Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia
The genetic diversity of 16 animal and food strains of Salmonella enterica Paratyphi B from different animal hosts between the years 1979-2001 was determined by pulsed-field gel electrophoresis (PFGE), ERIC-PCR and REP-PCR analysis. Fourteen isolates were tested positive for dextrorotary-tartrate te...
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Faculty of Science, University of Malaya
2003
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| author | Goh, Yee Ling Jamli, Maria Thong, Kwai Lin |
| author_facet | Goh, Yee Ling Jamli, Maria Thong, Kwai Lin |
| author_sort | Goh, Yee Ling |
| collection | UM |
| description | The genetic diversity of 16 animal and food strains of Salmonella enterica Paratyphi B from different animal hosts between the years 1979-2001 was determined by pulsed-field gel electrophoresis (PFGE), ERIC-PCR and REP-PCR analysis. Fourteen isolates were tested positive for dextrorotary-tartrate tests (S. Paratyphi B dT+, also known as S. Java) while two were d-tartrate negative (S. Paratyphi B dT-). Most of the strains tested were sensitive to the commonly used antibiotics. Both REP-PCR and ERIC-PCR gave five different profiles with a diversity index (DI) of 0.67 and 0.69 respectively. PFGE of XbaI digested chromosomal DNA from these isolates showed that there was a high degree of heterogeneity (F= 0.59-1.0) and most of the strains had unique profiles with a DI of 0.99. Strains that were isolated from different sources and years were genetically distinct. A pair of S. Paratyphi B dT+ strains isolated from cattle and pheasant were similar, indicating that these two different animals were probably infected with the same strain. A dendrogram based on the unweighted pair group average method (UPGMA) algorithm showed that the bovine isolates recovered from cattle in 1984 were in one cluster indicating that the strains were probably derived from a single clone. PFGE is more discriminative that PCR-based methods in determining the clonality and genetic diversity of Salmonella Paratyphi B. To the best of our knowledge, this is the first study to report the genetic variation of chromosomal S. enterica Paratyphi B var Java from animal and food in Malaysia. |
| first_indexed | 2024-03-06T05:14:54Z |
| format | Article |
| id | um.eprints-5655 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:14:54Z |
| publishDate | 2003 |
| publisher | Faculty of Science, University of Malaya |
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| spelling | um.eprints-56552018-10-15T01:28:25Z http://eprints.um.edu.my/5655/ Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia Goh, Yee Ling Jamli, Maria Thong, Kwai Lin Q Science (General) QH Natural history QR Microbiology The genetic diversity of 16 animal and food strains of Salmonella enterica Paratyphi B from different animal hosts between the years 1979-2001 was determined by pulsed-field gel electrophoresis (PFGE), ERIC-PCR and REP-PCR analysis. Fourteen isolates were tested positive for dextrorotary-tartrate tests (S. Paratyphi B dT+, also known as S. Java) while two were d-tartrate negative (S. Paratyphi B dT-). Most of the strains tested were sensitive to the commonly used antibiotics. Both REP-PCR and ERIC-PCR gave five different profiles with a diversity index (DI) of 0.67 and 0.69 respectively. PFGE of XbaI digested chromosomal DNA from these isolates showed that there was a high degree of heterogeneity (F= 0.59-1.0) and most of the strains had unique profiles with a DI of 0.99. Strains that were isolated from different sources and years were genetically distinct. A pair of S. Paratyphi B dT+ strains isolated from cattle and pheasant were similar, indicating that these two different animals were probably infected with the same strain. A dendrogram based on the unweighted pair group average method (UPGMA) algorithm showed that the bovine isolates recovered from cattle in 1984 were in one cluster indicating that the strains were probably derived from a single clone. PFGE is more discriminative that PCR-based methods in determining the clonality and genetic diversity of Salmonella Paratyphi B. To the best of our knowledge, this is the first study to report the genetic variation of chromosomal S. enterica Paratyphi B var Java from animal and food in Malaysia. Faculty of Science, University of Malaya 2003 Article PeerReviewed Goh, Yee Ling and Jamli, Maria and Thong, Kwai Lin (2003) Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia. Malaysian Journal of Science, 22 (1). pp. 15-22. ISSN 1394-3065, https://mjs.um.edu.my/index.php/MJS/article/view/8793 |
| spellingShingle | Q Science (General) QH Natural history QR Microbiology Goh, Yee Ling Jamli, Maria Thong, Kwai Lin Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia |
| title | Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia |
| title_full | Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia |
| title_fullStr | Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia |
| title_full_unstemmed | Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia |
| title_short | Molecular Subtyping of Salmonella enterica Serovar Paratyphi B from Animal and Food Isolates in Malaysia |
| title_sort | molecular subtyping of salmonella enterica serovar paratyphi b from animal and food isolates in malaysia |
| topic | Q Science (General) QH Natural history QR Microbiology |
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