Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode
Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the...
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| Format: | Article |
| Language: | English English |
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Springer Nature
2012
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| Online Access: | https://eprints.ums.edu.my/id/eprint/1752/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/1752/2/FULLTEXT.pdf |
| _version_ | 1796909554417532928 |
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| author | Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar |
| author_facet | Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar |
| author_sort | Shafiquzzaman Siddiquee |
| collection | UMS |
| description | Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the [EMIM][Otf]/ZnO/CHIT/AuE for detection of DNA hybridization were studied. Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of 1.0 × 10-18 to 1.82 × 10-4 mol L-1, and with the detection limit of 1.0 × 10-19 mol L-1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification of Trichoderma harzianum. |
| first_indexed | 2024-04-21T02:28:10Z |
| format | Article |
| id | ums.eprints-1752 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-04-21T02:28:10Z |
| publishDate | 2012 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | ums.eprints-17522023-06-30T01:19:30Z https://eprints.ums.edu.my/id/eprint/1752/ Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar Q1-390 Science (General) QK474.8-495 Spermatophyta. Phanerogams Electrochemical DNA biosensor was successfully developed by depositing the ionic liquid (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), ZnO nanoparticles, and chitosan (CHIT) nanocomposite membrane on a modified gold electrode (AuE). The electrochemical properties of the [EMIM][Otf]/ZnO/CHIT/AuE for detection of DNA hybridization were studied. Under optimal conditions using cyclic voltammetry, the target DNA sequences could be detected in the concentration range of 1.0 × 10-18 to 1.82 × 10-4 mol L-1, and with the detection limit of 1.0 × 10-19 mol L-1. This DNA biosensor detection approaches provide a quick, sensitive, and convenient method to be used in the identification of Trichoderma harzianum. Springer Nature 2012-01-23 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/1752/1/ABSTRACT.pdf text en https://eprints.ums.edu.my/id/eprint/1752/2/FULLTEXT.pdf Shafiquzzaman Siddiquee and Nor Azah Yusof and Abu Bakar Salleh and Tan, Soon Guan and Fatimah Abu Bakar (2012) Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode. Journal of Solid State Electrochemistry, 16. pp. 273-282. ISSN 1432-8488 (In Press) http://dx.doi.org/10.1007/s10008-011-1322-y 10.1007/s10008-011-1322-y https://doi.org/10.1007/s10008-011-1322-y |
| spellingShingle | Q1-390 Science (General) QK474.8-495 Spermatophyta. Phanerogams Shafiquzzaman Siddiquee Nor Azah Yusof Abu Bakar Salleh Tan, Soon Guan Fatimah Abu Bakar Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
| title | Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
| title_full | Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
| title_fullStr | Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
| title_full_unstemmed | Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
| title_short | Development of electrochemical DNA biosensor for Trichoderma harzianum based on ionic liquid/ZnO nanoparticles/chitosan/gold electrode |
| title_sort | development of electrochemical dna biosensor for trichoderma harzianum based on ionic liquid zno nanoparticles chitosan gold electrode |
| topic | Q1-390 Science (General) QK474.8-495 Spermatophyta. Phanerogams |
| url | https://eprints.ums.edu.my/id/eprint/1752/1/ABSTRACT.pdf https://eprints.ums.edu.my/id/eprint/1752/2/FULLTEXT.pdf |
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