Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)

Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an...

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Main Authors: Noor Aini Bohari, Shafiquzzaman Siddiquee, Kamaruzaman Hj. Ampon
Format: Article
Language:English
Published: MDPI AG, Basel, Switzerland 2016
Subjects:
Online Access:https://eprints.ums.edu.my/id/eprint/19364/1/Development%20of%20Formaldehyde%20Biosensor%20for%20Determination%20of%20Formalin%20in%20Fish%20Samples.pdf
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author Noor Aini Bohari
Shafiquzzaman Siddiquee
Kamaruzaman Hj. Ampon
author_facet Noor Aini Bohari
Shafiquzzaman Siddiquee
Kamaruzaman Hj. Ampon
author_sort Noor Aini Bohari
collection UMS
description Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), gold nanoparticles (AuNPs), and chitosan (CHIT), onto a glassy carbon electrode (GCE). The developed formaldehyde biosensor was analyzed for sensitivity, reproducibility, storage stability, and detection limits. Methylene blue was used as a redox indicator for increasing the electron transfer in the electrochemical cell. The developed biosensor measured the NADH electron from the NAD+ reduction at a potential of 0.4 V. Under optimal conditions, the differential pulse voltammetry (DPV) method detected a wider linear range of formaldehyde concentrations from 0.01 to 10 ppm within 5 s, with a detection limit of 0.1 ppm. The proposed method was successfully detected with the presence of formalin in fish samples, Lutjanus malabaricus and Thunnus Tonggol. The proposed method is a simple, rapid, and highly accurate, compared to the existing technique.
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spelling ums.eprints-193642018-03-13T03:24:50Z https://eprints.ums.edu.my/id/eprint/19364/ Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol) Noor Aini Bohari Shafiquzzaman Siddiquee Kamaruzaman Hj. Ampon TP Chemical technology Electrochemical biosensors are widely recognized in biosensing devices due to the fact that gives a direct, reliable, and reproducible measurement within a short period. During bio-interaction process and the generation of electrons, it produces electrochemical signals which can be measured using an electrochemical detector. A formaldehyde biosensor was successfully developed by depositing an ionic liquid (IL) (e.g., 1-ethyl-3-methylimidazolium trifluoromethanesulfonate ([EMIM][Otf])), gold nanoparticles (AuNPs), and chitosan (CHIT), onto a glassy carbon electrode (GCE). The developed formaldehyde biosensor was analyzed for sensitivity, reproducibility, storage stability, and detection limits. Methylene blue was used as a redox indicator for increasing the electron transfer in the electrochemical cell. The developed biosensor measured the NADH electron from the NAD+ reduction at a potential of 0.4 V. Under optimal conditions, the differential pulse voltammetry (DPV) method detected a wider linear range of formaldehyde concentrations from 0.01 to 10 ppm within 5 s, with a detection limit of 0.1 ppm. The proposed method was successfully detected with the presence of formalin in fish samples, Lutjanus malabaricus and Thunnus Tonggol. The proposed method is a simple, rapid, and highly accurate, compared to the existing technique. MDPI AG, Basel, Switzerland 2016 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19364/1/Development%20of%20Formaldehyde%20Biosensor%20for%20Determination%20of%20Formalin%20in%20Fish%20Samples.pdf Noor Aini Bohari and Shafiquzzaman Siddiquee and Kamaruzaman Hj. Ampon (2016) Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol). Biosensors, 6 (3). p. 32. ISSN 2079-6374 https://doi.org/10.3390/bios6030032
spellingShingle TP Chemical technology
Noor Aini Bohari
Shafiquzzaman Siddiquee
Kamaruzaman Hj. Ampon
Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)
title Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)
title_full Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)
title_fullStr Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)
title_full_unstemmed Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)
title_short Development of Formaldehyde Biosensor for Determination of Formalin in Fish Samples; Malabar Red Snapper (Lutjanus malabaricus) and Longtail Tuna (Thunnus tonggol)
title_sort development of formaldehyde biosensor for determination of formalin in fish samples malabar red snapper lutjanus malabaricus and longtail tuna thunnus tonggol
topic TP Chemical technology
url https://eprints.ums.edu.my/id/eprint/19364/1/Development%20of%20Formaldehyde%20Biosensor%20for%20Determination%20of%20Formalin%20in%20Fish%20Samples.pdf
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AT shafiquzzamansiddiquee developmentofformaldehydebiosensorfordeterminationofformalininfishsamplesmalabarredsnapperlutjanusmalabaricusandlongtailtunathunnustonggol
AT kamaruzamanhjampon developmentofformaldehydebiosensorfordeterminationofformalininfishsamplesmalabarredsnapperlutjanusmalabaricusandlongtailtunathunnustonggol