Molecular characterization of Giardia duodenalis in Yemen
Giardia duodenalis is an important intestinal protozoan in Yemen with infection rates ranging from 18 to 27. To date, there has been no genotyping study to provide a better understanding of the transmission dynamic. This study was conducted to genotype and subtype G. duodenalis in Yemen. Stool sampl...
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| Format: | Article |
| Language: | English |
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2013
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| Online Access: | http://eprints.um.edu.my/8696/1/Alyousefi-2013-Molecular_characteri.pdf |
| _version_ | 1825719578227900416 |
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| author | Alyousefi, N.A. Mahdy, M.A.K. Xiao, L.H. Mahmud, R. Lim, Y.A.L. |
| author_facet | Alyousefi, N.A. Mahdy, M.A.K. Xiao, L.H. Mahmud, R. Lim, Y.A.L. |
| author_sort | Alyousefi, N.A. |
| collection | UM |
| description | Giardia duodenalis is an important intestinal protozoan in Yemen with infection rates ranging from 18 to 27. To date, there has been no genotyping study to provide a better understanding of the transmission dynamic. This study was conducted to genotype and subtype G. duodenalis in Yemen. Stool samples were collected from 503 Yemeni outpatients between I and 80 years old, including 219 males and 284 females. Giardia cysts were detected via microscopy after the formal-ether concentration. Genotyping of Giardia was carried out using PCR and sequence analysis of the 16s rRNA and b-giardin genes. Of the 89 microscopy-positive Giardia samples, 65 were successfully sequenced, of which 66 (43 of 65) were identified as G. duodenalis assemblage A and 34 (22 of 65) as assemblage B. Further subtyping analysis based on b-giardin gene identified the presence of subtypes A2 and A3, which belong to the anthroponotic sub-assemblage All. Data of the study suggest that anthroponotic transmission played a potential role in the transmission of giardiasis in the community. However, further genotyping and subtyping studies of specimens from humans and animals living in the same households are needed for a more definitive understanding of giardiasis transmission in Yemen. (C) 2013 Elsevier Inc. All rights reserved. |
| first_indexed | 2024-03-06T05:21:52Z |
| format | Article |
| id | um.eprints-8696 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:21:52Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | um.eprints-86962013-12-16T03:59:59Z http://eprints.um.edu.my/8696/ Molecular characterization of Giardia duodenalis in Yemen Alyousefi, N.A. Mahdy, M.A.K. Xiao, L.H. Mahmud, R. Lim, Y.A.L. R Medicine Giardia duodenalis is an important intestinal protozoan in Yemen with infection rates ranging from 18 to 27. To date, there has been no genotyping study to provide a better understanding of the transmission dynamic. This study was conducted to genotype and subtype G. duodenalis in Yemen. Stool samples were collected from 503 Yemeni outpatients between I and 80 years old, including 219 males and 284 females. Giardia cysts were detected via microscopy after the formal-ether concentration. Genotyping of Giardia was carried out using PCR and sequence analysis of the 16s rRNA and b-giardin genes. Of the 89 microscopy-positive Giardia samples, 65 were successfully sequenced, of which 66 (43 of 65) were identified as G. duodenalis assemblage A and 34 (22 of 65) as assemblage B. Further subtyping analysis based on b-giardin gene identified the presence of subtypes A2 and A3, which belong to the anthroponotic sub-assemblage All. Data of the study suggest that anthroponotic transmission played a potential role in the transmission of giardiasis in the community. However, further genotyping and subtyping studies of specimens from humans and animals living in the same households are needed for a more definitive understanding of giardiasis transmission in Yemen. (C) 2013 Elsevier Inc. All rights reserved. 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/8696/1/Alyousefi-2013-Molecular_characteri.pdf Alyousefi, N.A. and Mahdy, M.A.K. and Xiao, L.H. and Mahmud, R. and Lim, Y.A.L. (2013) Molecular characterization of Giardia duodenalis in Yemen. Experimental Parasitology, 134 (2). pp. 141-147. ISSN 0014-4894, DOI https://doi.org/10.1016/j.exppara.2013.03.001 <https://doi.org/10.1016/j.exppara.2013.03.001>. http://www.sciencedirect.com/science/article/pii/S0014489413000714 10.1016/j.exppara.2013.03.001 |
| spellingShingle | R Medicine Alyousefi, N.A. Mahdy, M.A.K. Xiao, L.H. Mahmud, R. Lim, Y.A.L. Molecular characterization of Giardia duodenalis in Yemen |
| title | Molecular characterization of Giardia duodenalis in Yemen |
| title_full | Molecular characterization of Giardia duodenalis in Yemen |
| title_fullStr | Molecular characterization of Giardia duodenalis in Yemen |
| title_full_unstemmed | Molecular characterization of Giardia duodenalis in Yemen |
| title_short | Molecular characterization of Giardia duodenalis in Yemen |
| title_sort | molecular characterization of giardia duodenalis in yemen |
| topic | R Medicine |
| url | http://eprints.um.edu.my/8696/1/Alyousefi-2013-Molecular_characteri.pdf |
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