Comparison of DNA extraction methods from orchid leaves
DNA extraction is an important step in obtaining molecular data. In this study, three DNA extraction methods were compared based on the quantity and quality of the obtained DNA. The Dellaporta, SDS and CTAB methods were used to extract DNA from leaf samples of four different orchid species namely G...
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| Format: | Academic Exercise |
| Language: | English |
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2008
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| Online Access: | https://eprints.ums.edu.my/id/eprint/19812/1/Comparison%20of%20DNA%20extraction%20methods%20from%20orchid%20leaves.pdf |
| _version_ | 1825713440937738240 |
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| author | Gayathri Priyatharlsini Vythalingam |
| author_facet | Gayathri Priyatharlsini Vythalingam |
| author_sort | Gayathri Priyatharlsini Vythalingam |
| collection | UMS |
| description | DNA extraction is an important step in obtaining molecular data. In this study, three DNA extraction methods were compared based on the quantity and quality of the obtained
DNA. The Dellaporta, SDS and CTAB methods were used to extract DNA from leaf samples of four different orchid species namely G. speciosum, P. amahilis, R. bella and
Oncidillm species with each having four replicates. Results indicated that there was interaction between the extraction methods and the species used in terms of the obtained
DNA quantity (p<0.05).However, there was no significant interaction between the methods and species in terms of quality (p>O.05). Based on the results the highest mean
of DNA quantity for the CTAB method was obta.i 'n ed for G. speciosum (84.40 ug).Furthermore, for the Dellaporta method the highest mean quantity was obtained by Oncidium species (48.10 ug). Apart from that, the highest mean quantity for the SDS method was obtained by G. speciosum (30.95 ug). On the other hand, based on the quality of the obtained DNA, the Dellaporta and CT AB method gave the highest mean ratio of A260 / A280 for aU the species with mean values of 1.678 and 1.582 respectively. Conversely, the SDS method gave the lowest mean ratio of 1.323. |
| first_indexed | 2024-03-06T02:56:38Z |
| format | Academic Exercise |
| id | ums.eprints-19812 |
| institution | Universiti Malaysia Sabah |
| language | English |
| last_indexed | 2024-03-06T02:56:38Z |
| publishDate | 2008 |
| record_format | dspace |
| spelling | ums.eprints-198122018-04-19T03:36:45Z https://eprints.ums.edu.my/id/eprint/19812/ Comparison of DNA extraction methods from orchid leaves Gayathri Priyatharlsini Vythalingam QP Physiology DNA extraction is an important step in obtaining molecular data. In this study, three DNA extraction methods were compared based on the quantity and quality of the obtained DNA. The Dellaporta, SDS and CTAB methods were used to extract DNA from leaf samples of four different orchid species namely G. speciosum, P. amahilis, R. bella and Oncidillm species with each having four replicates. Results indicated that there was interaction between the extraction methods and the species used in terms of the obtained DNA quantity (p<0.05).However, there was no significant interaction between the methods and species in terms of quality (p>O.05). Based on the results the highest mean of DNA quantity for the CTAB method was obta.i 'n ed for G. speciosum (84.40 ug).Furthermore, for the Dellaporta method the highest mean quantity was obtained by Oncidium species (48.10 ug). Apart from that, the highest mean quantity for the SDS method was obtained by G. speciosum (30.95 ug). On the other hand, based on the quality of the obtained DNA, the Dellaporta and CT AB method gave the highest mean ratio of A260 / A280 for aU the species with mean values of 1.678 and 1.582 respectively. Conversely, the SDS method gave the lowest mean ratio of 1.323. 2008 Academic Exercise NonPeerReviewed text en https://eprints.ums.edu.my/id/eprint/19812/1/Comparison%20of%20DNA%20extraction%20methods%20from%20orchid%20leaves.pdf Gayathri Priyatharlsini Vythalingam (2008) Comparison of DNA extraction methods from orchid leaves. Universiti Malaysia Sabah. (Unpublished) |
| spellingShingle | QP Physiology Gayathri Priyatharlsini Vythalingam Comparison of DNA extraction methods from orchid leaves |
| title | Comparison of DNA extraction methods from orchid leaves |
| title_full | Comparison of DNA extraction methods from orchid leaves |
| title_fullStr | Comparison of DNA extraction methods from orchid leaves |
| title_full_unstemmed | Comparison of DNA extraction methods from orchid leaves |
| title_short | Comparison of DNA extraction methods from orchid leaves |
| title_sort | comparison of dna extraction methods from orchid leaves |
| topic | QP Physiology |
| url | https://eprints.ums.edu.my/id/eprint/19812/1/Comparison%20of%20DNA%20extraction%20methods%20from%20orchid%20leaves.pdf |
| work_keys_str_mv | AT gayathripriyatharlsinivythalingam comparisonofdnaextractionmethodsfromorchidleaves |