Analysis and experiment of centrifugal force for microfluidic ELISA CD platform
This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD compose...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Conference or Workshop Item |
| Language: | English |
| Published: |
2010
|
| Subjects: | |
| Online Access: | http://eprints.um.edu.my/9255/1/Analysis_and_experiment_of_centrifugal_force_for_microfluidic_ELISA_CD_platform______________________.pdf |
| _version_ | 1825719675952037888 |
|---|---|
| author | Ibrahim, F. Jahanshahi, P. Abd Rahman, N. Abdul Kahar, M.K.B. Madou, M. Nozari, A.A. Soin, N. Md Dawal, S.Z. Abi Samra, K. |
| author_facet | Ibrahim, F. Jahanshahi, P. Abd Rahman, N. Abdul Kahar, M.K.B. Madou, M. Nozari, A.A. Soin, N. Md Dawal, S.Z. Abi Samra, K. |
| author_sort | Ibrahim, F. |
| collection | UM |
| description | This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs were filled with colour liquid and the fluid flow behaviour of the liquid in the CD was studied and monitored by a customized CD spin stand system equipped with visualization system. The experimental test results show an average of 9 error when compared with the theoretical calculation of burst frequency for all the reservoirs. © 2010 IEEE. |
| first_indexed | 2024-03-06T05:23:22Z |
| format | Conference or Workshop Item |
| id | um.eprints-9255 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:23:22Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | um.eprints-92552017-11-01T05:32:23Z http://eprints.um.edu.my/9255/ Analysis and experiment of centrifugal force for microfluidic ELISA CD platform Ibrahim, F. Jahanshahi, P. Abd Rahman, N. Abdul Kahar, M.K.B. Madou, M. Nozari, A.A. Soin, N. Md Dawal, S.Z. Abi Samra, K. T Technology (General) TA Engineering (General). Civil engineering (General) This paper presents an analysis and experiment of centrifugal force for microfluidic Enzyme-Linked Immunosorbent Assay (ELISA) on a compact-disc (CD) platform. The ELISA CD was designed based on the centrifugal force as a driving force while capillary force acts as preventing barrier. The CD composed of 2 layers: the substrate layer is made of PMMA and the top layer is laminated with an Adhesive Sealing Films (ASF) or thermal seal. The PMMA substrate was fabricated by CNC micromachining. The ELISA CD consists of 7 reservoirs: waste, detection, serum, conjugate, washing solution, substrate and stopping solution. In the experiment, the reservoirs were filled with colour liquid and the fluid flow behaviour of the liquid in the CD was studied and monitored by a customized CD spin stand system equipped with visualization system. The experimental test results show an average of 9 error when compared with the theoretical calculation of burst frequency for all the reservoirs. © 2010 IEEE. 2010 Conference or Workshop Item PeerReviewed application/pdf en http://eprints.um.edu.my/9255/1/Analysis_and_experiment_of_centrifugal_force_for_microfluidic_ELISA_CD_platform______________________.pdf Ibrahim, F. and Jahanshahi, P. and Abd Rahman, N. and Abdul Kahar, M.K.B. and Madou, M. and Nozari, A.A. and Soin, N. and Md Dawal, S.Z. and Abi Samra, K. (2010) Analysis and experiment of centrifugal force for microfluidic ELISA CD platform. In: 2010 IEEE EMBS Conference on Biomedical Engineering and Sciences, IECBES 2010, 2010, Kuala Lumpur. http://www.scopus.com/inward/record.url?eid=2-s2.0-79955425333&partnerID=40&md5=4d7108bfac60ee9bfcddc4e7c86efa83 ieeexplore.ieee.org/xpls/absall.jsp?arnumber=5742282 |
| spellingShingle | T Technology (General) TA Engineering (General). Civil engineering (General) Ibrahim, F. Jahanshahi, P. Abd Rahman, N. Abdul Kahar, M.K.B. Madou, M. Nozari, A.A. Soin, N. Md Dawal, S.Z. Abi Samra, K. Analysis and experiment of centrifugal force for microfluidic ELISA CD platform |
| title | Analysis and experiment of centrifugal force for microfluidic ELISA CD platform |
| title_full | Analysis and experiment of centrifugal force for microfluidic ELISA CD platform |
| title_fullStr | Analysis and experiment of centrifugal force for microfluidic ELISA CD platform |
| title_full_unstemmed | Analysis and experiment of centrifugal force for microfluidic ELISA CD platform |
| title_short | Analysis and experiment of centrifugal force for microfluidic ELISA CD platform |
| title_sort | analysis and experiment of centrifugal force for microfluidic elisa cd platform |
| topic | T Technology (General) TA Engineering (General). Civil engineering (General) |
| url | http://eprints.um.edu.my/9255/1/Analysis_and_experiment_of_centrifugal_force_for_microfluidic_ELISA_CD_platform______________________.pdf |
| work_keys_str_mv | AT ibrahimf analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT jahanshahip analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT abdrahmann analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT abdulkaharmkb analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT madoum analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT nozariaa analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT soinn analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT mddawalsz analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform AT abisamrak analysisandexperimentofcentrifugalforceformicrofluidicelisacdplatform |