Detection of acrosome in mouse sperm incubated in vitro using fluorescence staining technique
The protocol for detecting acrosome in mouse sperm was developed by using two types of fluorescent dyes namely fluorescein conjugated Pisum sativum agglutinin (FITC-PSA) and rhodamine conjugated Lens culinaris agglutinin (TRITC-LCA). The results showed that mean percentages of acrosome-intact sper...
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Format: | Article |
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2006
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