Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture
Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifu...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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2011
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| Online Access: | http://eprints.um.edu.my/5975/1/Optimization_of_two-dimensional_gel_electrophoresis_protocols_for_boesenbergia_rotunda_in_vitro_suspension_culture.pdf |
| _version_ | 1796945374065197056 |
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| author | Chong, T.E. Teck, F.G. Ming, W.S. Rahman, N.A. Khalid, N. Karsani, S.A. Othman, S. Yusof, R. |
| author_facet | Chong, T.E. Teck, F.G. Ming, W.S. Rahman, N.A. Khalid, N. Karsani, S.A. Othman, S. Yusof, R. |
| author_sort | Chong, T.E. |
| collection | UM |
| description | Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifungal, anti-inflammatory, anti-tumor and antioxidant activities. Proteomics approaches to study the proteins and/or enzymes involved in the biosynthesis of these compounds are challenging due to the complexity of plant samples and the presence of interfering substances. Here, we describe the development a highly robust and reproducible two-dimensional gel electrophoresis (2DE) protocols for resolving the proteome of B. rotunda suspension cultures. |
| first_indexed | 2024-03-06T05:15:41Z |
| format | Article |
| id | um.eprints-5975 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:15:41Z |
| publishDate | 2011 |
| record_format | dspace |
| spelling | um.eprints-59752013-05-07T02:03:57Z http://eprints.um.edu.my/5975/ Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture Chong, T.E. Teck, F.G. Ming, W.S. Rahman, N.A. Khalid, N. Karsani, S.A. Othman, S. Yusof, R. QH301 Biology Boesenbergia rotunda belongs to the Zingiberaceae family and is abundant in the Southeast Asia. It is widely used as food ingredient and traditional medicine. Biologically, the plant extract contains pharmaceutically important bioactive compounds that exhibit anti-HIV protease, antibacterial, antifungal, anti-inflammatory, anti-tumor and antioxidant activities. Proteomics approaches to study the proteins and/or enzymes involved in the biosynthesis of these compounds are challenging due to the complexity of plant samples and the presence of interfering substances. Here, we describe the development a highly robust and reproducible two-dimensional gel electrophoresis (2DE) protocols for resolving the proteome of B. rotunda suspension cultures. 2011 Article PeerReviewed application/pdf en http://eprints.um.edu.my/5975/1/Optimization_of_two-dimensional_gel_electrophoresis_protocols_for_boesenbergia_rotunda_in_vitro_suspension_culture.pdf Chong, T.E. and Teck, F.G. and Ming, W.S. and Rahman, N.A. and Khalid, N. and Karsani, S.A. and Othman, S. and Yusof, R. (2011) Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture. Journal of Medicinal Plants Research, 5 (16). pp. 3777-3780. ISSN 19960875, |
| spellingShingle | QH301 Biology Chong, T.E. Teck, F.G. Ming, W.S. Rahman, N.A. Khalid, N. Karsani, S.A. Othman, S. Yusof, R. Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| title | Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| title_full | Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| title_fullStr | Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| title_full_unstemmed | Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| title_short | Optimization of two-dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| title_sort | optimization of two dimensional gel electrophoresis protocols for boesenbergia rotunda in vitro suspension culture |
| topic | QH301 Biology |
| url | http://eprints.um.edu.my/5975/1/Optimization_of_two-dimensional_gel_electrophoresis_protocols_for_boesenbergia_rotunda_in_vitro_suspension_culture.pdf |
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