Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor

A single-pixel dry-type reconstituted bacteriorhodopsin (bR) sensor (Au/CMC + bR/ITO) incorporating carboxyrnethylcellulose (CMC) as the artificial membrane is fabricated according to a protocol developed to investigate signal acquisition from the proposed biosensor. Droplets of reconstituted purple...

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Main Authors: Vengadesh, P., Majid, W.H.A., Shanmugam, S.A., Low, K.S.
Format: Article
Published: 2006
Subjects:
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author Vengadesh, P.
Majid, W.H.A.
Shanmugam, S.A.
Low, K.S.
author_facet Vengadesh, P.
Majid, W.H.A.
Shanmugam, S.A.
Low, K.S.
author_sort Vengadesh, P.
collection UM
description A single-pixel dry-type reconstituted bacteriorhodopsin (bR) sensor (Au/CMC + bR/ITO) incorporating carboxyrnethylcellulose (CMC) as the artificial membrane is fabricated according to a protocol developed to investigate signal acquisition from the proposed biosensor. Droplets of reconstituted purple membrane (PM) containing bR applied onto a gel-like solution of CMC placed on gold electrodes (anode) are allowed to dry under an electric field, utilising the PM fragment's net negative charge and electric dipole moment. The resulting bR matrix thin film with high molecular orientation is finally sandwiched together with a semi-transparent indium titanium oxide (ITO) counter electrode and proper electrical connections made to form a single pixel bR-CMC photosensor. Efficient and reproducible photoresponse observed upon illumination clearly exhibits the potential of the proposed biosensor for future photosensing works. (C) 2006 Elsevier B.V. All rights reserved.
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spelling um.eprints-57052014-10-21T03:58:31Z http://eprints.um.edu.my/5705/ Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor Vengadesh, P. Majid, W.H.A. Shanmugam, S.A. Low, K.S. QC Physics A single-pixel dry-type reconstituted bacteriorhodopsin (bR) sensor (Au/CMC + bR/ITO) incorporating carboxyrnethylcellulose (CMC) as the artificial membrane is fabricated according to a protocol developed to investigate signal acquisition from the proposed biosensor. Droplets of reconstituted purple membrane (PM) containing bR applied onto a gel-like solution of CMC placed on gold electrodes (anode) are allowed to dry under an electric field, utilising the PM fragment's net negative charge and electric dipole moment. The resulting bR matrix thin film with high molecular orientation is finally sandwiched together with a semi-transparent indium titanium oxide (ITO) counter electrode and proper electrical connections made to form a single pixel bR-CMC photosensor. Efficient and reproducible photoresponse observed upon illumination clearly exhibits the potential of the proposed biosensor for future photosensing works. (C) 2006 Elsevier B.V. All rights reserved. 2006 Article PeerReviewed Vengadesh, P. and Majid, W.H.A. and Shanmugam, S.A. and Low, K.S. (2006) Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor. Organic Electronics, 7 (5). pp. 300-304. ISSN 1566-1199, DOI https://doi.org/10.1016/j.orgel.2006.03.012 <https://doi.org/10.1016/j.orgel.2006.03.012>. 10.1016/j.orgel.2006.03.012
spellingShingle QC Physics
Vengadesh, P.
Majid, W.H.A.
Shanmugam, S.A.
Low, K.S.
Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor
title Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor
title_full Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor
title_fullStr Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor
title_full_unstemmed Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor
title_short Fabrication and Photoresponse of Novel Carboxymethylcellulose (CMC) Based Bacteriorhodopsin (bR) Sensor
title_sort fabrication and photoresponse of novel carboxymethylcellulose cmc based bacteriorhodopsin br sensor
topic QC Physics
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AT shanmugamsa fabricationandphotoresponseofnovelcarboxymethylcellulosecmcbasedbacteriorhodopsinbrsensor
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