A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity
Observations on the effects of copper on the liver proteome of Puntius javanicus based on the one dimensional PAGE was carried out. The liver was dissected from each fish, which was separately treated with different concentrations of copper sulfate ranging from 0.1 to 5.0 mg/L. The livers were extr...
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| Format: | Article |
| Language: | English English |
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hibiscus publisher
2015
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| Online Access: | https://eprints.ums.edu.my/id/eprint/18786/1/A%20one%20dimensional%20PAGE%20.pdf https://eprints.ums.edu.my/id/eprint/18786/7/A%20one%20dimensional%20PAGE%20studies%20on%20puntius%20javanicus%20liver%20proteome%20affected%20by%20copper%20toxicity.pdf |
| _version_ | 1796909746585862144 |
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| author | Mohd Khalizan Sabullah Fisal Ahmad Azlan Jualang Gansau Mohd Rosni Sulaiman |
| author_facet | Mohd Khalizan Sabullah Fisal Ahmad Azlan Jualang Gansau Mohd Rosni Sulaiman |
| author_sort | Mohd Khalizan Sabullah |
| collection | UMS |
| description | Observations on the effects of copper on the liver proteome of Puntius javanicus based on the one dimensional PAGE was carried out. The liver was dissected from each fish, which was separately treated with different concentrations of copper sulfate ranging from 0.1 to 5.0 mg/L. The livers were extracted and one dimensional PAGE was performed under nonreducing (native) and reducing (SDS)-PAGE. Several bands were resolved in the native PAGE with probable candidates for the effect of copper observed showing an increased in the expression and down regulation strongly associated with increasing copper concentrations. This study showed that high concentrations of copper significantly alters P. Javanicus liver at the proteome level, and preliminary screening based on one dimensional PAGE is considered rapid and simple to assess the toxicity effect of copper before more advanced and extensive assessment with a second dimensional PAGE is carried out. |
| first_indexed | 2024-03-06T02:54:10Z |
| format | Article |
| id | ums.eprints-18786 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T02:54:10Z |
| publishDate | 2015 |
| publisher | hibiscus publisher |
| record_format | dspace |
| spelling | ums.eprints-187862020-12-09T02:26:42Z https://eprints.ums.edu.my/id/eprint/18786/ A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity Mohd Khalizan Sabullah Fisal Ahmad Azlan Jualang Gansau Mohd Rosni Sulaiman SH Aquaculture. Fisheries. Angling Observations on the effects of copper on the liver proteome of Puntius javanicus based on the one dimensional PAGE was carried out. The liver was dissected from each fish, which was separately treated with different concentrations of copper sulfate ranging from 0.1 to 5.0 mg/L. The livers were extracted and one dimensional PAGE was performed under nonreducing (native) and reducing (SDS)-PAGE. Several bands were resolved in the native PAGE with probable candidates for the effect of copper observed showing an increased in the expression and down regulation strongly associated with increasing copper concentrations. This study showed that high concentrations of copper significantly alters P. Javanicus liver at the proteome level, and preliminary screening based on one dimensional PAGE is considered rapid and simple to assess the toxicity effect of copper before more advanced and extensive assessment with a second dimensional PAGE is carried out. hibiscus publisher 2015-12 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/18786/1/A%20one%20dimensional%20PAGE%20.pdf text en https://eprints.ums.edu.my/id/eprint/18786/7/A%20one%20dimensional%20PAGE%20studies%20on%20puntius%20javanicus%20liver%20proteome%20affected%20by%20copper%20toxicity.pdf Mohd Khalizan Sabullah and Fisal Ahmad and Azlan Jualang Gansau and Mohd Rosni Sulaiman (2015) A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity. Bioremediation Science and Technology Research, 3 (2). pp. 26-29. ISSN 2289-5892 http://journal.hibiscuspublisher.com/index.php/BSTR/article/view/297 |
| spellingShingle | SH Aquaculture. Fisheries. Angling Mohd Khalizan Sabullah Fisal Ahmad Azlan Jualang Gansau Mohd Rosni Sulaiman A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity |
| title | A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity |
| title_full | A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity |
| title_fullStr | A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity |
| title_full_unstemmed | A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity |
| title_short | A one dimensional PAGE studies on puntius javanicus liver proteome affected by copper toxicity |
| title_sort | one dimensional page studies on puntius javanicus liver proteome affected by copper toxicity |
| topic | SH Aquaculture. Fisheries. Angling |
| url | https://eprints.ums.edu.my/id/eprint/18786/1/A%20one%20dimensional%20PAGE%20.pdf https://eprints.ums.edu.my/id/eprint/18786/7/A%20one%20dimensional%20PAGE%20studies%20on%20puntius%20javanicus%20liver%20proteome%20affected%20by%20copper%20toxicity.pdf |
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