Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications
Surgical site infection associated with surgical instruments has always been a factor in delaying post-operative recovery of patients. The evolution in surface modification of surgical instruments can be a potential choice to overcome the nosocomial infection mainly caused by bacterial populations s...
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| Format: | Article |
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Elsevier
2020
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| author | Alias, Rodianah Mahmoodian, Reza Genasan, Krishnamurithy Vellasamy, Kumutha Malar Abd Shukor, Mohd Hamdi Kamarul, Tunku |
| author_facet | Alias, Rodianah Mahmoodian, Reza Genasan, Krishnamurithy Vellasamy, Kumutha Malar Abd Shukor, Mohd Hamdi Kamarul, Tunku |
| author_sort | Alias, Rodianah |
| collection | UM |
| description | Surgical site infection associated with surgical instruments has always been a factor in delaying post-operative recovery of patients. The evolution in surface modification of surgical instruments can be a potential choice to overcome the nosocomial infection mainly caused by bacterial populations such as Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. A study was, therefore, conducted characterising the morphology, hydrophobicity, adhesion strength, phase, Nano-hardness, surface chemistry, antimicrobial and biocompatibility of SS 316L steel deposited with a Nano-composite layer of Silver (Ag) and Tantalum oxide (Ta2O5) using physical vapour deposition magnetron sputtering. The adhesion strength of Ag/AgTa2O5 coating on SS 316L and treated at 250–850 °C of thermal treatment was evaluated using micro-scratch. The Ag/Ag–Ta2O5-400 °C was shown a 154% improvement in adhesion strength on SS 316L when compared with as-sputtered layer or Ag/Ag–Ta2O5-250, 550, 700 and 850 °C. The FESEM, XPS, and XRD indicated the segregation of Ag on the surface of SS 316L after the crystallization. Wettability and Nano-indentation tests demonstrated an increase in hydrophobicity (77.3 ± 0.3°) and Nano-hardness (1.12 ± 0.43 GPa) when compared with as-sputtered layer, after the 400 °C of thermal treatment. The antibacterial performance on Ag/Ag–Ta2O5-400 °C indicated a significant zone of inhibition to Staphylococcus aureus (A-axis: 16.33 ± 0.58 mm; B-axis: 25.67 ± 0.58 mm, p < 0.01) and Escherichia coli (A-axis: 16.33 ± 1.15 mm; B-axis: 26.00 ± 0.00 mm, p < 0.01) when compared with SS 316L or Ag/Ag–Ta2O5-700 °C, which showed no inhibition. The biocompatibility tests on Ag/Ag–Ta2O5-400 °C demonstrated an excellent in cell attachment, F-actin protein expression and proliferation/viability of bone marrow derived mesenchymal stromal on day 14 when compared with uncoated or Ag/Ag–Ta2O5-700 °C. This study shows that the Ag segregation process, hydrophobicity, adhesion strength, crystallization, and hardness progressively improved after the annealing up to 400 °C. © 2019 Elsevier B.V. |
| first_indexed | 2024-03-06T05:17:30Z |
| format | Article |
| id | um.eprints-6929 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:17:30Z |
| publishDate | 2020 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-69292021-10-01T03:40:16Z http://eprints.um.edu.my/6929/ Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications Alias, Rodianah Mahmoodian, Reza Genasan, Krishnamurithy Vellasamy, Kumutha Malar Abd Shukor, Mohd Hamdi Kamarul, Tunku R Medicine TJ Mechanical engineering and machinery Surgical site infection associated with surgical instruments has always been a factor in delaying post-operative recovery of patients. The evolution in surface modification of surgical instruments can be a potential choice to overcome the nosocomial infection mainly caused by bacterial populations such as Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli. A study was, therefore, conducted characterising the morphology, hydrophobicity, adhesion strength, phase, Nano-hardness, surface chemistry, antimicrobial and biocompatibility of SS 316L steel deposited with a Nano-composite layer of Silver (Ag) and Tantalum oxide (Ta2O5) using physical vapour deposition magnetron sputtering. The adhesion strength of Ag/AgTa2O5 coating on SS 316L and treated at 250–850 °C of thermal treatment was evaluated using micro-scratch. The Ag/Ag–Ta2O5-400 °C was shown a 154% improvement in adhesion strength on SS 316L when compared with as-sputtered layer or Ag/Ag–Ta2O5-250, 550, 700 and 850 °C. The FESEM, XPS, and XRD indicated the segregation of Ag on the surface of SS 316L after the crystallization. Wettability and Nano-indentation tests demonstrated an increase in hydrophobicity (77.3 ± 0.3°) and Nano-hardness (1.12 ± 0.43 GPa) when compared with as-sputtered layer, after the 400 °C of thermal treatment. The antibacterial performance on Ag/Ag–Ta2O5-400 °C indicated a significant zone of inhibition to Staphylococcus aureus (A-axis: 16.33 ± 0.58 mm; B-axis: 25.67 ± 0.58 mm, p < 0.01) and Escherichia coli (A-axis: 16.33 ± 1.15 mm; B-axis: 26.00 ± 0.00 mm, p < 0.01) when compared with SS 316L or Ag/Ag–Ta2O5-700 °C, which showed no inhibition. The biocompatibility tests on Ag/Ag–Ta2O5-400 °C demonstrated an excellent in cell attachment, F-actin protein expression and proliferation/viability of bone marrow derived mesenchymal stromal on day 14 when compared with uncoated or Ag/Ag–Ta2O5-700 °C. This study shows that the Ag segregation process, hydrophobicity, adhesion strength, crystallization, and hardness progressively improved after the annealing up to 400 °C. © 2019 Elsevier B.V. Elsevier 2020 Article PeerReviewed Alias, Rodianah and Mahmoodian, Reza and Genasan, Krishnamurithy and Vellasamy, Kumutha Malar and Abd Shukor, Mohd Hamdi and Kamarul, Tunku (2020) Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications. Materials Science and Engineering: C, 107. p. 110304. ISSN 0928-4931, DOI https://doi.org/10.1016/j.msec.2019.110304 <https://doi.org/10.1016/j.msec.2019.110304>. https://doi.org/10.1016/j.msec.2019.110304 doi:10.1016/j.msec.2019.110304 |
| spellingShingle | R Medicine TJ Mechanical engineering and machinery Alias, Rodianah Mahmoodian, Reza Genasan, Krishnamurithy Vellasamy, Kumutha Malar Abd Shukor, Mohd Hamdi Kamarul, Tunku Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications |
| title | Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications |
| title_full | Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications |
| title_fullStr | Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications |
| title_full_unstemmed | Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications |
| title_short | Mechanical, antibacterial, and biocompatibility mechanism of PVD grown silver–tantalum-oxide-based nanostructured thin film on stainless steel 316L for surgical applications |
| title_sort | mechanical antibacterial and biocompatibility mechanism of pvd grown silver tantalum oxide based nanostructured thin film on stainless steel 316l for surgical applications |
| topic | R Medicine TJ Mechanical engineering and machinery |
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