Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme

Response surface methodology (RSM) was employed for simultaneous analysis of the effects of enzymatic treatment conditions of incubation time, incubation temperature and enzyme concentration on physical characteristics such as turbidity, clarity, viscosity, and color. In this study, a two-factor cen...

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Main Authors: Liew Abdullah, A.G., Sulaiman, N.M., Aroua, M.K., Megat Mohd Noor, M.J.
Format: Article
Published: Journal of Food Engineering 2007
Subjects:
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author Liew Abdullah, A.G.
Sulaiman, N.M.
Aroua, M.K.
Megat Mohd Noor, M.J.
author_facet Liew Abdullah, A.G.
Sulaiman, N.M.
Aroua, M.K.
Megat Mohd Noor, M.J.
author_sort Liew Abdullah, A.G.
collection UM
description Response surface methodology (RSM) was employed for simultaneous analysis of the effects of enzymatic treatment conditions of incubation time, incubation temperature and enzyme concentration on physical characteristics such as turbidity, clarity, viscosity, and color. In this study, a two-factor central composite design was used to establish the optimum conditions for the enzymatic treatment for clarification of carambola fruit juice. Carambola fruit juice was treated with pectinase enzyme at different incubation time (20-100 min), incubation temperature (30-50 °C) and enzyme concentration (0.01-0.10 v/v). These three variables were used as independent variables, whose effects on turbidity, clarity, viscosity and color were evaluated. Significant regression models describing the changes on turbidity, clarity, viscosity and color with respect to the independent variables were established with coefficient of determination, R 2, greater than 0.70. The results indicated that the enzyme concentration was the most important factor affecting the characteristics of the carambola fruit juice as it exerted a significant influence on most of the dependent variables. The recommended enzymatic treatment condition from the study was at 0.10 enzyme concentration at 30 °C for 20 min.
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spelling um.eprints-74682013-12-11T03:04:45Z http://eprints.um.edu.my/7468/ Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme Liew Abdullah, A.G. Sulaiman, N.M. Aroua, M.K. Megat Mohd Noor, M.J. TA Engineering (General). Civil engineering (General) TP Chemical technology Response surface methodology (RSM) was employed for simultaneous analysis of the effects of enzymatic treatment conditions of incubation time, incubation temperature and enzyme concentration on physical characteristics such as turbidity, clarity, viscosity, and color. In this study, a two-factor central composite design was used to establish the optimum conditions for the enzymatic treatment for clarification of carambola fruit juice. Carambola fruit juice was treated with pectinase enzyme at different incubation time (20-100 min), incubation temperature (30-50 °C) and enzyme concentration (0.01-0.10 v/v). These three variables were used as independent variables, whose effects on turbidity, clarity, viscosity and color were evaluated. Significant regression models describing the changes on turbidity, clarity, viscosity and color with respect to the independent variables were established with coefficient of determination, R 2, greater than 0.70. The results indicated that the enzyme concentration was the most important factor affecting the characteristics of the carambola fruit juice as it exerted a significant influence on most of the dependent variables. The recommended enzymatic treatment condition from the study was at 0.10 enzyme concentration at 30 °C for 20 min. Journal of Food Engineering 2007 Article PeerReviewed Liew Abdullah, A.G. and Sulaiman, N.M. and Aroua, M.K. and Megat Mohd Noor, M.J. (2007) Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme. Journal of Food Engineering, 81 (1). pp. 65-71. ISSN 0260-8774, DOI https://doi.org/10.1016/j.jfoodeng.2006.10.013 <https://doi.org/10.1016/j.jfoodeng.2006.10.013>. http://www.sciencedirect.com/science/article/pii/S0260877406006406 10.1016/j.jfoodeng.2006.10.013
spellingShingle TA Engineering (General). Civil engineering (General)
TP Chemical technology
Liew Abdullah, A.G.
Sulaiman, N.M.
Aroua, M.K.
Megat Mohd Noor, M.J.
Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
title Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
title_full Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
title_fullStr Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
title_full_unstemmed Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
title_short Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
title_sort response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme
topic TA Engineering (General). Civil engineering (General)
TP Chemical technology
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