Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia
Detection of Strongyloides stercoralis infection particularly in asymptomatic individuals is often hampered due to the lack of standard diagnostic tools. In this study, the use of serological and molecular approaches were investigated for the detection of S. stercoralis infection among an Orang Asli...
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| 格式: | 文件 |
| 语言: | English |
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2013
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| 在线阅读: | http://eprints.um.edu.my/8697/1/Ahmad-2013-Serological_and_mole.pdf |
| _version_ | 1825719578420838400 |
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| author | Ahmad, A.F. Hadip, F. Ngui, R. Lim, Y.A.L. Mahmud, R. |
| author_facet | Ahmad, A.F. Hadip, F. Ngui, R. Lim, Y.A.L. Mahmud, R. |
| author_sort | Ahmad, A.F. |
| collection | UM |
| description | Detection of Strongyloides stercoralis infection particularly in asymptomatic individuals is often hampered due to the lack of standard diagnostic tools. In this study, the use of serological and molecular approaches were investigated for the detection of S. stercoralis infection among an Orang Asli (indigenous) community following a preliminary detection by microscopic examination of faecal samples. Out of 54 individuals studied, 17/54 (31.5 ) were detected to be positive for S. stercoralis infection by enzyme-linked immunosorbent assay (ELISA), compared to 0/54 (0 ) by faecal examination. Further confirmation performed by a nested polymerase chain reaction (PCR) using DNA extracted from faecal samples of these 17 individuals yielded 3/17 (17.6 ) positives for S. stercoralis DNA amplification. No amplification was seen with the other 37 faecal samples, which were negative by microscopy and ELISA. As the high ELISA positive results were suspected to be false-positives, ELISA is not recommended for use as a detection tool but may be beneficial for evaluating the effectiveness of anti-Strongyloides drugs. The present finding indicated that PCR should be considered as an alternative diagnostic tool for the detection of S. stercoralis infection. |
| first_indexed | 2024-03-06T05:21:52Z |
| format | Article |
| id | um.eprints-8697 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:21:52Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | um.eprints-86972013-12-16T03:58:22Z http://eprints.um.edu.my/8697/ Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia Ahmad, A.F. Hadip, F. Ngui, R. Lim, Y.A.L. Mahmud, R. R Medicine Detection of Strongyloides stercoralis infection particularly in asymptomatic individuals is often hampered due to the lack of standard diagnostic tools. In this study, the use of serological and molecular approaches were investigated for the detection of S. stercoralis infection among an Orang Asli (indigenous) community following a preliminary detection by microscopic examination of faecal samples. Out of 54 individuals studied, 17/54 (31.5 ) were detected to be positive for S. stercoralis infection by enzyme-linked immunosorbent assay (ELISA), compared to 0/54 (0 ) by faecal examination. Further confirmation performed by a nested polymerase chain reaction (PCR) using DNA extracted from faecal samples of these 17 individuals yielded 3/17 (17.6 ) positives for S. stercoralis DNA amplification. No amplification was seen with the other 37 faecal samples, which were negative by microscopy and ELISA. As the high ELISA positive results were suspected to be false-positives, ELISA is not recommended for use as a detection tool but may be beneficial for evaluating the effectiveness of anti-Strongyloides drugs. The present finding indicated that PCR should be considered as an alternative diagnostic tool for the detection of S. stercoralis infection. 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/8697/1/Ahmad-2013-Serological_and_mole.pdf Ahmad, A.F. and Hadip, F. and Ngui, R. and Lim, Y.A.L. and Mahmud, R. (2013) Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia. Parasitology Research, 112 (8). pp. 2811-2816. ISSN 0932-0113, DOI https://doi.org/10.1007/s00436-013-3450-z <https://doi.org/10.1007/s00436-013-3450-z>. http://link.springer.com/article/10.1007/s00436-013-3450-z 10.1007/s00436-013-3450-z |
| spellingShingle | R Medicine Ahmad, A.F. Hadip, F. Ngui, R. Lim, Y.A.L. Mahmud, R. Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia |
| title | Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia |
| title_full | Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia |
| title_fullStr | Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia |
| title_full_unstemmed | Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia |
| title_short | Serological and molecular detection of Strongyloides stercoralis infection among an Orang Asli community in Malaysia |
| title_sort | serological and molecular detection of strongyloides stercoralis infection among an orang asli community in malaysia |
| topic | R Medicine |
| url | http://eprints.um.edu.my/8697/1/Ahmad-2013-Serological_and_mole.pdf |
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