Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli

The disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and gradient-plate techniques were implemented to evaluate the antibacterial activity of honey and propolis against Staphylococcus aureus and Escherichia coli. The growth of S. aureus was inhi...

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Main Authors: Rahman, M.M., Richardson, A., Sofian-Azirun, Mohd
Format: Article
Language:English
Published: 2010
Subjects:
Online Access:http://eprints.um.edu.my/5786/1/Antibacterial_activity_of_propolis_and_honey_against_Staphylococcus_aureus_and_Escherichia_coli.pdf
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author Rahman, M.M.
Richardson, A.
Sofian-Azirun, Mohd
author_facet Rahman, M.M.
Richardson, A.
Sofian-Azirun, Mohd
author_sort Rahman, M.M.
collection UM
description The disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and gradient-plate techniques were implemented to evaluate the antibacterial activity of honey and propolis against Staphylococcus aureus and Escherichia coli. The growth of S. aureus was inhibited by application of propolis and honey at concentrations of 2.74 to 5.48 mg ml(-1) and 375.0 at mg ml(-1), respectively at both MIC and MBC. The greater inhibition zones (13.0 +/- 0.09 to 15.0 +/- 0.11 mm) were observed from propolis at concentrations of 2.74 to 5.48 mg ml(-1) in the disc diffusion method which was closely correlated with the MIC, MBC and gradient-plate technique results. The combined results obtained from the disc diffusion test, MIC, MBC and gradient-plate techniques suggested that propolis at concentrations of 2.74 to 3.5 and 3.5 mg ml(-1) was effective to inhibit S. aureus and E. coli, respectively. On the contrary, honey was effective to inhibit S. aureus at the concentration of 375.0 mg ml(-1) but failed to inhibit E. coli growth at same concentration. The combined results from all methods indicated that both propolis and honey had antibacterial activity against S. aureus. Present findings also suggested that S. aureus is more susceptible to the effect of the propolis than its Gram-negative counterpart E. coli.
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spelling um.eprints-57862019-12-04T09:02:38Z http://eprints.um.edu.my/5786/ Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli Rahman, M.M. Richardson, A. Sofian-Azirun, Mohd QH301 Biology The disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and gradient-plate techniques were implemented to evaluate the antibacterial activity of honey and propolis against Staphylococcus aureus and Escherichia coli. The growth of S. aureus was inhibited by application of propolis and honey at concentrations of 2.74 to 5.48 mg ml(-1) and 375.0 at mg ml(-1), respectively at both MIC and MBC. The greater inhibition zones (13.0 +/- 0.09 to 15.0 +/- 0.11 mm) were observed from propolis at concentrations of 2.74 to 5.48 mg ml(-1) in the disc diffusion method which was closely correlated with the MIC, MBC and gradient-plate technique results. The combined results obtained from the disc diffusion test, MIC, MBC and gradient-plate techniques suggested that propolis at concentrations of 2.74 to 3.5 and 3.5 mg ml(-1) was effective to inhibit S. aureus and E. coli, respectively. On the contrary, honey was effective to inhibit S. aureus at the concentration of 375.0 mg ml(-1) but failed to inhibit E. coli growth at same concentration. The combined results from all methods indicated that both propolis and honey had antibacterial activity against S. aureus. Present findings also suggested that S. aureus is more susceptible to the effect of the propolis than its Gram-negative counterpart E. coli. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5786/1/Antibacterial_activity_of_propolis_and_honey_against_Staphylococcus_aureus_and_Escherichia_coli.pdf Rahman, M.M. and Richardson, A. and Sofian-Azirun, Mohd (2010) Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli. African Journal of Microbiology Research, 4 (18). pp. 1872-1878. ISSN 1996-0808,
spellingShingle QH301 Biology
Rahman, M.M.
Richardson, A.
Sofian-Azirun, Mohd
Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli
title Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli
title_full Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli
title_fullStr Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli
title_full_unstemmed Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli
title_short Antibacterial Activity of Propolis and Honey Against Staphylococcus aureus and Escherichia coli
title_sort antibacterial activity of propolis and honey against staphylococcus aureus and escherichia coli
topic QH301 Biology
url http://eprints.um.edu.my/5786/1/Antibacterial_activity_of_propolis_and_honey_against_Staphylococcus_aureus_and_Escherichia_coli.pdf
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