Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library

BACKGROUND: Heat shock proteins (HSPs) are known to be involved in the pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of typhoid fever. The objective of this study was to apply a phage display library to identify mimotopes of two HSPs, HSP90 and DnaK in S. Typhi....

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Main Authors: Yuen, Hawk Leong, Shamala, Devi, Thong, Kwai Lin
Format: Article
Published: Open Learning on Enteric Pathogens 2008
Subjects:
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author Yuen, Hawk Leong
Shamala, Devi
Thong, Kwai Lin
author_facet Yuen, Hawk Leong
Shamala, Devi
Thong, Kwai Lin
author_sort Yuen, Hawk Leong
collection UM
description BACKGROUND: Heat shock proteins (HSPs) are known to be involved in the pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of typhoid fever. The objective of this study was to apply a phage display library to identify mimotopes of two HSPs, HSP90 and DnaK in S. Typhi. METHODOLOGY: A 12-mer random peptide library expressed on the surface of the filamentous phage, M13, was used to select the mimotopes of two S. Typhi heat shock proteins by biopanning with monoclonal antibodies (mAbs), DnaK and HSP90. The immunogenicity of the selected peptides was determined through binding affinity with polyclonal antibodies from pooled typhoid-confirmed patients' sera and purified HSPs mAb using Western blotting and ELISA. RESULTS: Five rounds of biopanning resulted in enrichment of phage clones expressing the binding motifs TDxSTRP and FPSHYWLYPPPT, respectively. The selected peptides showed strong immunoreactivity with patients' sera. Thus, monoclonal antibodies against HSP and patient sera can select common mimotopes from the random peptide library. CONCLUSION: These findings may provide fundamental information for further studies on diagnostic application or vaccine design against this aetiologic agent of typhoid fever.
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spelling um.eprints-7272018-10-15T03:00:54Z http://eprints.um.edu.my/727/ Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library Yuen, Hawk Leong Shamala, Devi Thong, Kwai Lin Q Science (General) QH Natural history RB Pathology BACKGROUND: Heat shock proteins (HSPs) are known to be involved in the pathogenesis of Salmonella enterica serovar Typhi (S. Typhi), the causative agent of typhoid fever. The objective of this study was to apply a phage display library to identify mimotopes of two HSPs, HSP90 and DnaK in S. Typhi. METHODOLOGY: A 12-mer random peptide library expressed on the surface of the filamentous phage, M13, was used to select the mimotopes of two S. Typhi heat shock proteins by biopanning with monoclonal antibodies (mAbs), DnaK and HSP90. The immunogenicity of the selected peptides was determined through binding affinity with polyclonal antibodies from pooled typhoid-confirmed patients' sera and purified HSPs mAb using Western blotting and ELISA. RESULTS: Five rounds of biopanning resulted in enrichment of phage clones expressing the binding motifs TDxSTRP and FPSHYWLYPPPT, respectively. The selected peptides showed strong immunoreactivity with patients' sera. Thus, monoclonal antibodies against HSP and patient sera can select common mimotopes from the random peptide library. CONCLUSION: These findings may provide fundamental information for further studies on diagnostic application or vaccine design against this aetiologic agent of typhoid fever. Open Learning on Enteric Pathogens 2008 Article PeerReviewed Yuen, Hawk Leong and Shamala, Devi and Thong, Kwai Lin (2008) Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library. Journal of Infection in Developing Countries, 2 (4). pp. 313-23. ISSN 2036-6590, DOI https://doi.org/10.3855/jidc.228 <https://doi.org/10.3855/jidc.228>. https://doi.org/10.3855/jidc.228 doi:10.3855/jidc.228
spellingShingle Q Science (General)
QH Natural history
RB Pathology
Yuen, Hawk Leong
Shamala, Devi
Thong, Kwai Lin
Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library
title Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library
title_full Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library
title_fullStr Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library
title_full_unstemmed Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library
title_short Mimotopes of heat shock proteins of Salmonella enterica serovar Typhi identified from phage-displayed peptide library
title_sort mimotopes of heat shock proteins of salmonella enterica serovar typhi identified from phage displayed peptide library
topic Q Science (General)
QH Natural history
RB Pathology
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