Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah
Background: Escherichia coli sequence type 131 (ST131) has emerged among bacteria causing urinary tract infection (UTI) in the previous decade. This ST contains multiple drug resistant (MDR) genes together with genes encoding many virulence factors. As a result, this strain of uropathogenic E. col...
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| Format: | Article |
| Language: | English English |
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BRNSS Publication Hub
2016
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| Online Access: | https://eprints.ums.edu.my/id/eprint/19346/1/Detection%20of%20sequence%20type%20131%20in%20multi.pdf https://eprints.ums.edu.my/id/eprint/19346/7/Detection%20of%20Sequence%20Type%20131%20in.pdf |
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| author | Yun, Mei Lai Myo Thura Zaw Tin Sabai Aung Zaw, Lin |
| author_facet | Yun, Mei Lai Myo Thura Zaw Tin Sabai Aung Zaw, Lin |
| author_sort | Yun, Mei Lai |
| collection | UMS |
| description | Background: Escherichia coli sequence type 131 (ST131) has emerged among bacteria causing urinary tract
infection (UTI) in the previous decade. This ST contains multiple drug resistant (MDR) genes together with genes
encoding many virulence factors. As a result, this strain of uropathogenic E. coli (UPEC) gives rise to treatment
failure with consequent prolonged stay in a hospital. Therefore, earlier identification of this strain in the hospital
has advantage in combating severe type of UTI. Objective: To detect ST 131 strains in MDR UPEC isolates from
two hospitals of Sabah. Materials and Methods: Antibiotic susceptibility tests were performed to detect MDR
isolates. Two polymerase chain reactions (PCRs) including mdh and gyrB allelic-specific PCR were performed
on these MDR to detect ST131 strains. Results: The results showed four isolates were resistant to TMP-SMX,
gentamycin, ciprofloxacin, and cefotaxime, and three isolates of these were investigated to be ST131 clones by
two PCR reactions. Conclusion: There is the presence of ST131 strains in hospitals of Sabah. This information
will be a guideline for the clinician in the management of UTI in the clinical settings. |
| first_indexed | 2024-03-06T02:55:36Z |
| format | Article |
| id | ums.eprints-19346 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T02:55:36Z |
| publishDate | 2016 |
| publisher | BRNSS Publication Hub |
| record_format | dspace |
| spelling | ums.eprints-193462021-01-01T10:44:18Z https://eprints.ums.edu.my/id/eprint/19346/ Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah Yun, Mei Lai Myo Thura Zaw Tin Sabai Aung Zaw, Lin RS Pharmacy and materia medica Background: Escherichia coli sequence type 131 (ST131) has emerged among bacteria causing urinary tract infection (UTI) in the previous decade. This ST contains multiple drug resistant (MDR) genes together with genes encoding many virulence factors. As a result, this strain of uropathogenic E. coli (UPEC) gives rise to treatment failure with consequent prolonged stay in a hospital. Therefore, earlier identification of this strain in the hospital has advantage in combating severe type of UTI. Objective: To detect ST 131 strains in MDR UPEC isolates from two hospitals of Sabah. Materials and Methods: Antibiotic susceptibility tests were performed to detect MDR isolates. Two polymerase chain reactions (PCRs) including mdh and gyrB allelic-specific PCR were performed on these MDR to detect ST131 strains. Results: The results showed four isolates were resistant to TMP-SMX, gentamycin, ciprofloxacin, and cefotaxime, and three isolates of these were investigated to be ST131 clones by two PCR reactions. Conclusion: There is the presence of ST131 strains in hospitals of Sabah. This information will be a guideline for the clinician in the management of UTI in the clinical settings. BRNSS Publication Hub 2016-07 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19346/1/Detection%20of%20sequence%20type%20131%20in%20multi.pdf text en https://eprints.ums.edu.my/id/eprint/19346/7/Detection%20of%20Sequence%20Type%20131%20in.pdf Yun, Mei Lai and Myo Thura Zaw and Tin Sabai Aung and Zaw, Lin (2016) Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah. Asian Journal of Pharmaceutics, 10 (3). S421-S424. ISSN 1998-409X https://www.researchgate.net/publication/308625098_Detection_of_Sequence_Type_131_in_Multi-drug_Resistant_Uropathogenic_Escherichia_coli_Isolates_from_Two_Hospitals_of_Sabah |
| spellingShingle | RS Pharmacy and materia medica Yun, Mei Lai Myo Thura Zaw Tin Sabai Aung Zaw, Lin Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah |
| title | Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah |
| title_full | Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah |
| title_fullStr | Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah |
| title_full_unstemmed | Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah |
| title_short | Detection of sequence type 131 in multi-drug resistant uropathogenic Escherichia coli isolates from two hospitals of Sabah |
| title_sort | detection of sequence type 131 in multi drug resistant uropathogenic escherichia coli isolates from two hospitals of sabah |
| topic | RS Pharmacy and materia medica |
| url | https://eprints.ums.edu.my/id/eprint/19346/1/Detection%20of%20sequence%20type%20131%20in%20multi.pdf https://eprints.ums.edu.my/id/eprint/19346/7/Detection%20of%20Sequence%20Type%20131%20in.pdf |
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