Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry
A method has been developed for the determination of trace levels of alpha-endosulfan, beta-endosulfan, endosulfan sulfate, and endosulfan diol in rat plasma and tissue samples. Endosulfan and its metabolites in the plasma samples were extracted with solid-phase extraction Chromabond-end-capped C18...
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| Format: | Article |
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2005
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| author | Chan, M.P.L. Mohd, M.A. |
| author_facet | Chan, M.P.L. Mohd, M.A. |
| author_sort | Chan, M.P.L. |
| collection | UM |
| description | A method has been developed for the determination of trace levels of alpha-endosulfan, beta-endosulfan, endosulfan sulfate, and endosulfan diol in rat plasma and tissue samples. Endosulfan and its metabolites in the plasma samples were extracted with solid-phase extraction Chromabond-end-capped C18 cartridges and analyzed by a Shimadzu QP-5050A gas chromatograph-mass spectrometer (GCMS) with quadrupole detector in selected-ion-monitoring mode. The analysis of endosulfan and its metabolites in liver and kidney samples involved solvent extraction, Florisil solid-phase-extraction cleanup, and quantitation by GCMS. Recovery experiments for the plasma and tissue samples were conducted over concentration ranges of 10-100 ng mL(-1) and 100-1000 ng mL(-1), respectively. The method was applied to the analysis of trace levels of endosulfan and its metabolites in plasma and tissue samples collected from an animal study. Trace levels of alpha-endosulfan and beta-endosulfan in the ranges of undetectable to 3.11 mug g(-1) and undetectable to 1.19 mug g(-1), respectively, were detected in the kidney samples, whereas trace levels of endosulfan sulfate in the range of 0.02-0.22 mug g(-1) were detected in the liver samples of rats. Neither endosulfan nor its metabolites was detected in any of the plasma samples. (C) 2005 Wiley Periodicals, Inc. |
| first_indexed | 2024-03-06T05:19:08Z |
| format | Article |
| id | um.eprints-7619 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:19:08Z |
| publishDate | 2005 |
| record_format | dspace |
| spelling | um.eprints-76192013-08-05T01:38:35Z http://eprints.um.edu.my/7619/ Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry Chan, M.P.L. Mohd, M.A. R Medicine A method has been developed for the determination of trace levels of alpha-endosulfan, beta-endosulfan, endosulfan sulfate, and endosulfan diol in rat plasma and tissue samples. Endosulfan and its metabolites in the plasma samples were extracted with solid-phase extraction Chromabond-end-capped C18 cartridges and analyzed by a Shimadzu QP-5050A gas chromatograph-mass spectrometer (GCMS) with quadrupole detector in selected-ion-monitoring mode. The analysis of endosulfan and its metabolites in liver and kidney samples involved solvent extraction, Florisil solid-phase-extraction cleanup, and quantitation by GCMS. Recovery experiments for the plasma and tissue samples were conducted over concentration ranges of 10-100 ng mL(-1) and 100-1000 ng mL(-1), respectively. The method was applied to the analysis of trace levels of endosulfan and its metabolites in plasma and tissue samples collected from an animal study. Trace levels of alpha-endosulfan and beta-endosulfan in the ranges of undetectable to 3.11 mug g(-1) and undetectable to 1.19 mug g(-1), respectively, were detected in the kidney samples, whereas trace levels of endosulfan sulfate in the range of 0.02-0.22 mug g(-1) were detected in the liver samples of rats. Neither endosulfan nor its metabolites was detected in any of the plasma samples. (C) 2005 Wiley Periodicals, Inc. 2005 Article PeerReviewed Chan, M.P.L. and Mohd, M.A. (2005) Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry. Environmental Toxicology, 20 (1). pp. 45-52. ISSN 1520-4081, DOI https://doi.org/10.1002/tox.20076 <https://doi.org/10.1002/tox.20076>. http://onlinelibrary.wiley.com/doi/10.1002/tox.20076/abstract 10.1002/tox.20076 |
| spellingShingle | R Medicine Chan, M.P.L. Mohd, M.A. Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry |
| title | Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry |
| title_full | Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry |
| title_fullStr | Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry |
| title_full_unstemmed | Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry |
| title_short | Analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography-mass spectrometry |
| title_sort | analysis of endosulfan and its metabolites in rat plasma and selected tissue samples by gas chromatography mass spectrometry |
| topic | R Medicine |
| work_keys_str_mv | AT chanmpl analysisofendosulfananditsmetabolitesinratplasmaandselectedtissuesamplesbygaschromatographymassspectrometry AT mohdma analysisofendosulfananditsmetabolitesinratplasmaandselectedtissuesamplesbygaschromatographymassspectrometry |