Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers
Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Faculty of Science, University of Malaya
2002
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/5899/1/Comparison_between_two_transformation_system_for_introducing_CMV_coat_protein_construct_into_local_hot_peppers.pdf |
| _version_ | 1825719167196594176 |
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| author | Zainuddin, Z. Khalid, Norzulaani Tan, C.S. |
| author_facet | Zainuddin, Z. Khalid, Norzulaani Tan, C.S. |
| author_sort | Zainuddin, Z. |
| collection | UM |
| description | Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin (50 mg/l). Based on PCR analysis, transformation via A. tumefaciens was the best approach for introducing a novel chimeric gene into chilli tissues. |
| first_indexed | 2024-03-06T05:15:32Z |
| format | Article |
| id | um.eprints-5899 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:15:32Z |
| publishDate | 2002 |
| publisher | Faculty of Science, University of Malaya |
| record_format | dspace |
| spelling | um.eprints-58992021-04-27T07:50:38Z http://eprints.um.edu.my/5899/ Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers Zainuddin, Z. Khalid, Norzulaani Tan, C.S. QH301 Biology Two transformation systems, via Agro bacterium tumefaciens and particle bombardment, for two varieties of chilli pepper (Capsicum annuum L.) were determined. The plasmid pCMV.CP was used for the transformation. After transformation, buds were selected on the regeneration medium containing kanamycin (50 mg/l). Based on PCR analysis, transformation via A. tumefaciens was the best approach for introducing a novel chimeric gene into chilli tissues. Faculty of Science, University of Malaya 2002 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5899/1/Comparison_between_two_transformation_system_for_introducing_CMV_coat_protein_construct_into_local_hot_peppers.pdf Zainuddin, Z. and Khalid, Norzulaani and Tan, C.S. (2002) Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers. Malaysian Journal of Science, 21 (1 & 2). pp. 25-30. ISSN 1394-3065, |
| spellingShingle | QH301 Biology Zainuddin, Z. Khalid, Norzulaani Tan, C.S. Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers |
| title | Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers |
| title_full | Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers |
| title_fullStr | Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers |
| title_full_unstemmed | Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers |
| title_short | Comparison between Two Transformation Systems for Introducing CMV Coat Protein Construct into Local Hot Peppers |
| title_sort | comparison between two transformation systems for introducing cmv coat protein construct into local hot peppers |
| topic | QH301 Biology |
| url | http://eprints.um.edu.my/5899/1/Comparison_between_two_transformation_system_for_introducing_CMV_coat_protein_construct_into_local_hot_peppers.pdf |
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