Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11
Ninety (n = 90) imipenem-resistant Pseudomonas aeruginosa (IRPA) clinical isolates collected randomly during 2005 to 2008 from University Malaya Medical Center were assessed for the presence of different variants of metallo-β-lactamase (MBL) genes. Polymerase chain reaction (PCR) assay detected 32 (...
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Elsevier
2010
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| author | Khosravi, Yalda Tay, Sun Tee Vadivelu, Jamuna |
| author_facet | Khosravi, Yalda Tay, Sun Tee Vadivelu, Jamuna |
| author_sort | Khosravi, Yalda |
| collection | UM |
| description | Ninety (n = 90) imipenem-resistant Pseudomonas aeruginosa (IRPA) clinical isolates collected randomly during 2005 to 2008 from University Malaya Medical Center were assessed for the presence of different variants of metallo-β-lactamase (MBL) genes. Polymerase chain reaction (PCR) assay detected 32 (n = 32) MBL gene PCR-positive isolates with the presence of blaIMP gene in 14 (n = 14) and blaVIM in 18 (n = 18) isolates. Four allelic variants, blaIMP-7 (12 isolates), blaIMP-4 (2 isolates), blaVIM-2 (17 isolates), and blaVIM-11 (1 isolate), of MBL genes were identified. This study is the first report of detection of blaIMP-4, blaVIM-2, and blaVIM-11 MBL genes from IRPA clinical isolates in Malaysia. |
| first_indexed | 2024-03-06T05:05:10Z |
| format | Article |
| id | um.eprints-773 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:05:10Z |
| publishDate | 2010 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | um.eprints-7732019-11-11T03:49:56Z http://eprints.um.edu.my/773/ Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 Khosravi, Yalda Tay, Sun Tee Vadivelu, Jamuna R Medicine Ninety (n = 90) imipenem-resistant Pseudomonas aeruginosa (IRPA) clinical isolates collected randomly during 2005 to 2008 from University Malaya Medical Center were assessed for the presence of different variants of metallo-β-lactamase (MBL) genes. Polymerase chain reaction (PCR) assay detected 32 (n = 32) MBL gene PCR-positive isolates with the presence of blaIMP gene in 14 (n = 14) and blaVIM in 18 (n = 18) isolates. Four allelic variants, blaIMP-7 (12 isolates), blaIMP-4 (2 isolates), blaVIM-2 (17 isolates), and blaVIM-11 (1 isolate), of MBL genes were identified. This study is the first report of detection of blaIMP-4, blaVIM-2, and blaVIM-11 MBL genes from IRPA clinical isolates in Malaysia. Elsevier 2010 Article PeerReviewed Khosravi, Yalda and Tay, Sun Tee and Vadivelu, Jamuna (2010) Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11. Diagnostic Microbiology and Infectious Disease, 67 (3). pp. 294-296. ISSN 0732-8893, DOI https://doi.org/10.1016/j.diagmicrobio.2010.02.010 <https://doi.org/10.1016/j.diagmicrobio.2010.02.010>. https://doi.org/10.1016/j.diagmicrobio.2010.02.010 doi:10.1016/j.diagmicrobio.2010.02.010 |
| spellingShingle | R Medicine Khosravi, Yalda Tay, Sun Tee Vadivelu, Jamuna Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 |
| title | Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 |
| title_full | Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 |
| title_fullStr | Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 |
| title_full_unstemmed | Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 |
| title_short | Metallo-β-lactamase–producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11 |
| title_sort | metallo β lactamase producing imipenem resistant pseudomonas aeruginosa clinical isolates in a university teaching hospital in malaysia detection of imp 7 and first identification of imp 4 vim 2 and vim 11 |
| topic | R Medicine |
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