Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah
Helicobacter pylori is a pathogenic bacterium that has been associated with peptic ulcer disease and cancers of human gastrointestinal tract. Currently, c1arithromycin has remained as one of the most powerful antibiotics used as first line treatment for H. pylori infection. In Malaysia, 2.14% of...
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| Format: | Academic Exercise |
| Language: | English |
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2014
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| Online Access: | https://eprints.ums.edu.my/id/eprint/19585/1/Molecular%20detection%20of%20helicobacter%20pylori%20.pdf |
| _version_ | 1825713412985847808 |
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| author | Nurul Ashira Azman |
| author_facet | Nurul Ashira Azman |
| author_sort | Nurul Ashira Azman |
| collection | UMS |
| description | Helicobacter pylori is a pathogenic bacterium that has been associated with peptic ulcer
disease and cancers of human gastrointestinal tract. Currently, c1arithromycin has
remained as one of the most powerful antibiotics used as first line treatment for H. pylori
infection. In Malaysia, 2.14% of c1arithromycin resistance H. pylori cases were reported.
The small percentage indicates the low incidence of c1arithromycin resistance H. pylori in
this country. This study aims to detect A2142G and A2143G mutations of 235 rRNA gene
in H. pylori isolated from 5abah and to determine prevalence of c1arithromycin resistance
in 5abah isolates using polymerase chain reaction-based restriction fragment length
polymorphism (PCR-RFLP). campylobacter-like organism (CLO) tested gastric biopsies
were collected from Queen Elizabeth Hospital and subjected for DNA extraction. PCR
amplification of 165 rRNA was performed to confirm the presence of H. pylori in the
extracted DNA. Next, 235 rRNA gene was amplified and preceded for restriction
fragment length polymorphism (RFLP) by restriction enzyme BsaI and MboII to study
A2142G and A2143G mutations. From 67 gastric biopsies evaluated, only 41 were
detected to be H. pylori positivity. Among 41 H. pylori positivity extracts, 29 isolates
were successfully amplified at region 235 rRNA gene. Total two isolates has been
detected with A2142G mutation and another one with A2143G mutation out of 25
isolates. Low-level of resistance was detected in Sabah isolates with only 4% of cases.
This showed the uncommon occurrence in the local strain. This might due to the less
usage of this antibiotic for H. pylori eradication treatment in this region. Therefore,
clarithromycin can be continued as the first line drug to treat H. pylori strain. PCR-RFLP
is one the reliable approaches in detecting mutations in H. pylori clarithromycin resistant
strain |
| first_indexed | 2024-03-06T02:56:11Z |
| format | Academic Exercise |
| id | ums.eprints-19585 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T02:56:11Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ums.eprints-195852018-03-27T07:25:03Z https://eprints.ums.edu.my/id/eprint/19585/ Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah Nurul Ashira Azman TP Chemical technology Helicobacter pylori is a pathogenic bacterium that has been associated with peptic ulcer disease and cancers of human gastrointestinal tract. Currently, c1arithromycin has remained as one of the most powerful antibiotics used as first line treatment for H. pylori infection. In Malaysia, 2.14% of c1arithromycin resistance H. pylori cases were reported. The small percentage indicates the low incidence of c1arithromycin resistance H. pylori in this country. This study aims to detect A2142G and A2143G mutations of 235 rRNA gene in H. pylori isolated from 5abah and to determine prevalence of c1arithromycin resistance in 5abah isolates using polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP). campylobacter-like organism (CLO) tested gastric biopsies were collected from Queen Elizabeth Hospital and subjected for DNA extraction. PCR amplification of 165 rRNA was performed to confirm the presence of H. pylori in the extracted DNA. Next, 235 rRNA gene was amplified and preceded for restriction fragment length polymorphism (RFLP) by restriction enzyme BsaI and MboII to study A2142G and A2143G mutations. From 67 gastric biopsies evaluated, only 41 were detected to be H. pylori positivity. Among 41 H. pylori positivity extracts, 29 isolates were successfully amplified at region 235 rRNA gene. Total two isolates has been detected with A2142G mutation and another one with A2143G mutation out of 25 isolates. Low-level of resistance was detected in Sabah isolates with only 4% of cases. This showed the uncommon occurrence in the local strain. This might due to the less usage of this antibiotic for H. pylori eradication treatment in this region. Therefore, clarithromycin can be continued as the first line drug to treat H. pylori strain. PCR-RFLP is one the reliable approaches in detecting mutations in H. pylori clarithromycin resistant strain 2014 Academic Exercise NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/19585/1/Molecular%20detection%20of%20helicobacter%20pylori%20.pdf Nurul Ashira Azman (2014) Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah. Universiti Malaysia Sabah. (Unpublished) |
| spellingShingle | TP Chemical technology Nurul Ashira Azman Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah |
| title | Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah |
| title_full | Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah |
| title_fullStr | Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah |
| title_full_unstemmed | Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah |
| title_short | Molecular detection of Helicobacter pylori resistance to clarithromycin isolated from Sabah |
| title_sort | molecular detection of helicobacter pylori resistance to clarithromycin isolated from sabah |
| topic | TP Chemical technology |
| url | https://eprints.ums.edu.my/id/eprint/19585/1/Molecular%20detection%20of%20helicobacter%20pylori%20.pdf |
| work_keys_str_mv | AT nurulashiraazman moleculardetectionofhelicobacterpyloriresistancetoclarithromycinisolatedfromsabah |