Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy
Background: Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with diabetic retinopathy (DR). However, the mechanism on how it affects the disease development is still unclear. Aim: This study aims to investigate the relationship between 2245G/A RAGE gene pol...
| Հիմնական հեղինակներ: | , , , |
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| Ձևաչափ: | Հոդված |
| Հրապարակվել է: |
2013
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| _version_ | 1825719496106573824 |
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| author | Ng, Z.X. Kuppusamy, U.R. Iqbal, T. Chua, K.H. |
| author_facet | Ng, Z.X. Kuppusamy, U.R. Iqbal, T. Chua, K.H. |
| author_sort | Ng, Z.X. |
| collection | UM |
| description | Background: Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with diabetic retinopathy (DR). However, the mechanism on how it affects the disease development is still unclear. Aim: This study aims to investigate the relationship between 2245G/A RAGE gene polymorphism and selected pro-inflammatory, oxidative-glycation markers in DR patients. Methods: A total of 371 unrelated type 2 diabetic patients 200 with retinopathy, 171 without retinopathy (DNR) and 235 healthy subjects were recruited. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism method followed by DNA sequencing. The nuclear and cytosolic extracts from peripheral blood mononuclear cells were used for nuclear factor kappa B (NF-kappa B) p65 and superoxide dismutase activity measurement respectively. Plasma was used for glutathione peroxidase activity, advanced oxidation protein product (AOPP), monocyte chemoattractant protein (MCP)-1, pentosidine and soluble RAGE (sRAGE) measurements. Results: DR patients with 2245GA genotype had significantly elevated levels of activated NF-kappa B p65, plasma MCP-1, AOPP and pentosidine but lower level of sRAGE when compared to DR patients with wild-type 2245GG. Conclusion: The RAGE gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and circulating sRAGE in DR patients. Patients with 2245GA RAGE genotype could aggravate DR possibly via NF-kappa B mediated inflammatory pathway. (C) 2013 Elsevier B.V. All rights reserved. |
| first_indexed | 2024-03-06T05:20:38Z |
| format | Article |
| id | um.eprints-8168 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:20:38Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | um.eprints-81682013-07-23T00:41:41Z http://eprints.um.edu.my/8168/ Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy Ng, Z.X. Kuppusamy, U.R. Iqbal, T. Chua, K.H. R Medicine Background: Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with diabetic retinopathy (DR). However, the mechanism on how it affects the disease development is still unclear. Aim: This study aims to investigate the relationship between 2245G/A RAGE gene polymorphism and selected pro-inflammatory, oxidative-glycation markers in DR patients. Methods: A total of 371 unrelated type 2 diabetic patients 200 with retinopathy, 171 without retinopathy (DNR) and 235 healthy subjects were recruited. Genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism method followed by DNA sequencing. The nuclear and cytosolic extracts from peripheral blood mononuclear cells were used for nuclear factor kappa B (NF-kappa B) p65 and superoxide dismutase activity measurement respectively. Plasma was used for glutathione peroxidase activity, advanced oxidation protein product (AOPP), monocyte chemoattractant protein (MCP)-1, pentosidine and soluble RAGE (sRAGE) measurements. Results: DR patients with 2245GA genotype had significantly elevated levels of activated NF-kappa B p65, plasma MCP-1, AOPP and pentosidine but lower level of sRAGE when compared to DR patients with wild-type 2245GG. Conclusion: The RAGE gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and circulating sRAGE in DR patients. Patients with 2245GA RAGE genotype could aggravate DR possibly via NF-kappa B mediated inflammatory pathway. (C) 2013 Elsevier B.V. All rights reserved. 2013 Article PeerReviewed Ng, Z.X. and Kuppusamy, U.R. and Iqbal, T. and Chua, K.H. (2013) Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy. Gene, 521 (2). pp. 227-233. ISSN 0378-1119, DOI https://doi.org/10.1016/j.gene.2013.03.062 <https://doi.org/10.1016/j.gene.2013.03.062>. http://www.sciencedirect.com/science/article/pii/S0378111913003314 10.1016/j.gene.2013.03.062 |
| spellingShingle | R Medicine Ng, Z.X. Kuppusamy, U.R. Iqbal, T. Chua, K.H. Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy |
| title | Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy |
| title_full | Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy |
| title_fullStr | Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy |
| title_full_unstemmed | Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy |
| title_short | Receptor for advanced glycation end-product (RAGE) gene polymorphism 2245G/A is associated with pro-inflammatory, oxidative-glycation markers and sRAGE in diabetic retinopathy |
| title_sort | receptor for advanced glycation end product rage gene polymorphism 2245g a is associated with pro inflammatory oxidative glycation markers and srage in diabetic retinopathy |
| topic | R Medicine |
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