Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis
Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/ K...
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| Format: | Article |
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2005
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| _version_ | 1796945378667397120 |
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| author | Tan, S.K. Pippen, R. Yusof, R. Ibrahim, H. Rahman, N. Khalid, N. |
| author_facet | Tan, S.K. Pippen, R. Yusof, R. Ibrahim, H. Rahman, N. Khalid, N. |
| author_sort | Tan, S.K. |
| collection | UM |
| description | Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/ Kaempferia pandurata (Schult), to ensure a superior and consistent supply of materials for commercialization purposes. In this study, a single-medium formulation of Murashige and Skoog (MS) supplemented with 13.54 μM 2,4-dichlorophenoxyacetic acid (2,4-D) was found to be the only medium out of eight formulations to promote the complete somatic embryogenesis process for the culture of B. rotunda (L.). Callus cultures were initiated from a total of 280 explants of rhizome meristem. The percentage of cultures forming embryogenic callus was 23.3 ± 4.3 on this MS medium augmented by 13.54 μM 2,4-D. The best plantlet regeneration rate was attained from the first subcultured callus with a mean of 6.6 ± 0.1 plantlets per 1 cm diameter aggregate of callus. Somatic embryogenesis characteristic of monocots was evident from histological studies. The regenerated plantlets have been successfully established in soil. © 2005 Society for In Vitro Biology. |
| first_indexed | 2024-03-06T05:15:45Z |
| format | Article |
| id | um.eprints-6010 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:15:45Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | um.eprints-60102014-10-20T03:42:25Z http://eprints.um.edu.my/6010/ Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis Tan, S.K. Pippen, R. Yusof, R. Ibrahim, H. Rahman, N. Khalid, N. QH301 Biology Most published protocols necessitate different media formulations for multistep somatic embryogenesis. This study aims to establish a simple but effective formulation for the regeneration of plantlets of the pharmaceutically active Boesenbergia rotunda (L.) Mansf. Kulturpfl, formerly Boesenbergia/ Kaempferia pandurata (Schult), to ensure a superior and consistent supply of materials for commercialization purposes. In this study, a single-medium formulation of Murashige and Skoog (MS) supplemented with 13.54 μM 2,4-dichlorophenoxyacetic acid (2,4-D) was found to be the only medium out of eight formulations to promote the complete somatic embryogenesis process for the culture of B. rotunda (L.). Callus cultures were initiated from a total of 280 explants of rhizome meristem. The percentage of cultures forming embryogenic callus was 23.3 ± 4.3 on this MS medium augmented by 13.54 μM 2,4-D. The best plantlet regeneration rate was attained from the first subcultured callus with a mean of 6.6 ± 0.1 plantlets per 1 cm diameter aggregate of callus. Somatic embryogenesis characteristic of monocots was evident from histological studies. The regenerated plantlets have been successfully established in soil. © 2005 Society for In Vitro Biology. 2005 Article PeerReviewed Tan, S.K. and Pippen, R. and Yusof, R. and Ibrahim, H. and Rahman, N. and Khalid, N. (2005) Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis. In Vitro Cellular and Developmental Biology - Plant, 41 (6). pp. 757-761. ISSN 10545476, DOI https://doi.org/10.1079/IVP2005695 <https://doi.org/10.1079/IVP2005695>. 10.1079/IVP2005695 |
| spellingShingle | QH301 Biology Tan, S.K. Pippen, R. Yusof, R. Ibrahim, H. Rahman, N. Khalid, N. Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis |
| title | Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis |
| title_full | Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis |
| title_fullStr | Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis |
| title_full_unstemmed | Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis |
| title_short | Simple One-Medium Formulation Regeneration of Fingerroot Boesenbergia rotunda (L.) Mansf. Kulturpfl. via Somatic Embryogenesis |
| title_sort | simple one medium formulation regeneration of fingerroot boesenbergia rotunda l mansf kulturpfl via somatic embryogenesis |
| topic | QH301 Biology |
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