic rearrangements can occur during the emergence of outbreaks. It reaffirmed the usefulness of IS200-probing in epidemiological investigations of Salmonella enterica serovars. Digestive Diseases Foundation 2000 Article PeerReviewed Chandel, D.S. and Nisar, N. and Thong, Kwai Lin and Pang, T. and Chaudhry, R. (2000) Role of Molecular Typing in an Outbreak of Salmonella paratyphi A. Tropical Gastroenterology, 21 (3). pp. 121-123. ISSN 0250-636X, |
| spellingShingle | Q Science (General) QR Microbiology Chandel, D.S. Nisar, N. Thong, Kwai Lin Pang, T. Chaudhry, R. Role of Molecular Typing in an Outbreak of Salmonella paratyphi A |
| title | Role of Molecular Typing in an Outbreak of Salmonella paratyphi A |
| title_full | Role of Molecular Typing in an Outbreak of Salmonella paratyphi A |
| title_fullStr | Role of Molecular Typing in an Outbreak of Salmonella paratyphi A |
| title_full_unstemmed | Role of Molecular Typing in an Outbreak of Salmonella paratyphi A |
| title_short | Role of Molecular Typing in an Outbreak of Salmonella paratyphi A |
| title_sort | role of molecular typing in an outbreak of salmonella paratyphi a |
| topic | Q Science (General) QR Microbiology |
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