Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia
Studies on eukaryotic signal transduction pathways have led to a variety of potential molecular targets for cancer therapy including Mitogen-activated Protein Kinase Kinase 1 (MKK1), Glycogen Synthase Kinase 3ß (GSK-3ß) and Type 1 Protein Phosphatase (PP1). The protein kinases and phosphatases in t...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Universiti Malaysia Sabah
2009
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| Online Access: | https://eprints.ums.edu.my/id/eprint/19240/1/Primary%20screening%20for%20natural%20inhibitors%20against%20eukaryotic%20signal%20transduction%20from%20local%20medicinal%20plants%20in%20Sabah.pdf |
| _version_ | 1796909825554120704 |
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| author | Jualang Azlan Gansau Goh, Keng Sean |
| author_facet | Jualang Azlan Gansau Goh, Keng Sean |
| author_sort | Jualang Azlan Gansau |
| collection | UMS |
| description | Studies on eukaryotic signal transduction pathways have led to a variety of potential molecular targets for cancer therapy including Mitogen-activated Protein Kinase
Kinase 1 (MKK1), Glycogen Synthase Kinase 3ß (GSK-3ß) and Type 1 Protein Phosphatase (PP1). The protein kinases and phosphatases in the signal transduction pathways play a vital role in mitogen activated protein (MAP) kinase signal transduction, tumorigenesis, apoptosis, and cancer metastasis. In this study, enetically engineered yeast strains were used as a model in the search for potential inhibitors against these signalling elements. Eleven species of medicinal plants were selected from various locations in Sabah, Malaysia, extracted and tested against MKK, GSK-3ß and PP1. Preliminary results showed the presence of potential MKK1 inhibitors in the crude extracts of Alphitonia excelsa, Cordyline terminalis and Tinospora crispa. However, no inhibitor was found against GSK-3ß and PP1. The crude extracts of Cordyline terminalis was further fractionated using a bioassay guided fractionation and four partially pure active fractions were isolated. |
| first_indexed | 2024-03-06T02:55:22Z |
| format | Article |
| id | ums.eprints-19240 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T02:55:22Z |
| publishDate | 2009 |
| publisher | Universiti Malaysia Sabah |
| record_format | dspace |
| spelling | ums.eprints-192402018-03-09T07:16:41Z https://eprints.ums.edu.my/id/eprint/19240/ Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia Jualang Azlan Gansau Goh, Keng Sean QR Microbiology Studies on eukaryotic signal transduction pathways have led to a variety of potential molecular targets for cancer therapy including Mitogen-activated Protein Kinase Kinase 1 (MKK1), Glycogen Synthase Kinase 3ß (GSK-3ß) and Type 1 Protein Phosphatase (PP1). The protein kinases and phosphatases in the signal transduction pathways play a vital role in mitogen activated protein (MAP) kinase signal transduction, tumorigenesis, apoptosis, and cancer metastasis. In this study, enetically engineered yeast strains were used as a model in the search for potential inhibitors against these signalling elements. Eleven species of medicinal plants were selected from various locations in Sabah, Malaysia, extracted and tested against MKK, GSK-3ß and PP1. Preliminary results showed the presence of potential MKK1 inhibitors in the crude extracts of Alphitonia excelsa, Cordyline terminalis and Tinospora crispa. However, no inhibitor was found against GSK-3ß and PP1. The crude extracts of Cordyline terminalis was further fractionated using a bioassay guided fractionation and four partially pure active fractions were isolated. Universiti Malaysia Sabah 2009 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19240/1/Primary%20screening%20for%20natural%20inhibitors%20against%20eukaryotic%20signal%20transduction%20from%20local%20medicinal%20plants%20in%20Sabah.pdf Jualang Azlan Gansau and Goh, Keng Sean (2009) Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia. Borneo Science, 21. pp. 37-46. ISSN 1394-4339 |
| spellingShingle | QR Microbiology Jualang Azlan Gansau Goh, Keng Sean Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia |
| title | Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia |
| title_full | Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia |
| title_fullStr | Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia |
| title_full_unstemmed | Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia |
| title_short | Primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in Sabah, Malaysia |
| title_sort | primary screening for natural inhibitors against eukaryotic signal transduction from local medicinal plants in sabah malaysia |
| topic | QR Microbiology |
| url | https://eprints.ums.edu.my/id/eprint/19240/1/Primary%20screening%20for%20natural%20inhibitors%20against%20eukaryotic%20signal%20transduction%20from%20local%20medicinal%20plants%20in%20Sabah.pdf |
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