A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility
The correlation of pbp 2b restriction fragment length polymorphism (RFLP) patterns of fifty clinical isolates of Malaysian Streptococcus pneumoniae, as well as the distribution of determinant genes for erythromycin resistance; mefE and ermB, with the respective minimum inhibitory concentration (MIC)...
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| Format: | Article |
| Language: | English |
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Malaysian Society for Biochemistry and Molecular Biology
2005
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| Online Access: | http://eprints.um.edu.my/5652/1/A_correlation_between_the_genes_responsible_for_penicillin_and_erythromycin_resistance_in_Streptococcus_pneumoniae_and_the_minimum_inhibitory_concentration_%28MIC%29.pdf |
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| author | Desa, Mohd Nasir Mohd Thong, Kwai Lin Navaratnam, Parasakthi |
| author_facet | Desa, Mohd Nasir Mohd Thong, Kwai Lin Navaratnam, Parasakthi |
| author_sort | Desa, Mohd Nasir Mohd |
| collection | UM |
| description | The correlation of pbp 2b restriction fragment length polymorphism (RFLP) patterns of fifty clinical isolates of Malaysian Streptococcus pneumoniae, as well as the distribution of determinant genes for erythromycin resistance; mefE and ermB, with the respective minimum inhibitory concentration (MIC) values for penicillin and erythromycin (determined by agar dilution method) were examined. Strains with penicillin MIC of ≥ 0.5 μg/ml had unique pbp 2b RFLP patterns that were absent in the penicillinsusceptible strains. The prevalence of ermB, either alone or together with mefE, was frequently observed in strains with higher MIC values (> 8 μg/ml) whereas mefE alone was observed in strains with lower MIC values (1 μg/ml - 4 μg/ml). This showed that the pbp 2b RFLP patterns and the distribution of mefE and ermB genes followed certain patterns in relation to the susceptibility. This correlation may thus be used as a diagnostic criterion for molecular detection of resistance. |
| first_indexed | 2024-03-06T05:14:54Z |
| format | Article |
| id | um.eprints-5652 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:14:54Z |
| publishDate | 2005 |
| publisher | Malaysian Society for Biochemistry and Molecular Biology |
| record_format | dspace |
| spelling | um.eprints-56522018-10-15T01:54:36Z http://eprints.um.edu.my/5652/ A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility Desa, Mohd Nasir Mohd Thong, Kwai Lin Navaratnam, Parasakthi Q Science (General) QH Natural history QR Microbiology The correlation of pbp 2b restriction fragment length polymorphism (RFLP) patterns of fifty clinical isolates of Malaysian Streptococcus pneumoniae, as well as the distribution of determinant genes for erythromycin resistance; mefE and ermB, with the respective minimum inhibitory concentration (MIC) values for penicillin and erythromycin (determined by agar dilution method) were examined. Strains with penicillin MIC of ≥ 0.5 μg/ml had unique pbp 2b RFLP patterns that were absent in the penicillinsusceptible strains. The prevalence of ermB, either alone or together with mefE, was frequently observed in strains with higher MIC values (> 8 μg/ml) whereas mefE alone was observed in strains with lower MIC values (1 μg/ml - 4 μg/ml). This showed that the pbp 2b RFLP patterns and the distribution of mefE and ermB genes followed certain patterns in relation to the susceptibility. This correlation may thus be used as a diagnostic criterion for molecular detection of resistance. Malaysian Society for Biochemistry and Molecular Biology 2005 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5652/1/A_correlation_between_the_genes_responsible_for_penicillin_and_erythromycin_resistance_in_Streptococcus_pneumoniae_and_the_minimum_inhibitory_concentration_%28MIC%29.pdf Desa, Mohd Nasir Mohd and Thong, Kwai Lin and Navaratnam, Parasakthi (2005) A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility. Malaysian Journal of Biochemistry and Molecular Biology, 12. pp. 55-57. ISSN 1511-2616, http://msbmb2010.wixsite.com/mjbmb |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Desa, Mohd Nasir Mohd Thong, Kwai Lin Navaratnam, Parasakthi A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility |
| title | A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility |
| title_full | A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility |
| title_fullStr | A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility |
| title_full_unstemmed | A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility |
| title_short | A Correlation Between the Genes Responsible for Penicillin and Erythromycin Resistance in Streptococcus Pneumoniae and the Minimum Inhibitory Concentration (MIC) Values: A Potential Approach for Molecular Detection of Susceptibility |
| title_sort | correlation between the genes responsible for penicillin and erythromycin resistance in streptococcus pneumoniae and the minimum inhibitory concentration mic values a potential approach for molecular detection of susceptibility |
| topic | Q Science (General) QH Natural history QR Microbiology |
| url | http://eprints.um.edu.my/5652/1/A_correlation_between_the_genes_responsible_for_penicillin_and_erythromycin_resistance_in_Streptococcus_pneumoniae_and_the_minimum_inhibitory_concentration_%28MIC%29.pdf |
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