Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques
Background Plasmid-based measles vaccines offer great promises over the conventional fertilised egg method such as ease of manufacture and mimic wild-type intracellular antigen expression. The increasing number of clinical trials on plasmid-based measles vaccines has triggered the need to make more...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
BioMed Central Ltd.
2011
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/19178/1/Cultivation%20of%20E.pdf https://eprints.ums.edu.my/id/eprint/19178/7/Cultivation%20of%20E.%20coli%20carrying%20a%20plasmid-based%20Measles%20vaccine%20constructs%20%284.2%20kbp%20pcDNA3F%29%20employing%20medium%20optimisation%20and%20pH-temperature%20induction%20techniques.pdf |
| _version_ | 1796909817646809088 |
|---|---|
| author | Clarence M. Ongkudon Raelene Pickering Diane Webster Michael K. Danquah |
| author_facet | Clarence M. Ongkudon Raelene Pickering Diane Webster Michael K. Danquah |
| author_sort | Clarence M. Ongkudon |
| collection | UMS |
| description | Background
Plasmid-based measles vaccines offer great promises over the conventional fertilised egg method such as ease of manufacture and mimic wild-type intracellular antigen expression. The increasing number of clinical trials on plasmid-based measles vaccines has triggered the need to make more in less time.
Results
In this work, we investigated the process variables necessary to improve the volumetric and specific yields of a model plasmid-based measles vaccine (pcDNA3F) harboured in E. coli DH5α. Results from growth medium optimisation in 500 mL shake flasks by response surface methodology (RSM) generated a maximum volumetric yield of 13.65 mg/L which was 1.75 folds higher than that of the base medium. A controlled fed-batch fermentation employing strategic glycerol feeding and optimised growth conditions resulted in a remarkable pcDNA3F volumetric yield of 110 mg/L and a specific yield of 14 mg/g. In addition, growth pH modification and temperature fluctuation between 35 and 45°C were successfully employed to improve plasmid production.
Conclusion
Production of a high copy number plasmid DNA containing a foreign gene of interest is often hampered by the low plasmid volumetric yield which results from the over expression of foreign proteins and metabolic repressors. In this work, a simple bioprocess framework was employed and successfully improved the production of pcDNA3F |
| first_indexed | 2024-03-06T02:55:15Z |
| format | Article |
| id | ums.eprints-19178 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T02:55:15Z |
| publishDate | 2011 |
| publisher | BioMed Central Ltd. |
| record_format | dspace |
| spelling | ums.eprints-191782021-08-27T12:49:51Z https://eprints.ums.edu.my/id/eprint/19178/ Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques Clarence M. Ongkudon Raelene Pickering Diane Webster Michael K. Danquah QR Microbiology Background Plasmid-based measles vaccines offer great promises over the conventional fertilised egg method such as ease of manufacture and mimic wild-type intracellular antigen expression. The increasing number of clinical trials on plasmid-based measles vaccines has triggered the need to make more in less time. Results In this work, we investigated the process variables necessary to improve the volumetric and specific yields of a model plasmid-based measles vaccine (pcDNA3F) harboured in E. coli DH5α. Results from growth medium optimisation in 500 mL shake flasks by response surface methodology (RSM) generated a maximum volumetric yield of 13.65 mg/L which was 1.75 folds higher than that of the base medium. A controlled fed-batch fermentation employing strategic glycerol feeding and optimised growth conditions resulted in a remarkable pcDNA3F volumetric yield of 110 mg/L and a specific yield of 14 mg/g. In addition, growth pH modification and temperature fluctuation between 35 and 45°C were successfully employed to improve plasmid production. Conclusion Production of a high copy number plasmid DNA containing a foreign gene of interest is often hampered by the low plasmid volumetric yield which results from the over expression of foreign proteins and metabolic repressors. In this work, a simple bioprocess framework was employed and successfully improved the production of pcDNA3F BioMed Central Ltd. 2011 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19178/1/Cultivation%20of%20E.pdf text en https://eprints.ums.edu.my/id/eprint/19178/7/Cultivation%20of%20E.%20coli%20carrying%20a%20plasmid-based%20Measles%20vaccine%20constructs%20%284.2%20kbp%20pcDNA3F%29%20employing%20medium%20optimisation%20and%20pH-temperature%20induction%20techniques.pdf Clarence M. Ongkudon and Raelene Pickering and Diane Webster and Michael K. Danquah (2011) Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques. Microbial Cell Factories, 10 (16). pp. 1-10. ISSN 1475-2859 https://doi.org/10.1186/1475-2859-10-16 |
| spellingShingle | QR Microbiology Clarence M. Ongkudon Raelene Pickering Diane Webster Michael K. Danquah Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques |
| title | Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques |
| title_full | Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques |
| title_fullStr | Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques |
| title_full_unstemmed | Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques |
| title_short | Cultivation of E. coli carrying a plasmid-based Measles vaccine constructs (4.2 kbp pcDNA3F) employing medium optimisation and pH-temperature induction techniques |
| title_sort | cultivation of e coli carrying a plasmid based measles vaccine constructs 4 2 kbp pcdna3f employing medium optimisation and ph temperature induction techniques |
| topic | QR Microbiology |
| url | https://eprints.ums.edu.my/id/eprint/19178/1/Cultivation%20of%20E.pdf https://eprints.ums.edu.my/id/eprint/19178/7/Cultivation%20of%20E.%20coli%20carrying%20a%20plasmid-based%20Measles%20vaccine%20constructs%20%284.2%20kbp%20pcDNA3F%29%20employing%20medium%20optimisation%20and%20pH-temperature%20induction%20techniques.pdf |
| work_keys_str_mv | AT clarencemongkudon cultivationofecolicarryingaplasmidbasedmeaslesvaccineconstructs42kbppcdna3femployingmediumoptimisationandphtemperatureinductiontechniques AT raelenepickering cultivationofecolicarryingaplasmidbasedmeaslesvaccineconstructs42kbppcdna3femployingmediumoptimisationandphtemperatureinductiontechniques AT dianewebster cultivationofecolicarryingaplasmidbasedmeaslesvaccineconstructs42kbppcdna3femployingmediumoptimisationandphtemperatureinductiontechniques AT michaelkdanquah cultivationofecolicarryingaplasmidbasedmeaslesvaccineconstructs42kbppcdna3femployingmediumoptimisationandphtemperatureinductiontechniques |