Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique
The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome-intact sper...
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| Format: | Article |
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2006
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| _version_ | 1825719440719740928 |
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| author | Abdullah, R.B. Hasan Adli, Durriyyah Sharifah Nooraain, H. |
| author_facet | Abdullah, R.B. Hasan Adli, Durriyyah Sharifah Nooraain, H. |
| author_sort | Abdullah, R.B. |
| collection | UM |
| description | The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely
fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin
(TRITC-LCA). The results showed that mean percentages of acrosome-intact sperm incubated in vitro were
considerably low. After one-hour incubation there were 43.20±2.83, 31.81±8.44 and 30.90±4.15% for ICR, C57/6J
and F1 sperm, respectively. Intact acrosome in ICR sperm was significantly reduced (P<0.05) to 16.95±4.23% after
two-hour incubation, however, insignificant reduction (P>0.05) was found for C57/6J and F1
sperm, which were
30.03±2.06 and 20.93±3.81%, respectively. The results showed that both dyes FITC-PSA and TRITC-LCA suitably
stained the mouse sperm and produced insignificant difference (P>0.05) in the percentages of acrosome-intact sperm,
which were 40.03±4.20 and 49.79±4.63%, respectively. One-hour incubation was adequate to capacitate sperm in
vitro. Low acrosome intact due to extended period of incubation and morphologically abnormal sperm could
compromise in vitro fertilization rates. |
| first_indexed | 2024-03-06T05:19:46Z |
| format | Article |
| id | um.eprints-7857 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:19:46Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | um.eprints-78572021-04-28T08:01:56Z http://eprints.um.edu.my/7857/ Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique Abdullah, R.B. Hasan Adli, Durriyyah Sharifah Nooraain, H. QH301 Biology The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome-intact sperm incubated in vitro were considerably low. After one-hour incubation there were 43.20±2.83, 31.81±8.44 and 30.90±4.15% for ICR, C57/6J and F1 sperm, respectively. Intact acrosome in ICR sperm was significantly reduced (P<0.05) to 16.95±4.23% after two-hour incubation, however, insignificant reduction (P>0.05) was found for C57/6J and F1 sperm, which were 30.03±2.06 and 20.93±3.81%, respectively. The results showed that both dyes FITC-PSA and TRITC-LCA suitably stained the mouse sperm and produced insignificant difference (P>0.05) in the percentages of acrosome-intact sperm, which were 40.03±4.20 and 49.79±4.63%, respectively. One-hour incubation was adequate to capacitate sperm in vitro. Low acrosome intact due to extended period of incubation and morphologically abnormal sperm could compromise in vitro fertilization rates. 2006 Article PeerReviewed Abdullah, R.B. and Hasan Adli, Durriyyah Sharifah and Nooraain, H. (2006) Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique. Malaysian Applied Biology, 35 (1). p. 43. ISSN 0126-8643, http://pkukmweb.ukm.my/msab/VOL%2035%20%20NO%201%20%20June%202006%20%20PDF/06%20%20Nooraain%20Hashim.pdf |
| spellingShingle | QH301 Biology Abdullah, R.B. Hasan Adli, Durriyyah Sharifah Nooraain, H. Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| title | Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| title_full | Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| title_fullStr | Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| title_full_unstemmed | Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| title_short | Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| title_sort | detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique |
| topic | QH301 Biology |
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