Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA)
Embryogenic callus was established using immature male flower of Musa acuminata cv. Mas. After 5-6 months of culture, embryogenic callus was obtained at 21.75±11.9 from 750 immature male flower clusters with translucent somatic embryos proliferated from the whitish friable callus. It was observed t...
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Springer
2003
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| author | Jalil, M. Khalid, N. Othman, R.Y. |
| author_facet | Jalil, M. Khalid, N. Othman, R.Y. |
| author_sort | Jalil, M. |
| collection | UM |
| description | Embryogenic callus was established using immature male flower of Musa acuminata cv. Mas. After 5-6 months of culture, embryogenic callus was obtained at 21.75±11.9 from 750 immature male flower clusters with translucent somatic embryos proliferated from the whitish friable callus. It was observed that flower clusters ranging from 4 to 11 responded to form embryogenic callus and out of which 3-10 somatic embryos were formed per flower cluster. Embryogenic callus were obtained at a percentage of 10.00±0.3 on M1 medium initially supplemented with 18 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for 3 months and subsequently transferred to the same media with reduced 2,4-D (9 μM) for the next 2-3 months. Embryos developed into translucent spheres and slightly torpedo shaped embryos in suspension cultures. Plantlets were obtained on medium M4 supplemented with 0.8 μM BA, at an average regeneration rate of 13.00±0.58. |
| first_indexed | 2024-03-06T05:15:43Z |
| format | Article |
| id | um.eprints-6002 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:15:43Z |
| publishDate | 2003 |
| publisher | Springer |
| record_format | dspace |
| spelling | um.eprints-60022019-01-14T01:58:22Z http://eprints.um.edu.my/6002/ Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) Jalil, M. Khalid, N. Othman, R.Y. QH301 Biology Embryogenic callus was established using immature male flower of Musa acuminata cv. Mas. After 5-6 months of culture, embryogenic callus was obtained at 21.75±11.9 from 750 immature male flower clusters with translucent somatic embryos proliferated from the whitish friable callus. It was observed that flower clusters ranging from 4 to 11 responded to form embryogenic callus and out of which 3-10 somatic embryos were formed per flower cluster. Embryogenic callus were obtained at a percentage of 10.00±0.3 on M1 medium initially supplemented with 18 μM 2,4-dichlorophenoxyacetic acid (2,4-D) for 3 months and subsequently transferred to the same media with reduced 2,4-D (9 μM) for the next 2-3 months. Embryos developed into translucent spheres and slightly torpedo shaped embryos in suspension cultures. Plantlets were obtained on medium M4 supplemented with 0.8 μM BA, at an average regeneration rate of 13.00±0.58. Springer 2003 Article PeerReviewed Jalil, M. and Khalid, N. and Othman, R.Y. (2003) Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA). Plant Cell, Tissue and Organ Culture, 75 (3). pp. 209-214. ISSN 0167-6857, DOI https://doi.org/10.1023/A:1025814922547 <https://doi.org/10.1023/A:1025814922547>. https://doi.org/10.1023/A:1025814922547 doi:10.1023/A:1025814922547 |
| spellingShingle | QH301 Biology Jalil, M. Khalid, N. Othman, R.Y. Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) |
| title | Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) |
| title_full | Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) |
| title_fullStr | Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) |
| title_full_unstemmed | Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) |
| title_short | Plant Regeneration from embryogenic suspension cultures of Musa acuminata cv. Mas (AA) |
| title_sort | plant regeneration from embryogenic suspension cultures of musa acuminata cv mas aa |
| topic | QH301 Biology |
| work_keys_str_mv | AT jalilm plantregenerationfromembryogenicsuspensionculturesofmusaacuminatacvmasaa AT khalidn plantregenerationfromembryogenicsuspensionculturesofmusaacuminatacvmasaa AT othmanry plantregenerationfromembryogenicsuspensionculturesofmusaacuminatacvmasaa |