Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia
A representative sample of 20 isolates of Salmonella weltevreden strains from stool cultures of patients admitted at the University Hospital, Kuala Lumpur, Malaysia were analyzed. All the strains were susceptible to ampicillin, ceftriaxone, ciprofloxacin, chloramphenicol, tetracycline, trimethoprim,...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
SEAMEO Regional Tropical Medicine and Public Health Network
2000
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/5650/1/Application_of_ribosomal_RNA_gene_restriction_patterns_analysis_and_pulsed-field_gel_electrophoresis_in_distinguishing_Salmonella_weltevreden_isolates_in_Malaysia..pdf |
| _version_ | 1825719125994897408 |
|---|---|
| author | Goh, Yee Ling Puthucheary, Savithri Devi Thong, Kwai Lin |
| author_facet | Goh, Yee Ling Puthucheary, Savithri Devi Thong, Kwai Lin |
| author_sort | Goh, Yee Ling |
| collection | UM |
| description | A representative sample of 20 isolates of Salmonella weltevreden strains from stool cultures of patients admitted at the University Hospital, Kuala Lumpur, Malaysia were analyzed. All the strains were susceptible to ampicillin, ceftriaxone, ciprofloxacin, chloramphenicol, tetracycline, trimethoprim, gentamicin and co-trimoxazole. Ribosomal RNA gene restriction pattern analysis of PstI-digested DNA gave three ribotypes while pulsed-field gel electrophoresis (PFGE) analysis of XbaI-digested DNA gave ten distinct profiles. PFGE was more discriminative than ribotyping in distinguishing the strains. The majority of the strains analyzed were very closely related with similarity coefficient values ranging from 0.8 to 1.0. Both PFGE and ribotyping could distinguish one of the strains which was obtained from a patient following a bone marrow transplant for β-thalassemia major, indicating that this particular strain was unrelated to the rest of the strains from patients with acute gastroenteritis. |
| first_indexed | 2024-03-06T05:14:53Z |
| format | Article |
| id | um.eprints-5650 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:14:53Z |
| publishDate | 2000 |
| publisher | SEAMEO Regional Tropical Medicine and Public Health Network |
| record_format | dspace |
| spelling | um.eprints-56502021-04-12T04:14:46Z http://eprints.um.edu.my/5650/ Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia Goh, Yee Ling Puthucheary, Savithri Devi Thong, Kwai Lin Q Science (General) QR Microbiology A representative sample of 20 isolates of Salmonella weltevreden strains from stool cultures of patients admitted at the University Hospital, Kuala Lumpur, Malaysia were analyzed. All the strains were susceptible to ampicillin, ceftriaxone, ciprofloxacin, chloramphenicol, tetracycline, trimethoprim, gentamicin and co-trimoxazole. Ribosomal RNA gene restriction pattern analysis of PstI-digested DNA gave three ribotypes while pulsed-field gel electrophoresis (PFGE) analysis of XbaI-digested DNA gave ten distinct profiles. PFGE was more discriminative than ribotyping in distinguishing the strains. The majority of the strains analyzed were very closely related with similarity coefficient values ranging from 0.8 to 1.0. Both PFGE and ribotyping could distinguish one of the strains which was obtained from a patient following a bone marrow transplant for β-thalassemia major, indicating that this particular strain was unrelated to the rest of the strains from patients with acute gastroenteritis. SEAMEO Regional Tropical Medicine and Public Health Network 2000 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5650/1/Application_of_ribosomal_RNA_gene_restriction_patterns_analysis_and_pulsed-field_gel_electrophoresis_in_distinguishing_Salmonella_weltevreden_isolates_in_Malaysia..pdf Goh, Yee Ling and Puthucheary, Savithri Devi and Thong, Kwai Lin (2000) Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia. The Southeast Asian Journal of Tropical Medicine and Public Health, 31 (4). pp. 697-701. ISSN 0125-1562, https://www.tm.mahidol.ac.th/seameo/2000_31_4/15-2580.pdf |
| spellingShingle | Q Science (General) QR Microbiology Goh, Yee Ling Puthucheary, Savithri Devi Thong, Kwai Lin Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia |
| title | Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia |
| title_full | Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia |
| title_fullStr | Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia |
| title_full_unstemmed | Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia |
| title_short | Application of Ribosomal RNA Gene Restriction Patterns Analysis and Pulsed-Field Gel Electrophoresis in Distinguishing Salmonella Weltevreden Isolates in Malaysia |
| title_sort | application of ribosomal rna gene restriction patterns analysis and pulsed field gel electrophoresis in distinguishing salmonella weltevreden isolates in malaysia |
| topic | Q Science (General) QR Microbiology |
| url | http://eprints.um.edu.my/5650/1/Application_of_ribosomal_RNA_gene_restriction_patterns_analysis_and_pulsed-field_gel_electrophoresis_in_distinguishing_Salmonella_weltevreden_isolates_in_Malaysia..pdf |
| work_keys_str_mv | AT gohyeeling applicationofribosomalrnagenerestrictionpatternsanalysisandpulsedfieldgelelectrophoresisindistinguishingsalmonellaweltevredenisolatesinmalaysia AT puthuchearysavithridevi applicationofribosomalrnagenerestrictionpatternsanalysisandpulsedfieldgelelectrophoresisindistinguishingsalmonellaweltevredenisolatesinmalaysia AT thongkwailin applicationofribosomalrnagenerestrictionpatternsanalysisandpulsedfieldgelelectrophoresisindistinguishingsalmonellaweltevredenisolatesinmalaysia |