Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda)
The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)(6), ds(AT)(6) and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is i...
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2008
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| author | Seng, H.L. Ng, C.H. Caracelli, I. Maah, M.J. Tan, K.W. Tiekink, E.R.T. Yamin, B.M. Rahman, R.N. Ong, H.K.A. |
| author_facet | Seng, H.L. Ng, C.H. Caracelli, I. Maah, M.J. Tan, K.W. Tiekink, E.R.T. Yamin, B.M. Rahman, R.N. Ong, H.K.A. |
| author_sort | Seng, H.L. |
| collection | UM |
| description | The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)(6), ds(AT)(6) and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is intercalation and there is selectivity towards AT-sequence and stacking preference for A/A parallel or diagonal adjacent base steps in their intercalation. The nucleolytic properties of these complexes were investigated and the factors affecting the extent of cleavage were determined to be: concentration of complex, the nature of metal(11) ion, type of buffer, pH of buffer, incubation time, incubation temperature, and the presence of hydrogen peroxide or ascorbic acid as exogenous reagents. The fluorescence property of these complexes and its origin were also investigated. The crystal structure of the Zn(phen)(edda) complex is reported in which the zinc atom displays a distorted trans-N4O2 octahedral geometry; the crystal packing features double layers of complex molecules held together by extensive hydrogen bonding that inter-digitate with adjacent double layers via pi...pi interactions between 1,10-phenanthroline residues. The structure is compared with that of the recently described copper(II) analogue and, with the latter, included in molecular modeling. (C) 2008 ELsevier Inc. All rights reserved. |
| first_indexed | 2024-03-06T05:17:33Z |
| format | Article |
| id | um.eprints-6950 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:17:33Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | um.eprints-69502013-09-23T06:52:49Z http://eprints.um.edu.my/6950/ Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) Seng, H.L. Ng, C.H. Caracelli, I. Maah, M.J. Tan, K.W. Tiekink, E.R.T. Yamin, B.M. Rahman, R.N. Ong, H.K.A. QD Chemistry The binding selectivity of the M(phen)(edda) (M = Cu, Co, Ni, Zn; phen = 1,10-phenanthroline, edda = ethylenediaminediacetic acid) complexes towards ds(CG)(6), ds(AT)(6) and ds(CGCGAATTCGCG) B-form oligonucleotide duplexes were studied by CD spectroscopy and molecular modeling. The binding mode is intercalation and there is selectivity towards AT-sequence and stacking preference for A/A parallel or diagonal adjacent base steps in their intercalation. The nucleolytic properties of these complexes were investigated and the factors affecting the extent of cleavage were determined to be: concentration of complex, the nature of metal(11) ion, type of buffer, pH of buffer, incubation time, incubation temperature, and the presence of hydrogen peroxide or ascorbic acid as exogenous reagents. The fluorescence property of these complexes and its origin were also investigated. The crystal structure of the Zn(phen)(edda) complex is reported in which the zinc atom displays a distorted trans-N4O2 octahedral geometry; the crystal packing features double layers of complex molecules held together by extensive hydrogen bonding that inter-digitate with adjacent double layers via pi...pi interactions between 1,10-phenanthroline residues. The structure is compared with that of the recently described copper(II) analogue and, with the latter, included in molecular modeling. (C) 2008 ELsevier Inc. All rights reserved. 2008 Article PeerReviewed Seng, H.L. and Ng, C.H. and Caracelli, I. and Maah, M.J. and Tan, K.W. and Tiekink, E.R.T. and Yamin, B.M. and Rahman, R.N. and Ong, H.K.A. (2008) Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda). Journal of Inorganic Biochemistry, 102 (11). pp. 1997-2011. ISSN 0162-0134, DOI https://doi.org/10.1016/j.jinorgbio.2008.07.015 <https://doi.org/10.1016/j.jinorgbio.2008.07.015>. http://ac.els-cdn.com/S0162013408001803/1-s2.0-S0162013408001803-main.pdf?_tid=8d8bc3fa-86e0-11e2-a8e6-00000aab0f02&acdnat=1362631158_ce15151c933662a1b64fe1044bcbd070 10.1016/j.jinorgbio.2008.07.015 |
| spellingShingle | QD Chemistry Seng, H.L. Ng, C.H. Caracelli, I. Maah, M.J. Tan, K.W. Tiekink, E.R.T. Yamin, B.M. Rahman, R.N. Ong, H.K.A. Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) |
| title | Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) |
| title_full | Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) |
| title_fullStr | Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) |
| title_full_unstemmed | Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) |
| title_short | Factors affecting nucleolytic efficiency of some ternary metal complexes with DNA binding and recognition domains. Crystal and molecular structure of Zn(phen)(edda) |
| title_sort | factors affecting nucleolytic efficiency of some ternary metal complexes with dna binding and recognition domains crystal and molecular structure of zn phen edda |
| topic | QD Chemistry |
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