Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells

Porous three-dimensional hydroxyapatite (HA) scaffolds were prepared using bovine cortical bone derived HA (BDHA). Analyses of the morphology, chemical composition, and phase purity of the scaffold were performed using scanning electron microscopy (SEM), micro-computer tomography (micro-CT), Fourier...

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Main Authors: Krishnamurithy, G., Murali, M.R., Hamdi, M., Abbas, A.A., Raghavendran, H.B., Kamarul, Tunku
Format: Article
Language:English
Published: Elsevier 2014
Subjects:
Online Access:http://eprints.um.edu.my/9868/1/Krishnamurithy-2014-Characterization_of.pdf
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author Krishnamurithy, G.
Murali, M.R.
Hamdi, M.
Abbas, A.A.
Raghavendran, H.B.
Kamarul, Tunku
author_facet Krishnamurithy, G.
Murali, M.R.
Hamdi, M.
Abbas, A.A.
Raghavendran, H.B.
Kamarul, Tunku
author_sort Krishnamurithy, G.
collection UM
description Porous three-dimensional hydroxyapatite (HA) scaffolds were prepared using bovine cortical bone derived HA (BDHA). Analyses of the morphology, chemical composition, and phase purity of the scaffold were performed using scanning electron microscopy (SEM), micro-computer tomography (micro-CT), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). SEM images revealed the rough and porous surface of the scaffold, while micro-CT showed the average porous volume of 76.7 +/- 0.6 and pore size of 0.04-0.25 mm. Single phase corresponding to standard HA was observed using XRD, and FTIR confirmed the presence of functional groups similar to HA. The EDX analysis revealed a Ca/P ratio of 1.61, which was comparable with HA stoichiometry. Compressive strength of the BDHA scaffold was found to be 1.3 +/- 0.09 MPa. After 14 days of human bone marrow stromal cells (hBMSCs) seeding, SEM and confocal analysis revealed cell attachment to the surface and infiltration into the pores. Alamar blue and alkaline phosphatase assays showed significantly increased cell proliferation and differentiation in the BDHA scaffold, when compared with that in the monolayer (p < 0.01). In addition, quantitative real-time polymerase chain reaction (qPCR) data confirmed the up regulation of genes involved in osteogenic differentiation of mesenchymal stem cells. Our findings indicate that BDHA scaffold provides a favorable physiological environment for enhanced cell attachment, proliferation, and osteogenic differentiation of hBMSCs. (C) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved.
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spelling um.eprints-98682018-10-10T09:10:14Z http://eprints.um.edu.my/9868/ Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells Krishnamurithy, G. Murali, M.R. Hamdi, M. Abbas, A.A. Raghavendran, H.B. Kamarul, Tunku R Medicine Porous three-dimensional hydroxyapatite (HA) scaffolds were prepared using bovine cortical bone derived HA (BDHA). Analyses of the morphology, chemical composition, and phase purity of the scaffold were performed using scanning electron microscopy (SEM), micro-computer tomography (micro-CT), Fourier transform infrared spectroscopy (FTIR), energy dispersive X-ray spectroscopy (EDX), and X-ray diffraction (XRD). SEM images revealed the rough and porous surface of the scaffold, while micro-CT showed the average porous volume of 76.7 +/- 0.6 and pore size of 0.04-0.25 mm. Single phase corresponding to standard HA was observed using XRD, and FTIR confirmed the presence of functional groups similar to HA. The EDX analysis revealed a Ca/P ratio of 1.61, which was comparable with HA stoichiometry. Compressive strength of the BDHA scaffold was found to be 1.3 +/- 0.09 MPa. After 14 days of human bone marrow stromal cells (hBMSCs) seeding, SEM and confocal analysis revealed cell attachment to the surface and infiltration into the pores. Alamar blue and alkaline phosphatase assays showed significantly increased cell proliferation and differentiation in the BDHA scaffold, when compared with that in the monolayer (p < 0.01). In addition, quantitative real-time polymerase chain reaction (qPCR) data confirmed the up regulation of genes involved in osteogenic differentiation of mesenchymal stem cells. Our findings indicate that BDHA scaffold provides a favorable physiological environment for enhanced cell attachment, proliferation, and osteogenic differentiation of hBMSCs. (C) 2013 Elsevier Ltd and Techna Group S.r.l. All rights reserved. Elsevier 2014 Article PeerReviewed application/pdf en http://eprints.um.edu.my/9868/1/Krishnamurithy-2014-Characterization_of.pdf Krishnamurithy, G. and Murali, M.R. and Hamdi, M. and Abbas, A.A. and Raghavendran, H.B. and Kamarul, Tunku (2014) Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells. Ceramics International, 40 (1). pp. 771-777. ISSN 0272-8842, DOI https://doi.org/10.1016/j.ceramint.2013.06.067 <https://doi.org/10.1016/j.ceramint.2013.06.067>. https://doi.org/10.1016/j.ceramint.2013.06.067 doi:10.1016/j.ceramint.2013.06.067
spellingShingle R Medicine
Krishnamurithy, G.
Murali, M.R.
Hamdi, M.
Abbas, A.A.
Raghavendran, H.B.
Kamarul, Tunku
Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
title Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
title_full Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
title_fullStr Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
title_full_unstemmed Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
title_short Characterization of bovine-derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
title_sort characterization of bovine derived porous hydroxyapatite scaffold and its potential to support osteogenic differentiation of human bone marrow derived mesenchymal stem cells
topic R Medicine
url http://eprints.um.edu.my/9868/1/Krishnamurithy-2014-Characterization_of.pdf
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