Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization
A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enz...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English English |
| Published: |
Springer Berlin Heidelberg
2015
|
| Subjects: | |
| Online Access: | https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf |
| _version_ | 1825713415108165632 |
|---|---|
| author | Mailin Misson Bo, Jin Binghui, Chen Hu, Zhang |
| author_facet | Mailin Misson Bo, Jin Binghui, Chen Hu, Zhang |
| author_sort | Mailin Misson |
| collection | UMS |
| description | A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications. |
| first_indexed | 2024-03-06T02:56:13Z |
| format | Article |
| id | ums.eprints-19596 |
| institution | Universiti Malaysia Sabah |
| language | English English |
| last_indexed | 2024-03-06T02:56:13Z |
| publishDate | 2015 |
| publisher | Springer Berlin Heidelberg |
| record_format | dspace |
| spelling | ums.eprints-195962020-12-09T02:50:13Z https://eprints.ums.edu.my/id/eprint/19596/ Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization Mailin Misson Bo, Jin Binghui, Chen Hu, Zhang TA Engineering (General). Civil engineering (General) A functionalized polystyrene nanofiber (PSNF) immobilized β-galactosidase assembly (PSNF-Gal) was synthesized as a nanobiocatalyst aiming to enhance the biocatalyst stability and functional ability. The PSNF fabricated by electrospinning was functionalized through a chemical oxidation method for enzyme binding. The bioengineering performance of the enzyme carriers was further evaluated for bioconversion of lactose to galacto-oligosaccharides (GOS). The modified PSNF-Gal demonstrated distinguished performances to preserve the same activity as the free β-galactosidase at the optimum pH of 7.0, and to enhance the enzyme stability of PSNF-Gal in an alkaline condition up to pH 10. The PSNF assembly demonstrated improved thermal stability from 37 to 60 °C. The nanobiocatalyst was able to retain 30 % of its initial activity after ninth operation cycles comparing to four cycles with the unmodified counterpart. In contrast with free β-galactosidase, the modified PSNF-Gal enhanced the GOS yield from 14 to 28 %. These findings show the chemically modified PSNF-based nanobiocatalyst may be pertinent for various enzyme-catalysed bioprocessing applications. Springer Berlin Heidelberg 2015-10 Article PeerReviewed text en https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf text en https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf Mailin Misson and Bo, Jin and Binghui, Chen and Hu, Zhang (2015) Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization. Bioprocess and Biosystems Engineering, 38 (10). pp. 1915-1923. ISSN 1615-7605 https://doi.org/10.1007/s00449-015-1432-5 |
| spellingShingle | TA Engineering (General). Civil engineering (General) Mailin Misson Bo, Jin Binghui, Chen Hu, Zhang Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
| title | Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
| title_full | Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
| title_fullStr | Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
| title_full_unstemmed | Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
| title_short | Enhancing enzyme stability and metabolic functional ability of β-galactosidase through functionalized polymer nanofiber immobilization |
| title_sort | enhancing enzyme stability and metabolic functional ability of β galactosidase through functionalized polymer nanofiber immobilization |
| topic | TA Engineering (General). Civil engineering (General) |
| url | https://eprints.ums.edu.my/id/eprint/19596/1/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2.pdf https://eprints.ums.edu.my/id/eprint/19596/7/Enhancing%20enzyme%20stability%20and%20metabolic%20functional%20ability%20of%20%CE%B2-galactosidase%20through%20functionalized%20polymer%20nanofiber%20immobilization.pdf |
| work_keys_str_mv | AT mailinmisson enhancingenzymestabilityandmetabolicfunctionalabilityofbgalactosidasethroughfunctionalizedpolymernanofiberimmobilization AT bojin enhancingenzymestabilityandmetabolicfunctionalabilityofbgalactosidasethroughfunctionalizedpolymernanofiberimmobilization AT binghuichen enhancingenzymestabilityandmetabolicfunctionalabilityofbgalactosidasethroughfunctionalizedpolymernanofiberimmobilization AT huzhang enhancingenzymestabilityandmetabolicfunctionalabilityofbgalactosidasethroughfunctionalizedpolymernanofiberimmobilization |