Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation

In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endoto...

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Main Authors: Clarence M. Ongkudon, Michael K. Danquah
Format: Article
Language:English
Published: Taylor & Francis Group 2014
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/18874/1/Analysis%20of%20endotoxins.pdf
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author Clarence M. Ongkudon
Michael K. Danquah
author_facet Clarence M. Ongkudon
Michael K. Danquah
author_sort Clarence M. Ongkudon
collection UMS
description In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endotoxin assay. This work concludes that Zn2+ (as ZnSO4) optimally binds to and increases the hydrodynamic size of endotoxins compared to Mg2+ and Ca2+. A low polydispersity index value measured after cation addition indicates the presence of homogenously suspended supramolecular endotoxin particles which calls for a centrifugal separation step for complete endotoxin removal.
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spelling ums.eprints-188742018-02-18T11:57:30Z https://eprints.ums.edu.my/id/eprint/18874/ Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation Clarence M. Ongkudon Michael K. Danquah Q Science (General) In order to understand the role of divalent metal cations particularly Zn2+, Mg2+, and Ca2+ in endotoxin removal from clarified cell lysate solutions, the aggregation of endotoxins by these cations was explored in this work using dynamic light scattering, zeta potential analysis, and improved endotoxin assay. This work concludes that Zn2+ (as ZnSO4) optimally binds to and increases the hydrodynamic size of endotoxins compared to Mg2+ and Ca2+. A low polydispersity index value measured after cation addition indicates the presence of homogenously suspended supramolecular endotoxin particles which calls for a centrifugal separation step for complete endotoxin removal. Taylor & Francis Group 2014 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/18874/1/Analysis%20of%20endotoxins.pdf Clarence M. Ongkudon and Michael K. Danquah (2014) Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation. Separation Science and Technology, 49 (1). pp. 39-46. https://doi.org/10.1080/01496395.2013.835825
spellingShingle Q Science (General)
Clarence M. Ongkudon
Michael K. Danquah
Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation
title Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation
title_full Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation
title_fullStr Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation
title_full_unstemmed Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation
title_short Analysis of endotoxins removal from clarified cell lysates by divalent metal cations-induced aggregation
title_sort analysis of endotoxins removal from clarified cell lysates by divalent metal cations induced aggregation
topic Q Science (General)
url https://eprints.ums.edu.my/id/eprint/18874/1/Analysis%20of%20endotoxins.pdf
work_keys_str_mv AT clarencemongkudon analysisofendotoxinsremovalfromclarifiedcelllysatesbydivalentmetalcationsinducedaggregation
AT michaelkdanquah analysisofendotoxinsremovalfromclarifiedcelllysatesbydivalentmetalcationsinducedaggregation