The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats
Diabetes is a major public health problem. The new therapies developments that are able to Improve glycemia management, cure diabetes and even protect from it are of great interest. This study investigated the effect of selected plant extracts on the Insulin gene expression profile against STZ- i...
| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
2014
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| Online Access: | https://eprints.ums.edu.my/id/eprint/19571/1/The%20effect%20of%20selected%20plant%20extracts.pdf |
| _version_ | 1825713410467168256 |
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| author | Valentino Milton Junior Gumbilai |
| author_facet | Valentino Milton Junior Gumbilai |
| author_sort | Valentino Milton Junior Gumbilai |
| collection | UMS |
| description | Diabetes is a major public health problem. The new therapies developments that are
able to Improve glycemia management, cure diabetes and even protect from it are of
great interest. This study investigated the effect of selected plant extracts on the
Insulin gene expression profile against STZ- induced pancreatic cell damages.
Eighteen rats were divided into six groups: (i) Nonnal control rats; (ii) diabetic
control rats, diabetic rats administered with (iii) Momorriica Charantia, (iv) Morinda
Citrifolia, (v) Parkia Speciosa and (vi)Metformin. All rats were given treatment daily
for 3 days before the STZ injection and for 1 subsequent week thereafter. M.
charantia extract showed the highest significant value of total phenolic content
(292.13 mg/g) compared to M. citrifolia and P. speciosa extracts. The DPPH
scavenging rate for M. charantia extract was ranging from 4.3% to 51.8% followed
by M. dtrifolia extracts (1.8% to 57.9%) and P. speciosa extract (0.4% to 48.7%).
The result showed that M. charantia extract has the highest antioxidant activity with
EC₅₀ value was 356.56 µg/ mi. M. charantia extract also showed the highest value in
the total flavanoid content (14.56 mg/g) compared to other two samples. Expression
of both GAPDH as the reference gene and Ins2 gene as the target gene was
measured by SYBR Green I fluorescence; melting curves and agarose gel
electrophoresis were used to verify the specificity of gene (Ins2) for diabetic rats
treated with M. charantia extracts expressing fold differences of 32 fold expression
compare to the diabetic control rats only. This study report the preliminary study of
the effect of plant extracts on the pancreatic cell insulin gene expression profile. The
results represent an important step toward improved understanding of the regulation
of insulin secretion on insulin 2 (1ns2) genes. |
| first_indexed | 2024-03-06T02:56:08Z |
| format | Thesis |
| id | ums.eprints-19571 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T02:56:08Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ums.eprints-195712018-03-27T06:32:44Z https://eprints.ums.edu.my/id/eprint/19571/ The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats Valentino Milton Junior Gumbilai RC Internal medicine Diabetes is a major public health problem. The new therapies developments that are able to Improve glycemia management, cure diabetes and even protect from it are of great interest. This study investigated the effect of selected plant extracts on the Insulin gene expression profile against STZ- induced pancreatic cell damages. Eighteen rats were divided into six groups: (i) Nonnal control rats; (ii) diabetic control rats, diabetic rats administered with (iii) Momorriica Charantia, (iv) Morinda Citrifolia, (v) Parkia Speciosa and (vi)Metformin. All rats were given treatment daily for 3 days before the STZ injection and for 1 subsequent week thereafter. M. charantia extract showed the highest significant value of total phenolic content (292.13 mg/g) compared to M. citrifolia and P. speciosa extracts. The DPPH scavenging rate for M. charantia extract was ranging from 4.3% to 51.8% followed by M. dtrifolia extracts (1.8% to 57.9%) and P. speciosa extract (0.4% to 48.7%). The result showed that M. charantia extract has the highest antioxidant activity with EC₅₀ value was 356.56 µg/ mi. M. charantia extract also showed the highest value in the total flavanoid content (14.56 mg/g) compared to other two samples. Expression of both GAPDH as the reference gene and Ins2 gene as the target gene was measured by SYBR Green I fluorescence; melting curves and agarose gel electrophoresis were used to verify the specificity of gene (Ins2) for diabetic rats treated with M. charantia extracts expressing fold differences of 32 fold expression compare to the diabetic control rats only. This study report the preliminary study of the effect of plant extracts on the pancreatic cell insulin gene expression profile. The results represent an important step toward improved understanding of the regulation of insulin secretion on insulin 2 (1ns2) genes. 2014 Thesis NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/19571/1/The%20effect%20of%20selected%20plant%20extracts.pdf Valentino Milton Junior Gumbilai (2014) The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats. Masters thesis, Universiti Malaysia Sabah. |
| spellingShingle | RC Internal medicine Valentino Milton Junior Gumbilai The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats |
| title | The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats |
| title_full | The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats |
| title_fullStr | The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats |
| title_full_unstemmed | The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats |
| title_short | The effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin-induced diabetic rats |
| title_sort | effect of selected plant extracts on the expression prifiles of the insulin 2 gene in pancreatic cells of streptozotocin induced diabetic rats |
| topic | RC Internal medicine |
| url | https://eprints.ums.edu.my/id/eprint/19571/1/The%20effect%20of%20selected%20plant%20extracts.pdf |
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