Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria
Plasmodium knowlesi is now known as the fifth Plasmodium species that can cause human malaria. The Plasmodium merozoite surface protein (MSP) has been reported to be potential target for vaccination and diagnosis of malaria. MSP-1(33) has been shown to be immunogenic and its T cell epitopes could me...
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| Format: | Article |
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2013
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| author | Cheong, F.W. Lau, Y.L. Fong, M.Y. Mahmud, R. |
| author_facet | Cheong, F.W. Lau, Y.L. Fong, M.Y. Mahmud, R. |
| author_sort | Cheong, F.W. |
| collection | UM |
| description | Plasmodium knowlesi is now known as the fifth Plasmodium species that can cause human malaria. The Plasmodium merozoite surface protein (MSP) has been reported to be potential target for vaccination and diagnosis of malaria. MSP-1(33) has been shown to be immunogenic and its T cell epitopes could mediate cellular immune protection. However, limited studies have focused on P. knowlesi MSP-1(33). In this study, an approximately 28-kDa recombinant P. knowlesi MSP-1(33) (pkMSP-1(33)) was expressed by using an Escherichia coli system. The purified pkMSP-1(33) reacted with serum samples of patients infected with P. knowlesi (31 of 31, 100) and non-P. knowlesi malaria (27 of 28, 96.43) by Western blotting. The pkMSP-1(33) also reacted with P. knowlesi (25 of 31, 80.65) and non-P. knowlesi malaria sera (20 of 28, 71.43) in an enzyme-linked immunosorbent assay (ELISA). Most of the non-malarial infection (49 of 52 in by Western blotting and 46 of 52 in the ELISA) and healthy donor serum samples (65 of 65 by Western blotting and ELISA) did not react with recombinant pkMSP-1(33). |
| first_indexed | 2024-03-06T05:21:52Z |
| format | Article |
| id | um.eprints-8694 |
| institution | Universiti Malaya |
| last_indexed | 2024-03-06T05:21:52Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | um.eprints-86942013-12-16T04:35:30Z http://eprints.um.edu.my/8694/ Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria Cheong, F.W. Lau, Y.L. Fong, M.Y. Mahmud, R. R Medicine Plasmodium knowlesi is now known as the fifth Plasmodium species that can cause human malaria. The Plasmodium merozoite surface protein (MSP) has been reported to be potential target for vaccination and diagnosis of malaria. MSP-1(33) has been shown to be immunogenic and its T cell epitopes could mediate cellular immune protection. However, limited studies have focused on P. knowlesi MSP-1(33). In this study, an approximately 28-kDa recombinant P. knowlesi MSP-1(33) (pkMSP-1(33)) was expressed by using an Escherichia coli system. The purified pkMSP-1(33) reacted with serum samples of patients infected with P. knowlesi (31 of 31, 100) and non-P. knowlesi malaria (27 of 28, 96.43) by Western blotting. The pkMSP-1(33) also reacted with P. knowlesi (25 of 31, 80.65) and non-P. knowlesi malaria sera (20 of 28, 71.43) in an enzyme-linked immunosorbent assay (ELISA). Most of the non-malarial infection (49 of 52 in by Western blotting and 46 of 52 in the ELISA) and healthy donor serum samples (65 of 65 by Western blotting and ELISA) did not react with recombinant pkMSP-1(33). 2013 Article PeerReviewed Cheong, F.W. and Lau, Y.L. and Fong, M.Y. and Mahmud, R. (2013) Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria. American Journal of Tropical Medicine and Hygiene, 88 (5). pp. 835-840. ISSN 0002-9637, DOI https://doi.org/10.4269/ajtmh.12-0250 <https://doi.org/10.4269/ajtmh.12-0250>. 10.4269/ajtmh.12-0250 |
| spellingShingle | R Medicine Cheong, F.W. Lau, Y.L. Fong, M.Y. Mahmud, R. Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria |
| title | Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria |
| title_full | Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria |
| title_fullStr | Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria |
| title_full_unstemmed | Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria |
| title_short | Evaluation of recombinant plasmodium knowlesi merozoite surface Protein-1(33) for detection of human Malaria |
| title_sort | evaluation of recombinant plasmodium knowlesi merozoite surface protein 1 33 for detection of human malaria |
| topic | R Medicine |
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