Optimization of extraction parameters by using response surface methodology, purification, and identification of anthocyanin pigments in melastoma malabathricum fruit
Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell. Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that ca...
| मुख्य लेखकों: | , , , , |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
Hindawi Publishing Corporation
2013
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| विषय: | |
| ऑनलाइन पहुंच: | http://eprints.um.edu.my/9620/1/00010566_96963.pdf |
| _version_ | 1825719736154980352 |
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| author | Anuar, Nordiyanah Mohd Adnan, Ahmad Faris Saat, Naziz Aziz, Norkasmani Taha, Rosna Mat |
| author_facet | Anuar, Nordiyanah Mohd Adnan, Ahmad Faris Saat, Naziz Aziz, Norkasmani Taha, Rosna Mat |
| author_sort | Anuar, Nordiyanah |
| collection | UM |
| description | Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell. Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that can be recovered from Melastoma malabathricum fruit. Factors such as extraction temperature, extraction time, and solid to liquid ratio were identified to be significantly affecting anthocyanin extraction efficiency. By using three-level three-factor Box-Behnken design, the optimized conditions for anthocyanin extraction by acidified methanol (R (2) = 0.972) were temperature of 60°C, time of 86.82 min, and 0.5 : 35 (g/mL) solid to liquid ratio while the optimum extraction conditions by acidified ethanol (R (2) = 0.954) were temperature of 60°C, time of 120 min, and 0.5 : 23.06 (g/mL) solid to liquid ratio. The crude anthocyanin extract was further purified by using Amberlite XAD-7 and Sephadex LH-20 column chromatography. Identification of anthocyanins revealed the presence of cyanidin dihexoside, cyanidin hexoside, and delphinidin hexoside as the main anthocyanins in M. malabathricum fruit. |
| first_indexed | 2024-03-06T05:24:18Z |
| format | Article |
| id | um.eprints-9620 |
| institution | Universiti Malaya |
| language | English |
| last_indexed | 2024-03-06T05:24:18Z |
| publishDate | 2013 |
| publisher | Hindawi Publishing Corporation |
| record_format | dspace |
| spelling | um.eprints-96202021-02-09T06:36:01Z http://eprints.um.edu.my/9620/ Optimization of extraction parameters by using response surface methodology, purification, and identification of anthocyanin pigments in melastoma malabathricum fruit Anuar, Nordiyanah Mohd Adnan, Ahmad Faris Saat, Naziz Aziz, Norkasmani Taha, Rosna Mat Q Science (General) QC Physics QH Natural history Anthocyanins not just have various benefits in food industry but also have been used as natural colourants in cosmetic, coating products and as potential natural photosensitizers in solar cell. Thus, the main purpose of this study was to obtain information on the maximum yield of anthocyanin that can be recovered from Melastoma malabathricum fruit. Factors such as extraction temperature, extraction time, and solid to liquid ratio were identified to be significantly affecting anthocyanin extraction efficiency. By using three-level three-factor Box-Behnken design, the optimized conditions for anthocyanin extraction by acidified methanol (R (2) = 0.972) were temperature of 60°C, time of 86.82 min, and 0.5 : 35 (g/mL) solid to liquid ratio while the optimum extraction conditions by acidified ethanol (R (2) = 0.954) were temperature of 60°C, time of 120 min, and 0.5 : 23.06 (g/mL) solid to liquid ratio. The crude anthocyanin extract was further purified by using Amberlite XAD-7 and Sephadex LH-20 column chromatography. Identification of anthocyanins revealed the presence of cyanidin dihexoside, cyanidin hexoside, and delphinidin hexoside as the main anthocyanins in M. malabathricum fruit. Hindawi Publishing Corporation 2013 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9620/1/00010566_96963.pdf Anuar, Nordiyanah and Mohd Adnan, Ahmad Faris and Saat, Naziz and Aziz, Norkasmani and Taha, Rosna Mat (2013) Optimization of extraction parameters by using response surface methodology, purification, and identification of anthocyanin pigments in melastoma malabathricum fruit. Scientific World Journal, 2013. p. 810547. ISSN 2356-6140, DOI https://doi.org/10.1155/2013/810547 <https://doi.org/10.1155/2013/810547>. http://dx.doi.org/10.1155/2013/810547 doi:10.1155/2013/810547 |
| spellingShingle | Q Science (General) QC Physics QH Natural history Anuar, Nordiyanah Mohd Adnan, Ahmad Faris Saat, Naziz Aziz, Norkasmani Taha, Rosna Mat Optimization of extraction parameters by using response surface methodology, purification, and identification of anthocyanin pigments in melastoma malabathricum fruit |
| title | Optimization of extraction parameters by using response
surface methodology, purification, and identification of
anthocyanin pigments in melastoma malabathricum fruit |
| title_full | Optimization of extraction parameters by using response
surface methodology, purification, and identification of
anthocyanin pigments in melastoma malabathricum fruit |
| title_fullStr | Optimization of extraction parameters by using response
surface methodology, purification, and identification of
anthocyanin pigments in melastoma malabathricum fruit |
| title_full_unstemmed | Optimization of extraction parameters by using response
surface methodology, purification, and identification of
anthocyanin pigments in melastoma malabathricum fruit |
| title_short | Optimization of extraction parameters by using response
surface methodology, purification, and identification of
anthocyanin pigments in melastoma malabathricum fruit |
| title_sort | optimization of extraction parameters by using response surface methodology purification and identification of anthocyanin pigments in melastoma malabathricum fruit |
| topic | Q Science (General) QC Physics QH Natural history |
| url | http://eprints.um.edu.my/9620/1/00010566_96963.pdf |
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