Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda)
M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV-visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N'-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cl...
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| Format: | Article |
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2006
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| author | Ng, C.H. Ng, S.W. Yamin, B.M. Rahman, R.N. Tan, Kong Wai Kong, C.W. Ong, H.K.A. |
| author_facet | Ng, C.H. Ng, S.W. Yamin, B.M. Rahman, R.N. Tan, Kong Wai Kong, C.W. Ong, H.K.A. |
| author_sort | Ng, C.H. |
| collection | UM |
| description | M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV-visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N'-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na(2)H(2)EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change. (C) 2006 Elsevier Ltd. All rights reserved. |
| first_indexed | 2024-03-06T05:17:33Z |
| format | Article |
| id | um.eprints-6949 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:17:33Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | um.eprints-69492019-09-27T08:58:19Z http://eprints.um.edu.my/6949/ Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) Ng, C.H. Ng, S.W. Yamin, B.M. Rahman, R.N. Tan, Kong Wai Kong, C.W. Ong, H.K.A. QD Chemistry M(edda) complexes (Cu, Co, Ni, Zn) have been characterized by UV-visible spectroscopy and ESI-MS to study the species in aqueous solution. The Co complex crystallizes as the octahedral diaqua(N,N'-ethylenediaminediacetato)cobalt(II) monohydrate and its crystal structure is reported. Cu(edda) cleaves plasmid DNA in the presence of hydrogen peroxide better than Co(edda) while the Zn and Ni analogues are inactive. Partial and complete inhibition of DNA cleavage can be effected by radical scavengers and Na(2)H(2)EDTA, respectively. The cleavage efficiency of Cu(edda) varies with the concentration of the complex, pH of the buffer and the type of buffer. The difference in nucleolytic efficiency of Cu(edda) and other complexes studied can be explained by the difference in amount of OH radicals produced, as determined by a PNDA assay. ESI-MS and CD studies on the Cu(edda) complex confirms its binding to DNA and results in a non-denaturational type of conformation change. (C) 2006 Elsevier Ltd. All rights reserved. 2006 Article PeerReviewed Ng, C.H. and Ng, S.W. and Yamin, B.M. and Rahman, R.N. and Tan, Kong Wai and Kong, C.W. and Ong, H.K.A. (2006) Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda). Polyhedron, 25 (16). pp. 3118-3126. ISSN 0277-5387, DOI https://doi.org/10.1016/j.poly.2006.05.020 <https://doi.org/10.1016/j.poly.2006.05.020>. http://ac.els-cdn.com/S0277538706002567/1-s2.0-S0277538706002567-main.pdf?_tid=c0cffc54-86e0-11e2-9bae-00000aab0f02&acdnat=1362631244_f2d792239c4546ac885de66931b0841a 10.1016/j.poly.2006.05.020 |
| spellingShingle | QD Chemistry Ng, C.H. Ng, S.W. Yamin, B.M. Rahman, R.N. Tan, Kong Wai Kong, C.W. Ong, H.K.A. Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) |
| title | Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) |
| title_full | Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) |
| title_fullStr | Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) |
| title_full_unstemmed | Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) |
| title_short | Factors affecting the nucleolytic cleavage of DNA by (N,N '-ethylenendiaminediacetato)Metal(II) complexes, M(edda). crystal structure of Co(edda) |
| title_sort | factors affecting the nucleolytic cleavage of dna by n n ethylenendiaminediacetato metal ii complexes m edda crystal structure of co edda |
| topic | QD Chemistry |
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