Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen
Cattle major histocompatibility complex (MHC) (BoLA) class I molecules are heterodimeric glycoproteins which present endogenous antigenic peptides to CD8{sup +} T lymphocytes, initiating a cellular immune response. The MHC-encoded heavy chains are highly polymorphic and, in cattle, have been charact...
| Autors principals: | , , , , , , , |
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| Format: | Article |
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1995
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| _version_ | 1825719432970764288 |
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| author | Sawhney, S.M.S. Hasima, N.N. Glass, E.J. Almurrani, S.W.K. Nichani, A.K. Spooner, R.L. Williams, J.L. Russell, G.C. |
| author_facet | Sawhney, S.M.S. Hasima, N.N. Glass, E.J. Almurrani, S.W.K. Nichani, A.K. Spooner, R.L. Williams, J.L. Russell, G.C. |
| author_sort | Sawhney, S.M.S. |
| collection | UM |
| description | Cattle major histocompatibility complex (MHC) (BoLA) class I molecules are heterodimeric glycoproteins which present endogenous antigenic peptides to CD8{sup +} T lymphocytes, initiating a cellular immune response. The MHC-encoded heavy chains are highly polymorphic and, in cattle, have been characterized mainly by using allo-antisera raised by reciprocal calf/dam immunizations. This has enabled the identification of about 50 serlogical specificities, most of which behave as alleles of a single highly polymorphic class I locus. However, evidence from biochemical and molecular biological studies suggest that more than one BoLA class I locus is expressed. These loci are apparently in linkage disequilibrium, making them difficult to distinguish by conventional methods. In order to investigate the expression and function of individual class I locus products, we are correlating BoLA class I gene sequences with the expressed products by the transfection and characterization of genomic class I clones. Shotgun transfection and expression of BoLA class I molecules has been described previously, but the genes involved were not isolated. In this paper we report the isolation, DNA sequencing, transfection, and expression of a genomic clone encoding a BoLA-A11 determinant from an animal expressing A10 and A11 serological specificities. 23 refs., 3 figs. |
| first_indexed | 2024-03-06T05:19:39Z |
| format | Article |
| id | um.eprints-7815 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:19:39Z |
| publishDate | 1995 |
| record_format | dspace |
| spelling | um.eprints-78152013-11-15T02:58:30Z http://eprints.um.edu.my/7815/ Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen Sawhney, S.M.S. Hasima, N.N. Glass, E.J. Almurrani, S.W.K. Nichani, A.K. Spooner, R.L. Williams, J.L. Russell, G.C. QH301 Biology Cattle major histocompatibility complex (MHC) (BoLA) class I molecules are heterodimeric glycoproteins which present endogenous antigenic peptides to CD8{sup +} T lymphocytes, initiating a cellular immune response. The MHC-encoded heavy chains are highly polymorphic and, in cattle, have been characterized mainly by using allo-antisera raised by reciprocal calf/dam immunizations. This has enabled the identification of about 50 serlogical specificities, most of which behave as alleles of a single highly polymorphic class I locus. However, evidence from biochemical and molecular biological studies suggest that more than one BoLA class I locus is expressed. These loci are apparently in linkage disequilibrium, making them difficult to distinguish by conventional methods. In order to investigate the expression and function of individual class I locus products, we are correlating BoLA class I gene sequences with the expressed products by the transfection and characterization of genomic class I clones. Shotgun transfection and expression of BoLA class I molecules has been described previously, but the genes involved were not isolated. In this paper we report the isolation, DNA sequencing, transfection, and expression of a genomic clone encoding a BoLA-A11 determinant from an animal expressing A10 and A11 serological specificities. 23 refs., 3 figs. 1995 Article PeerReviewed Sawhney, S.M.S. and Hasima, N.N. and Glass, E.J. and Almurrani, S.W.K. and Nichani, A.K. and Spooner, R.L. and Williams, J.L. and Russell, G.C. (1995) Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen. Immunogenetics, 41 (4). pp. 246-250. ISSN 0093-7711, |
| spellingShingle | QH301 Biology Sawhney, S.M.S. Hasima, N.N. Glass, E.J. Almurrani, S.W.K. Nichani, A.K. Spooner, R.L. Williams, J.L. Russell, G.C. Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen |
| title | Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen |
| title_full | Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen |
| title_fullStr | Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen |
| title_full_unstemmed | Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen |
| title_short | Transfection, expression, and dna-Sequence of a gene encoding a Bola-A11 antigen |
| title_sort | transfection expression and dna sequence of a gene encoding a bola a11 antigen |
| topic | QH301 Biology |
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