Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris.
A truncated form of surface antigen 2 (SAG2) of the protozoan parasite Toxoplasma gondii was cloned and expressed in the methylotrophic yeast Pichia pastoris. This recombinant antigen, designated as recSAG2-N, contained only the N-terminal half of the native SAG2. The recSAG2-N was secreted by the P...
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| Format: | Article |
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2006
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| author | Lau, Y.L. Shamilah, H. Fong, M.Y. |
| author_facet | Lau, Y.L. Shamilah, H. Fong, M.Y. |
| author_sort | Lau, Y.L. |
| collection | UM |
| description | A truncated form of surface antigen 2 (SAG2) of the protozoan parasite Toxoplasma gondii was cloned and expressed in the methylotrophic yeast Pichia pastoris. This recombinant antigen, designated as recSAG2-N, contained only the N-terminal half of the native SAG2. The recSAG2-N was secreted by the Pichia pastoris into the culture supernatant, and it was harvested by using the trichloroacetic acid precipitation method. Specificity of recSAG2-N was evaluated in western blot assays. Fifty human serum samples, including 32 from confirmed cases of toxoplasmosis, were tested. Results from the assays showed that recSAG2-N reacted with sera from the toxoplasmosis cases only. In vivo experiments showed that serum from mice which received recSAG2-N reacted with the native SAG2 of T. gondii. |
| first_indexed | 2024-03-06T05:04:55Z |
| format | Article |
| id | um.eprints-622 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:04:55Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | um.eprints-6222014-10-21T04:37:03Z http://eprints.um.edu.my/622/ Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. Lau, Y.L. Shamilah, H. Fong, M.Y. RS Pharmacy and materia medica A truncated form of surface antigen 2 (SAG2) of the protozoan parasite Toxoplasma gondii was cloned and expressed in the methylotrophic yeast Pichia pastoris. This recombinant antigen, designated as recSAG2-N, contained only the N-terminal half of the native SAG2. The recSAG2-N was secreted by the Pichia pastoris into the culture supernatant, and it was harvested by using the trichloroacetic acid precipitation method. Specificity of recSAG2-N was evaluated in western blot assays. Fifty human serum samples, including 32 from confirmed cases of toxoplasmosis, were tested. Results from the assays showed that recSAG2-N reacted with sera from the toxoplasmosis cases only. In vivo experiments showed that serum from mice which received recSAG2-N reacted with the native SAG2 of T. gondii. 2006-12 Article PeerReviewed Lau, Y.L. and Shamilah, H. and Fong, M.Y. (2006) Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. Tropical Biomedicine, 23 (2). pp. 186-93. ISSN 0127-5720, DOI 17322821. http://www.ncbi.nlm.nih.gov/pubmed/17322821 17322821 |
| spellingShingle | RS Pharmacy and materia medica Lau, Y.L. Shamilah, H. Fong, M.Y. Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. |
| title | Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. |
| title_full | Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. |
| title_fullStr | Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. |
| title_full_unstemmed | Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. |
| title_short | Characterisation of a truncated Toxoplasma gondii surface antigen 2 (SAG2) secreted by the methylotrophic yeast Pichia pastoris. |
| title_sort | characterisation of a truncated toxoplasma gondii surface antigen 2 sag2 secreted by the methylotrophic yeast pichia pastoris |
| topic | RS Pharmacy and materia medica |
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