Developmental competence of transported in-vitro matured macaque oocytes
This study examines in-vitro maturation (IVM) in a non-human primate model, Macaca fascicularis. The animals had hormonal injections and laparoscopic oocyte retrieval (OR)) at 12- and 24- h after human chorionic gonadotrophin (HCG). The immature oocytes were placed in tightly capped tubes containing...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Published: |
2006
|
| Subjects: |
| _version_ | 1825719529265692672 |
|---|---|
| author | Abdullah, Ramli Ng, S.C. Chen, N. Liow, S.L. Tong, G.Q. Embong, W.K.W. Yip, W.Y. Tan, L.G. |
| author_facet | Abdullah, Ramli Ng, S.C. Chen, N. Liow, S.L. Tong, G.Q. Embong, W.K.W. Yip, W.Y. Tan, L.G. |
| author_sort | Abdullah, Ramli |
| collection | UM |
| description | This study examines in-vitro maturation (IVM) in a non-human primate model, Macaca fascicularis. The animals had hormonal injections and laparoscopic oocyte retrieval (OR)) at 12- and 24- h after human chorionic gonadotrophin (HCG). The immature oocytes were placed in tightly capped tubes containing pre-equilibrated IVM medium and transported for 5 h in a dry portable 37°C incubator without CO 2 supplement. Meiotic spindle was observed at 36-38- h post-HCG by polarized microscopy in 72 and 84.5% of mature oocytes collected at 12- and 24- h post-HCG ooeyte retrieval intervals respectively. However, abnormal spindle formations were detected in some IVM oocytes by confocal microscopy. The IVM oocytes were also randomly selected for (i) intracytoplasmic injection with frozen-thawed epididymal M. fascicularis spermatozoa and (ii) nuclear transfer (NT) with fresh M. fascicularis cumulus cells. Embryonic development of sperm-injected embryos was not affected by the 12- and 24- h post-HCG oocyte retrieval intervals (22.5 versus 27.9% respectively). However, embryonic development of NT embryos was significantly affected by the 12- h post-HCG oocyte retrieval interval (4.5 versus 31.7% respectively; P < 0.01). In conclusion, IVM of monkey oocytes in a dry portable incubator for 5 h did not affect the maturation rate. However, the ability of primate oocytes to develop after somatic cell nuclear transfer was affected by oocyte retrieval time post-HCG. |
| first_indexed | 2024-03-06T05:21:09Z |
| format | Article |
| id | um.eprints-8387 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:21:09Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | um.eprints-83872021-04-28T08:01:19Z http://eprints.um.edu.my/8387/ Developmental competence of transported in-vitro matured macaque oocytes Abdullah, Ramli Ng, S.C. Chen, N. Liow, S.L. Tong, G.Q. Embong, W.K.W. Yip, W.Y. Tan, L.G. QH301 Biology This study examines in-vitro maturation (IVM) in a non-human primate model, Macaca fascicularis. The animals had hormonal injections and laparoscopic oocyte retrieval (OR)) at 12- and 24- h after human chorionic gonadotrophin (HCG). The immature oocytes were placed in tightly capped tubes containing pre-equilibrated IVM medium and transported for 5 h in a dry portable 37°C incubator without CO 2 supplement. Meiotic spindle was observed at 36-38- h post-HCG by polarized microscopy in 72 and 84.5% of mature oocytes collected at 12- and 24- h post-HCG ooeyte retrieval intervals respectively. However, abnormal spindle formations were detected in some IVM oocytes by confocal microscopy. The IVM oocytes were also randomly selected for (i) intracytoplasmic injection with frozen-thawed epididymal M. fascicularis spermatozoa and (ii) nuclear transfer (NT) with fresh M. fascicularis cumulus cells. Embryonic development of sperm-injected embryos was not affected by the 12- and 24- h post-HCG oocyte retrieval intervals (22.5 versus 27.9% respectively). However, embryonic development of NT embryos was significantly affected by the 12- h post-HCG oocyte retrieval interval (4.5 versus 31.7% respectively; P < 0.01). In conclusion, IVM of monkey oocytes in a dry portable incubator for 5 h did not affect the maturation rate. However, the ability of primate oocytes to develop after somatic cell nuclear transfer was affected by oocyte retrieval time post-HCG. 2006 Article PeerReviewed Abdullah, Ramli and Ng, S.C. and Chen, N. and Liow, S.L. and Tong, G.Q. and Embong, W.K.W. and Yip, W.Y. and Tan, L.G. (2006) Developmental competence of transported in-vitro matured macaque oocytes. Reproductive BioMedicine Online, 12 (1). pp. 50-59. ISSN 14726483 , DOI https://doi.org/10.1093/humrep/dei283 <https://doi.org/10.1093/humrep/dei283>. http://www.sciencedirect.com/science/article/pii/S1472648310609807 10.1093/humrep/dei283 |
| spellingShingle | QH301 Biology Abdullah, Ramli Ng, S.C. Chen, N. Liow, S.L. Tong, G.Q. Embong, W.K.W. Yip, W.Y. Tan, L.G. Developmental competence of transported in-vitro matured macaque oocytes |
| title | Developmental competence of transported in-vitro matured macaque oocytes |
| title_full | Developmental competence of transported in-vitro matured macaque oocytes |
| title_fullStr | Developmental competence of transported in-vitro matured macaque oocytes |
| title_full_unstemmed | Developmental competence of transported in-vitro matured macaque oocytes |
| title_short | Developmental competence of transported in-vitro matured macaque oocytes |
| title_sort | developmental competence of transported in vitro matured macaque oocytes |
| topic | QH301 Biology |
| work_keys_str_mv | AT abdullahramli developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT ngsc developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT chenn developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT liowsl developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT tonggq developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT embongwkw developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT yipwy developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes AT tanlg developmentalcompetenceoftransportedinvitromaturedmacaqueoocytes |