A high antioxidant level in edible plants is associated with genotoxic properties
Aqueous extracts from 20 Malaysian edible plants were screened for total phenolic content (TPC), antioxidant activity and genotoxic effects on freshly isolated human lymphocytes. Ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were per...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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2010
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| Subjects: | |
| Online Access: | http://eprints.um.edu.my/8181/1/Wan-Ibrahim-2010-A_high_antioxidant_l.pdf |
| _version_ | 1825719498215260160 |
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| author | Wan-Ibrahim, W.I. Sidik, K. Kuppusamy, U.R. |
| author_facet | Wan-Ibrahim, W.I. Sidik, K. Kuppusamy, U.R. |
| author_sort | Wan-Ibrahim, W.I. |
| collection | UM |
| description | Aqueous extracts from 20 Malaysian edible plants were screened for total phenolic content (TPC), antioxidant activity and genotoxic effects on freshly isolated human lymphocytes. Ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were performed to determine the antioxidant activity of the extracts. Single-cell gel electrophoresis (SCGE) or comet assay was carried out to determine the level of DNA damage in human lymphocytes. Of the 20 plant aqueous extracts tested, two exerted more than 50 DNA strand breaks (severe damage), nine exerted 25-50 strand breaks (moderate damage) and nine exerted <25 strand breaks (mild damage). Strong positive correlations between the extent of DNA damage and FRAP level (r = 0.816), DNA damage and TPC (r = 0.830) and DNA damage and DPPH radical scavenging activities (r = 0.859) were observed. It is evident from this study that plants rich in antioxidants have greater genotoxic effect. (C) 2010 Elsevier Ltd. All rights reserved. |
| first_indexed | 2024-03-06T05:20:40Z |
| format | Article |
| id | um.eprints-8181 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:20:40Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | um.eprints-81812013-07-24T03:00:19Z http://eprints.um.edu.my/8181/ A high antioxidant level in edible plants is associated with genotoxic properties Wan-Ibrahim, W.I. Sidik, K. Kuppusamy, U.R. R Medicine Aqueous extracts from 20 Malaysian edible plants were screened for total phenolic content (TPC), antioxidant activity and genotoxic effects on freshly isolated human lymphocytes. Ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were performed to determine the antioxidant activity of the extracts. Single-cell gel electrophoresis (SCGE) or comet assay was carried out to determine the level of DNA damage in human lymphocytes. Of the 20 plant aqueous extracts tested, two exerted more than 50 DNA strand breaks (severe damage), nine exerted 25-50 strand breaks (moderate damage) and nine exerted <25 strand breaks (mild damage). Strong positive correlations between the extent of DNA damage and FRAP level (r = 0.816), DNA damage and TPC (r = 0.830) and DNA damage and DPPH radical scavenging activities (r = 0.859) were observed. It is evident from this study that plants rich in antioxidants have greater genotoxic effect. (C) 2010 Elsevier Ltd. All rights reserved. 2010 Article PeerReviewed application/pdf en http://eprints.um.edu.my/8181/1/Wan-Ibrahim-2010-A_high_antioxidant_l.pdf Wan-Ibrahim, W.I. and Sidik, K. and Kuppusamy, U.R. (2010) A high antioxidant level in edible plants is associated with genotoxic properties. Food Chemistry, 122 (4). pp. 1139-1144. ISSN 0308-8146, DOI https://doi.org/10.1016/j.foodchem.2010.03.101 <https://doi.org/10.1016/j.foodchem.2010.03.101>. http://www.sciencedirect.com/science/article/pii/S030881461000395X 10.1016/j.foodchem.2010.03.101 |
| spellingShingle | R Medicine Wan-Ibrahim, W.I. Sidik, K. Kuppusamy, U.R. A high antioxidant level in edible plants is associated with genotoxic properties |
| title | A high antioxidant level in edible plants is associated with genotoxic properties |
| title_full | A high antioxidant level in edible plants is associated with genotoxic properties |
| title_fullStr | A high antioxidant level in edible plants is associated with genotoxic properties |
| title_full_unstemmed | A high antioxidant level in edible plants is associated with genotoxic properties |
| title_short | A high antioxidant level in edible plants is associated with genotoxic properties |
| title_sort | high antioxidant level in edible plants is associated with genotoxic properties |
| topic | R Medicine |
| url | http://eprints.um.edu.my/8181/1/Wan-Ibrahim-2010-A_high_antioxidant_l.pdf |
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