Production of fructose using recycle fixed-bed reactor and batch bioreactor

Successful development of fructose syrup products lies in high yielding glucose-isomerizing enzymes and production reactor. This work focused on determining the optimum operating conditions for batch reactor by an overall desirability function, D, and evaluation of the performance of fructose produc...

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Main Authors: Rahman, N.A., Hussain, Mohd Azlan, Jahim, J.M.
Format: Article
Published: Journal of Food Agriculture & Environment 2012
Subjects:
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author Rahman, N.A.
Hussain, Mohd Azlan
Jahim, J.M.
author_facet Rahman, N.A.
Hussain, Mohd Azlan
Jahim, J.M.
author_sort Rahman, N.A.
collection UM
description Successful development of fructose syrup products lies in high yielding glucose-isomerizing enzymes and production reactor. This work focused on determining the optimum operating conditions for batch reactor by an overall desirability function, D, and evaluation of the performance of fructose production using three different types of reactors. Production of fructose from glucose isomerisation process using commercial immobilized glucose isomerase (IGI) was conducted in two bioreactors, a batch type of stirred tank bioreactor and in a continuous mode of column bioreactor. Initial concentration of glucose in the substrate used was 30 w/v with 6 g of dry immobilized glucose isomerase (IGI). The recycle system showed 50 enhancement in production as the operating conditions, temperature, feed flow rate and pH were conscientiously monitored and compared. The percentage isomerisation for batch reactor was much higher than the fixed-bed reactor recycling system by 81.64 at pH values of 8. The optimum operating conditions for both systems with substrate flow rates of 8 ml min -1 were at 65°C and pH value of 8.
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spelling um.eprints-69852021-02-10T03:50:51Z http://eprints.um.edu.my/6985/ Production of fructose using recycle fixed-bed reactor and batch bioreactor Rahman, N.A. Hussain, Mohd Azlan Jahim, J.M. TA Engineering (General). Civil engineering (General) TP Chemical technology Successful development of fructose syrup products lies in high yielding glucose-isomerizing enzymes and production reactor. This work focused on determining the optimum operating conditions for batch reactor by an overall desirability function, D, and evaluation of the performance of fructose production using three different types of reactors. Production of fructose from glucose isomerisation process using commercial immobilized glucose isomerase (IGI) was conducted in two bioreactors, a batch type of stirred tank bioreactor and in a continuous mode of column bioreactor. Initial concentration of glucose in the substrate used was 30 w/v with 6 g of dry immobilized glucose isomerase (IGI). The recycle system showed 50 enhancement in production as the operating conditions, temperature, feed flow rate and pH were conscientiously monitored and compared. The percentage isomerisation for batch reactor was much higher than the fixed-bed reactor recycling system by 81.64 at pH values of 8. The optimum operating conditions for both systems with substrate flow rates of 8 ml min -1 were at 65°C and pH value of 8. Journal of Food Agriculture & Environment 2012 Article PeerReviewed Rahman, N.A. and Hussain, Mohd Azlan and Jahim, J.M. (2012) Production of fructose using recycle fixed-bed reactor and batch bioreactor. Journal of Food Agriculture & Environment, 10 (2). pp. 268-273. ISSN 1459-0255, http://www.scopus.com/inward/record.url?eid=2-s2.0-84862227043&partnerID=40&md5=2457765bb3a4da4b4ce419227fafdb13
spellingShingle TA Engineering (General). Civil engineering (General)
TP Chemical technology
Rahman, N.A.
Hussain, Mohd Azlan
Jahim, J.M.
Production of fructose using recycle fixed-bed reactor and batch bioreactor
title Production of fructose using recycle fixed-bed reactor and batch bioreactor
title_full Production of fructose using recycle fixed-bed reactor and batch bioreactor
title_fullStr Production of fructose using recycle fixed-bed reactor and batch bioreactor
title_full_unstemmed Production of fructose using recycle fixed-bed reactor and batch bioreactor
title_short Production of fructose using recycle fixed-bed reactor and batch bioreactor
title_sort production of fructose using recycle fixed bed reactor and batch bioreactor
topic TA Engineering (General). Civil engineering (General)
TP Chemical technology
work_keys_str_mv AT rahmanna productionoffructoseusingrecyclefixedbedreactorandbatchbioreactor
AT hussainmohdazlan productionoffructoseusingrecyclefixedbedreactorandbatchbioreactor
AT jahimjm productionoffructoseusingrecyclefixedbedreactorandbatchbioreactor