| Summary: | Many conventional methods available that are used for determination of formaldehyde
in fish samples, are complicated, time-consuming and costly. In this study, we develop
an electrochemical nanosensor based on the modification of a glassy carbon electrode
with chitosan, calcium oxide nanoparticles, ionic liquid, formaldehyde dehydrogenase
enzyme and cofactor NAD+ for detection of formaldehyde in Scomberomorus
commerson fish sample. Analysis was done using the cyclic voltammetry under
optimized condition (0.1 V/S scan rate, pH 7.0 and 5 seconds). Methylene blue that
was used as a redox indicator, was found to have significantly higher cathodic peak (I
= -1.08 x 10⁻⁶ A) than without methylene blue (I = -0.89 x 10⁻⁶ A). Reproducibility,
repeatability, storage stability and recovery assay in the fish sample were studied.
Reproducibility of the electrode is good with RSD from 1.73 to 2.58 % while
repeatability of the electrode is high with RSD from 5.04 to 6.30 %. Storage in the
freezer did not significantly reduce (F=1.596, p=0.172) the concentration of
formaldehyde in fish. Recovery of formaldehyde from Scomberomorus commerson fish
sample spiked with 8 ppm formaldehyde was 8.79 %. Data was analysed using one way
ANOVA. The formaldehyde nanosensor developed is a very simple and fast assay
for analysing formaldehyde levels in fish samples. The findings of this study allows
further optimization of formaldehyde nanosensor and also to analyse samples obtained
from fish samples in the wet market.
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